Characterization of the indigenous PAH-degrading bacteria of Spartina dominated salt marshes in the New York/New Jersey Harbor

Launen, Loren A.; Dutta, Jayeeta; Turpeinen, Riina; Eastep, Michael E.; Dorn, Ruth; Buggs, Vincent H.; Leonard, J.W.; Häggblom, Max M.

doi:10.1007/s10532-007-9141-7

Cited by 20 publications

(10 citation statements)

References 37 publications

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“…Similarly, we found here that the general decrease in bacterial diversity under phenanthrene was concomitant to an increase in the relative abundance of putative PAH degraders, and more specifically for the genera Mycobacterium , Cycloclasticus and Massilia . These genera represent large bacterial groups that are able to metabolize and degrade PAH (33, 34) and are consistent with previous results about the PAH-degrading bacteria associated to Spartina in salt marshes (35, 36). Interestingly, Cycloclasticus spp.…”

Section: Discussionsupporting

confidence: 91%

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Cavé‐Radet¹,

Monard²,

Amrani³

et al. 2019

Preprint

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14Spartina spp. are widely distributed salt marsh plants that have a recent history of 15 hybridization and polyploidization. These evolutionary events have resulted in species with a 16 heightened resilience to hydrocarbon contamination, which could make them an ideal model 17 plant for the phytoremediation/reclamation of contaminated coastal ecosystems. However, it 18 is still unknown if allopolyploidization events also resulted in differences in the plant 19 rhizosphere-associated microbial communities, and if this could improve the plant 20 phytoremediation potential. Here, we grew two parental Spartina species, their hybrid and the 21 resulting allopolyploid in salt marsh sediments that were contaminated or not with 22 phenanthrene, a model tricyclic PAH. The DNA from the rhizosphere soil was extracted and 23 the bacterial 16S rRNA gene and ITS region were amplified and sequenced. Generally, both 24 the presence of phenanthrene and the identity of the plant species had significant influences 25 on the bacterial and fungal community structure, composition and diversity. In particular, the 26 allopolyploid S. anglica, harbored a more diverse bacterial community in its rhizosphere, and 27 relatively higher abundance of various bacterial and fungal taxa. Putative hydrocarbon 28 degraders were significantly more abundant in the rhizosphere soil contaminated with 29 phenanthrene, with the Nocardia genus being significantly more abundant in the rhizosphere 30 of S. anglica. Overall our results are showing that the recent polyploidization events in the 31 Spartina did influence the rhizosphere microbiome, both under normal and contaminated 32 conditions, but more work will be necessary to confirm if these differences result in a higher 33 phytoremediation potential. 34 35 36 3 Importance 37Salt marshes are at the forefront of coastal contamination events caused by marine oil spills. 38Microbes in these environments play a key role in the natural attenuation of these 39 contamination events, often in association with plant roots. One such plant is the Spartina, 40 which are widely distributed salt marsh plants. Intriguingly, some species of the Spartina 41 show heightened resistance to contamination, which we hypothesized to be due to differences 42 in their microbiota. This was indeed the case, with the most resistant Spartina also showing 43 the most different microbiota. A better understanding of the relationships between the 44 Spartina and their microbiota could improve the coastal oil spill clean-up strategies and 45 provide green alternatives to more traditional physico-chemical approaches.46 47 48 49 Polycyclic aromatic hydrocarbons (PAHs) are a group of ubiquitous organic pollutants, that 50 are highly concerning in view of their potentially severe impact on natural ecosystems and 51 public health. The remediation of sites contaminated by these compounds due to human 52 activity, such as oil spill, is mainly carried out through excavation, soil leaching or various 53 techniques based on microbial degra...

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Section: Discussionsupporting

confidence: 91%

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Cavé‐Radet¹,

Monard²,

Amrani³

et al. 2019

Preprint

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“…Additionally, comprehensive environmental screening surveys that have resulted in the isolation and identification of numerous HMW PAH-degrading bacteria representing different genera from diverse locations throughout the world have been conducted (Mueller et al, 1997;Aitken et al, 1998;Ho et al, 2000;Zhou et al, 2006;Seo et al, 2007;Hilyard et al, 2008;Launen et al, 2008;Zhou et al, 2008).…”

Section: Reports Of Hmw Pah Biodegradation By Bacteria Other Than Mycmentioning

confidence: 99%

Advances in the field of high‐molecular‐weight polycyclic aromatic hydrocarbon biodegradation by bacteria

Kanaly

Harayama

2010

Microbial Biotechnology

237

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SummaryInterest in understanding prokaryotic biotransformation of high‐molecular‐weight polycyclic aromatic hydrocarbons (HMW PAHs) has continued to grow and the scientific literature shows that studies in this field are originating from research groups from many different locations throughout the world. In the last 10 years, research in regard to HMW PAH biodegradation by bacteria has been further advanced through the documentation of new isolates that represent diverse bacterial types that have been isolated from different environments and that possess different metabolic capabilities. This has occurred in addition to the continuation of in‐depth comprehensive characterizations of previously isolated organisms, such as Mycobacterium vanbaalenii PYR‐1. New metabolites derived from prokaryotic biodegradation of four‐ and five‐ring PAHs have been characterized, our knowledge of the enzymes involved in these transformations has been advanced and HMW PAH biodegradation pathways have been further developed, expanded upon and refined. At the same time, investigation of prokaryotic consortia has furthered our understanding of the capabilities of microorganisms functioning as communities during HMW PAH biodegradation.

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“…Although the highest PAH concentrations, in total or as individual PAH compounds, were below the concentrations thought to induce toxic effects (76), this PAH source transition likely impacted the microbial communities because petrogenic PAHs are generally more bioavailable than pyrogenic compounds (32). However, the impact of PAHs on marsh sediment microbial communities, as well as to marsh plants and the ecosystem at large, are still being realized (see, for example, references 35,77,78).…”

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confidence: 99%

Salt Marsh Bacterial Communities before and after the Deepwater Horizon Oil Spill

Engel

Liu

Paterson

et al. 2017

Appl Environ Microbiol

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Coastal salt marshes along the northern Gulf of Mexico shoreline received varied types and amounts of weathered oil residues after the 2010 Deepwater Horizon oil spill. At the time, predicting how marsh bacterial communities would respond and/or recover to oiling and other environmental stressors was difficult because baseline information on community composition and dynamics was generally unavailable. Here, we evaluated marsh vegetation, physicochemistry, flooding frequency, hydrocarbon chemistry, and subtidal sediment bacterial communities from 16S rRNA gene surveys at 11 sites in southern Louisiana before the oil spill and resampled the same marshes three to four times over 38 months after the spill. Calculated hydrocarbon biomarker indices indicated that oil replaced native natural organic matter (NOM) originating from Spartina alterniflora and marine phytoplankton in the marshes between May 2010 and September 2010. At all the studied marshes, the major class-and order-level shifts among the phyla Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria occurred within these first 4 months, but another community shift occurred at the time of peak oiling in 2011. Two years later, hydrocarbon levels decreased and bacterial communities became more diverse, being dominated by Alphaproteobacteria (Rhizobiales), Chloroflexi (Dehalococcoidia), and Planctomycetes. Compositional changes through time could be explained by NOM source differences, perhaps due to vegetation changes, as well as marsh flooding and salinity excursions linked to freshwater diversions. These findings indicate that persistent hydrocarbon exposure alone did not explain long-term community shifts. IMPORTANCE Significant deterioration of coastal salt marshes in Louisiana has been linked to natural and anthropogenic stressors that can adversely affect how ecosystems function. Although microorganisms carry out and regulate most biogeochemical reactions, the diversity of bacterial communities in coastal marshes is poorly known, with limited investigation of potential changes in bacterial communities in response to various environmental stressors. The Deepwater Horizon oil spill provided an unprecedented opportunity to study the long-term effects of an oil spill on microbial systems in marshes. Compared to previous studies, the significance of our research stems from (i) a broader geographic range of studied marshes, (ii) an extended time frame of data collection that includes prespill conditions, (iii) a more accurate procedure using biomarker indices to understand oiling, and (iv) an examination of other potential stressors linked to in situ environmental changes, aside from oil exposure.

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Characterization of the indigenous PAH-degrading bacteria of Spartina dominated salt marshes in the New York/New Jersey Harbor

Cited by 20 publications

References 37 publications

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Advances in the field of high‐molecular‐weight polycyclic aromatic hydrocarbon biodegradation by bacteria

Salt Marsh Bacterial Communities before and after the Deepwater Horizon Oil Spill

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