Characterization of the Jumbo Squid (Dosidicus gigas) Skin By-Product by Shotgun Proteomics and Protein-Based Bioinformatics

Carrera, Mónica; Ezquerra‐Brauer, Josafat Marina; Aubourg, Santiago P.

doi:10.3390/md18010031

Cited by 26 publications

(23 citation statements)

References 50 publications

(56 reference statements)

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“…In Peptide Ranker analysis, peptides with scores higher than 0.9 were considered to have more biological potential activity ( 38 ). Peptide 3–phosphokinase–1–carboxyvinyltransferase’s in amino acid metabolism ranker scores reached 0.971099 and 0.998508.…”

Section: Resultsmentioning

confidence: 99%

Bioinformatic Prediction and Characterization of Proteins in Porphyra dentata by Shotgun Proteomics

Yang

et al. 2022

Front. Nutr.

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Porphyra dentata is an edible red seaweed with high nutritional value. It is widely cultivated and consumed in East Asia and has vast economic benefits. Studies have found that P. dentata is rich in bioactive substances and is a potential natural resource. In this study, label-free shotgun proteomics was first applied to identify and characterize different harvest proteins in P. dentata. A total of 13,046 different peptides were identified and 419 co-expression target proteins were characterized. Bioinformatics was used to study protein characteristics, functional expression, and interaction of two important functional annotations, amino acid, and carbohydrate metabolism. Potential bioactive peptides, protein structure, and potential ligand conformations were predicted, and the results suggest that bioactive peptides may be utilized as high-quality active fermentation substances and potential targets for drug production. Our research integrated the global protein database, the first time bioinformatic analysis of the P. dentata proteome during different harvest periods, improves the information database construction and provides a framework for future research based on a comprehensive understanding.

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Section: Resultsmentioning

confidence: 99%

Bioinformatic Prediction and Characterization of Proteins in Porphyra dentata by Shotgun Proteomics

Yang

et al. 2022

Front. Nutr.

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“…In gel-free approaches, also known as shotgun proteomics, the proteins are directly digested in the extract with a selected enzyme, and the obtained mixture of peptides is subsequently analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) [15,16]. It is possible to perform multidimensional LC separations, combining, for example, strong anion/cation exchange chromatography and reverse-phase chromatography [17].…”

Section: Workflow Of Proteomics: Discovery and Targeted Proteomicsmentioning

confidence: 99%

Mass Spectrometry in Food Analysis

Nollet¹,

Winkler²

2022

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Aquaculture is the breeding of aquatic organisms under controlled conditions, involving both marine and freshwater fish along with algae, crustaceans, and mollusks. According to the Food and Agriculture Organization of the United Nations (FAO), this food sector represents a significant source of nutrients for the human diet and produces approximately 97.2 million tons of fish annually, which represents 47% of the global fish production [1]. With 9 billion people expected to be living on the planet by 2050, maintaining the current level of fish consumption (9.0-20.2 kg annually per capita) is a challenging task [1]. Aquaculture, the most rapidly growing food-producing sector in CONTENTS 4.

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“…In gel-free approaches, also known as shotgun proteomics, the proteins are directly digested in the extract with a selected enzyme, and the obtained mixture of peptides is subsequently analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (LC-MS/MS) [15,16]. It is possible to perform multidimensional LC separations, combining, for example, strong anion/cation exchange chromatography (SA/CX) and reverse phase (RP) chromatography [17].…”

Section: Workflow Of Proteomics: Discovery and Targeted Proteomicsmentioning

confidence: 99%

Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products

Carrera

Piñeiro

Martı́nez

2020

Foods

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This review presents the primary applications of various proteomic strategies to evaluate the impact of farming conditions on food quality and safety in aquaculture products. Aquaculture is a quickly growing sector that represents 47% of total fish production. Food quality, dietary management, fish welfare, the stress response, food safety, and antibiotic resistance, which are covered by this review, are among the primary topics in which proteomic techniques and strategies are being successfully applied. The review concludes by outlining future directions and potential perspectives.

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Characterization of the Jumbo Squid (Dosidicus gigas) Skin By-Product by Shotgun Proteomics and Protein-Based Bioinformatics

Cited by 26 publications

References 50 publications

Bioinformatic Prediction and Characterization of Proteins in Porphyra dentata by Shotgun Proteomics

Bioinformatic Prediction and Characterization of Proteins in Porphyra dentata by Shotgun Proteomics

Mass Spectrometry in Food Analysis

Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products

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