Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera

Abstract

SUMMARYFascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE) and the adult worm vomit (FhAWV) in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F), other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis.

Detection of Fasciola hepatica and Fasciola gigantica common and uncommon antigens, using rabbit hyper immune serum raised against their excretory–secretory and somatic antigens

“…Differences in number and molecular weight of reported protein bands of ES or In study of Gonence and Sarimehmetoglu (2004) serum of infected sheep, with F. hepatica, reacted with protein bands of 24,33,35,[44][45][46][47][48][49][50][51][52][53][54][55], and 66 kDa of F. hepatica somatic antigen, and protein bands of 24, 33, 39.5, 42 and 44-55 kDa of F. hepatica ES antigen. In other study, serum of human infected with F. hepatica reacted with bands of 8, 12, 15, and 24 kDa in western blotting on F. hepatica vomiting antigen (De Almeida et al 2007). In our study, rabbit polyclonal antibodies to F. hepatica ES antigen detected 11 bands in F. hepatica somatic and ES antigen, most of them are different from those reported by De Almeida et al (2007).…”

“…In other study, serum of human infected with F. hepatica reacted with bands of 8, 12, 15, and 24 kDa in western blotting on F. hepatica vomiting antigen (De Almeida et al 2007). In our study, rabbit polyclonal antibodies to F. hepatica ES antigen detected 11 bands in F. hepatica somatic and ES antigen, most of them are different from those reported by De Almeida et al (2007).…”

“…Eight protein bands reported in their study are common with those that we are seeing in the current study. De Almeida et al (2007) reported 17 protein bands (ranging from 2 to 80 kDa) in F. hepatica somatic antigen (De Almeida et al 2007). Meshgi et al (2008) reported six common protein bands (ranging from 15 to 42 kDa) for SDS PAGE pattern of both F. hepatica and F. gigantica ES antigens.…”

“…In a study by Gonenc et al (2004) 23 protein bands (ranging from 6.5 to 205 kDa) for F. hepatica ES antigen have been reported. De Almeida et al reported 19 protein bands (ranging from 2 to 80 kDa) for F. hepatica vomiting antigen (ES antigen) (De Almeida et al 2007). In the current study, five and nine protein bands have been respectively detected in F. hepatica and F. gigantica ES antigens.…”

Purificación de la fracción antigénica 27-28 KDA a partir del antígeno metabólico secretado excretado de Fasciola hepatica

“…Algunos análisis electroforéticos y bioquímicos del antígeno total AgMFhE/S de F. hepatica, mostraron 31 bandas de naturaleza polipeptídica en rango de 10-116 KDa, dentro de los cuales destacan un grupo de siete polipéptidos entre 66-116 KDa; un segundo grupo de nueve polipéptidos entre 60-31 KDa, un tercero con seis polipéptidos entre 29-17 KDa, y una agrupación de nueve polipéptidos entre 10-15 KDa (3,4) . Estos antígenos son utilizados en pruebas serológicas con enfoque diagnóstico, con valores variables de sensibilidad y especificidad (5,6) . Se conocen cuatro bandas de diagnóstico específicas (8, 12, 15 y 24kDa) en pacientes infectados con F. hepatica (6) .…”