Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear

Park, Jungkum; Lee, Gyu Min; Kim, Donghyuk; Park, Duck Hwan; Oh, Chang‐Sik

doi:10.5423/ppj.nt.06.2018.0100

Cited by 39 publications

(27 citation statements)

References 26 publications

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“…It exhibited high host specificity against the E. pyrifoliae strains ( Table 1 ) compared to the myophages or podophages that were isolated from South Korea. Nevertheless, pEp_SNUABM_08 may possess biocontrol and diagnostic potential for black shoot disease, which has been previously reported in Gangwon province, South Korea [ 23 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 93%

The Bacteriophage pEp_SNUABM_08 Is a Novel Singleton Siphovirus with High Host Specificity for Erwinia pyrifoliae

Kim

Roh

Park

et al. 2021

Viruses

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Species belonging to the genus Erwinia are predominantly plant pathogens. A number of bacteriophages capable of infecting Erwinia have been used for the control of plant diseases such as fire blight. Public repositories provide the complete genome information for such phages, which includes genomes ranging from 30 kb to 350 kb in size. However, limited information is available regarding bacteriophages belonging to the family Siphoviridae. A novel lytic siphophage, pEp_SNUABM_08, which specifically infects Erwinia pyrifoliae, was isolated from the soil of an affected apple orchard in South Korea. A comprehensive genome analysis was performed using the Erwinia-infecting siphophage. The whole genome of pEp_SNUABM_08 comprised 62,784 bp (GC content, 57.24%) with 79 open reading frames. The genomic characteristics confirmed that pEp_SNUABM_08 is a singleton lytic bacteriophage belonging to the family Siphoviridae, and no closely related phages have been reported thus far. Our study not only characterized a unique phage, but also provides insight into the genetic diversity of Erwinia bacteriophages.

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Section: Discussionmentioning

confidence: 93%

The Bacteriophage pEp_SNUABM_08 Is a Novel Singleton Siphovirus with High Host Specificity for Erwinia pyrifoliae

Kim

Roh

Park

et al. 2021

Viruses

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“…A bacteriophage phiEaP‐8 was isolated from apple orchard soil and was shown to control efficiently and specifically E . amylovora (Park et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. A bacteriophage phiEaP-8 was isolated from apple orchard soil and was shown to control efficiently and specifically E. amylovora (Park et al, 2018).…”

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confidence: 99%

Characterization of the biocontrol activity of three bacterial isolates against the phytopathogen Erwinia amylovora

et al. 2021

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Antibiotics are sprayed on apple and pear orchards to control, among other pathogens, the bacterium Erwinia amylovora , the causative agent of fire blight. As with many other pathogens, we observe the emergence of antibiotic‐resistant strains of E . amylovora . Consequently, growers are looking for alternative solutions to combat fire blight. To find alternatives to antibiotics against this pathogen, we have previously isolated three bacterial strains with antagonistic and extracellular activity against E . amylovora , both in vitro and in planta, corresponding to three different bacterial genera: Here, we identified the inhibitory mode of action of each of the three isolates against E . amylovora . Isolate Bacillus amyloliquefaciens subsp. plantarum (now B . velezensis ) FL50S produces several secondary metabolites including surfactins, iturins, and fengycins. Specifically, we identified oxydifficidin as the most active against E . amylovora S435. Pseudomonas poae FL10F produces an active extracellular compound against E . amylovora S435 that can be attributed to white‐line‐inducing principle (WLIP), a cyclic lipopeptide belonging to the viscosin subfamily (massetolide E, F, L, or viscosin). Pantoea agglomerans NY60 has a direct cell‐to‐cell antagonistic effect against E . amylovora S435. By screening mutants of this strain generated by random transposon insertion with decreased antagonist activity against strain S435, we identified several defective transposants. Of particular interest was a mutant in a gene coding for a Major Facilitator Superfamily (MFS) transporter corresponding to a transmembrane protein predicted to be involved in the extracytoplasmic localization of griseoluteic acid, an intermediate in the biosynthesis of the broad‐spectrum phenazine antibiotic d ‐alanylgriseoluteic acid.

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“…E. amylovora phages vary in their host specificity, with some specific to individual strains and others infecting additional species such as Pantoea spp. (Nagy et al 2012;Park et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Characterization ofErwinia tracheiphilaBacteriophage FBB1 Isolated from Spotted Cucumber Beetles that VectorE. tracheiphila

Zhai

Beattie

2021

Phytopathology®

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Erwinia tracheiphila, the causal pathogen of bacterial wilt of cucurbit crops, is disseminated by cucumber beetles. A bacteriophage, designated FBB1, was isolated from spotted cucumber beetles (Diabrotica undecimpunctata) that were collected from a field where E. tracheiphila is endemic. FBB1 was classified into the Myoviridae family based on its morphology, which includes an elongated icosahedral head (106 × 82 nm) and a putatively contractile tail (120 nm). FBB1 infected all 62 E. tracheiphila strains examined and also three Pantoea spp. strains. FBB1 virions were stable at 55°C for 1 h and tolerated a pH range from 3 to 12. FBB1 has a genome of 175,994 bp with 316 predicted coding sequences and a GC content of 36.5%. The genome contains genes for a major bacterial outer-membrane protein, a putative exopolysaccharide depolymerase, and 22 predicted tRNAs. The morphology and genome indicate that FBB1 is a T4-like virus and thus in the Tevenvirinae subfamily. FBB1 is the first virulent phage of E. tracheiphila to be reported, and to date, is one of only two bacteriophages to be isolated from insect vectors of phytopathogens. Collectively, the results support FBB1 as a promising candidate for biocontrol of E. tracheiphila based on its virulent (lytic) rather than lysogenic lifestyle, its infection of all E. tracheiphila strains examined to date, and its infection of a few non-pathogenic bacteria that could be used to support phage populations when pathogen numbers are low.

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Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear

Cited by 39 publications

References 26 publications

The Bacteriophage pEp_SNUABM_08 Is a Novel Singleton Siphovirus with High Host Specificity for Erwinia pyrifoliae

The Bacteriophage pEp_SNUABM_08 Is a Novel Singleton Siphovirus with High Host Specificity for Erwinia pyrifoliae

Characterization of the biocontrol activity of three bacterial isolates against the phytopathogen Erwinia amylovora

Characterization ofErwinia tracheiphilaBacteriophage FBB1 Isolated from Spotted Cucumber Beetles that VectorE. tracheiphila

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