Characterization of the humoral immune response in alpacas (Lama pacos) experimentally infected with Fasciola hepatica against cysteine proteinases Fas1 and Fas2 and histopathological findings

Timoteo, Olga S.; Maco, Vicente; Neyra, Víctor; Yi, Pedro; Leguía, G.; Espinoza, J.R.

doi:10.1016/j.vetimm.2005.01.007

Cited by 20 publications

(12 citation statements)

References 16 publications

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“…Other antigens are gut-associated highly glycosylated proteins 1 and yet others are cysteine proteinases 3,5,6,9,26,27 successfully used in immunodiagnosis 4,11,21,29 . However, specific bands within the range of 23-30 kDa were not detected in our FhTSE, perhaps due to non use of purification procedures.…”

Section: Discussionmentioning

confidence: 99%

Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera

Almeida

Ferreira

Planchart

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYFascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE) and the adult worm vomit (FhAWV) in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F), other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis.

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Section: Discussionmentioning

confidence: 99%

Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera

Almeida

Ferreira

Planchart

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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“…Una vez en los conductos biliares, el parásito adulto causa hiperplasia de las paredes con marcada fibrosis (20,21) y extensivo daño en la arquitectura hepática causada por la secreción de enzimas parasitarias que destruyen el parénquima hepático (22) . Los síntomas reflejan la obstrucción biliar causada por los parásitos adultos, si esta es parcial o si el parásito ingresa a la vesícula biliar, los síntomas son dolor crónico en el hipocondrio derecho o en abdomen superior semejante al cuadro de colecistitis crónica reagudizada causada por cálculos biliares (23,24) ; y si la obstrucción es completa, se presenta ictericia y requiere de cirugía o colangiopancreatografía retrógrada endoscópica de emergencia.…”

Section: Infección Humana Crónicaunclassified

“…Es la técnica de elección para el diagnóstico de la fasciolosis humana. Dado que el parásito tiene una alta inmunogenicidad, se producen tempranamente anticuerpos específicos contra los antígenos parasitarios, permitiendo la detección en la fase aguda dos semanas después de la infección (22) . Uno de los métodos de detección serológica es FAS2-ELISA ® , una prueba serológica validada que detecta anticuerpos inmunoglobulina G del suero contra el antígeno FAS2 del parásito adulto (29,30) y es útil para el diagnóstico de la fasciolosis humana aguda, crónica (29) y ectópica (31) .…”

Section: Diagnóstico En Humanos Por Serologíaunclassified

Fasciolosis humana y animal en el Perú: impacto en la economía de las zonas endémicas

Espinoza¹,

Terashima²,

Herrera-Velit³

et al. 2010

Rev Peru Med Exp Salud Publica

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Fasciola hepatica is the causative agent of fasciolosis in Peru; the disease is an important public health problem by the high prevalence of the human infection affecting mainly children and a major veterinary problem by the high rates of infected livestock. The human disease is endemic in the Sierra and the Coast but sporadic in the Amazonia, and reported in 18 Departments, while the animal infection in 21 of 24 Departments of Peru. Transmission occurs in Andean rural populations engaged in agriculture, but recently an increasing number of people became infected in the cities. The epidemiological situation in Peru includes i) Departments with non-autochtonous cases, where infection occurs by consumption of contaminated vegetables brought from endemic areas or infection is acquired by visit to endemic areas; ii) Departments with hypoendemic and mesoendemic villages, where transmission occurs by ingest of contaminated vegetables and prevalence ≤10%; and iii) Departments with hyper-endemic villages with human prevalence >10 %, with an intense transmission by consumption of contaminated vegetables. The disease affects bovine, sheep, goat, swine, equine, South American camelids, rabbits and guinea pigs. The negative impact of fasciolosis in the livestock economy is not lesser than US$ 50 million per year, estimation based on the prevalence and the number of condemned livers in the abattoirs. It is difficult to estimate the economic impact of this infection in the human health due to its status of neglected disease, but fasciolosis is hyper-endemic in the poorest Andean areas of Peru where the situation has to be recognized as a public health emergency.

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“…The disease includes 4 clinical periods: (1) Incubation phase, from the ingestion of metacercariae to the first symptoms; (2) Acute phase, migration of the fluke to the bile ducs, with symptoms of fever, abdominal pain, respiratoy symptoms, gastrointestinal disturbances, hepatomegaly, splenomegaly, ascites, anemia, and jaundice; (3) Latent phase, maturation of the parasites and starting of ovoposition with eosinophilia, gastrointestinal complaints and relapses of acute symptoms; (4) Chronic phase with bile duct obstruction, cholecystitis, cholangitis, liver abscess, hemorrhage, lithiasis, and cirrhosis. The chronic stage may be asymptomatic and extended for years if the disease remains untreated [14,18] . Our patients were compatible with the acute phase in terms of symptoms, but some were in the latent phase due to eosinophilia and the laboratory findings.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cases with Fasciola hepatica infection: experience in 6 children

Tezer

Yüksek

Parlakay

et al. 2013

Asian Pacific Journal of Tropical Disease

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Characterization of the humoral immune response in alpacas (Lama pacos) experimentally infected with Fasciola hepatica against cysteine proteinases Fas1 and Fas2 and histopathological findings

Cited by 20 publications

References 16 publications

Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera

Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera

Fasciolosis humana y animal en el Perú: impacto en la economía de las zonas endémicas

Evaluation of cases with Fasciola hepatica infection: experience in 6 children

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