Characterization of the murine CTP:phosphocholine cytidylyltransferase beta gene promoter

PCYT1A Regulates Phosphatidylcholine Homeostasis from the Inner Nuclear Membrane in Response to Membrane Stored Curvature Elastic Stress

“…In most cell types, there is a second cytosolic PCYT1B isoform that presumably senses changes in the ER and possibly other membrane bound organelles. However, its expression appears to be very low in many cell types ( Marcucci et al., 2008 , Karim et al., 2003 ), and Pcyt1b null mice manifest subtle phenotypes including axonal branching defects in sympathetic neurons and reproductive insufficiency mainly resulting due to defective ovarian follicles and spermatogenesis ( Strakova et al., 2011 , Jackowski et al., 2004 ), whereas Pcyt1a null mice die in utero ( Wang et al., 2005 ). The fact that the nuclear envelope is continuous with the ER could presumably facilitate PL diffusion from within the nucleus to the peripheral ER and vice versa, which might also account for our observations suggesting that NLS mutants of Pct1 in yeast and CCT1 in flies can compensate for the absence of WT enzyme.…”

CTP:phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis

“…Murine Pcyt1b transcription was induced during neurite growth [92,93] and H-ras transformation [155]. While the promoter elements of the murine CCTb gene have been mapped in silico and by reporter analysis, physiologically relevant transcription factors have not been identified [158]. CCT mRNA expression in the vascular plant P. sativum was increased by the hormone indole acetic acid (IAA) [86], however IAA treatment reduced the cellular CCT activity and PC synthesis rates [159].…”

Role of Phosphatidylcholine during Neuronal differentiation

“…CCT is encoded by two genes named Pcyt1a and Pcyt1b ; the first expresses alternative transcripts termed CCTα1 and CCTα2 that, when translated, produce the same protein called CCTα (22). Pcyt1b drives the expression of CCTβ2 and CCTβ3 proteins, which are derived from alternative promoters and differentially spliced transcripts (16, 22, 23). CCTα is ubiquitously expressed in nucleated cells, whereas CCTβ is abundant in brain and gonads (22).…”