Characterization of the nasopharyngeal and middle ear microbiota in gastroesophageal reflux-prone versus gastroesophageal reflux non-prone children

Boers, Stefan A.; Zeeuw, Marjolein de; Jansen, Ruud; Schroeff, Marc P. van der; Rossum, Annemarie M. C. van; Hays, John P.; Verhaegh, Suzanne J. C.

doi:10.1007/s10096-017-3178-2

Cited by 15 publications

(16 citation statements)

References 38 publications

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“…Staphylococcus -dominated airway microbiomes has also been associated with an increased risk of bronchiolitis (Hasegawa et al, 2017). In contrast, Turicella otiditis and Alloiococcus otitis have been isolated almost exclusively from the human ear and have frequently been attributed to OM (Tano et al, 2008; von Graevenitz and Funke, 2014; Boers et al, 2018). The absence of these species from other parts of the upper respiratory tract has led to a debate about whether they are OM pathogens or part of commensal microbiota.…”

Section: Discussionmentioning

confidence: 99%

Altered Middle Ear Microbiome in Children With Chronic Otitis Media With Effusion and Respiratory Illnesses

Kolbe

Castro-Nallar

Preciado

et al. 2019

Front. Cell. Infect. Microbiol.

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Chronic otitis media with effusion (COME) is a common childhood disease characterized by an accumulation of fluid behind the eardrum. COME often requires surgical intervention and can also lead to significant hearing loss and subsequent learning disabilities. Recent characterization of the middle ear fluid (MEF) microbiome in pediatric patients has led to an improved understanding of the microbiota present in the middle ear during COME. However, it is not currently known how the MEF microbiome might vary due to other conditions, particularly respiratory disorders. Here, we apply an amplicon sequence variant (ASV) pipeline to MEF 16S rRNA high-throughput sequencing data from 50 children with COME (ages 3–176 months) undergoing tube placement. We achieve a more detailed taxonomic resolution than previously reported, including species and genus level resolution. Additionally, we provide the first report of the functional roles of the MEF microbiome and demonstrate that despite high taxonomic diversity, the functional capacity of the MEF microbiome remains uniform between patients. Furthermore, we analyze microbiome differences between children with COME with and without a history of lower airway disease (i.e., asthma or bronchiolitis). The MEF microbiome was less diverse in participants with lower airway disease than in patients without, and phylogenetic β-diversity (weighted UniFrac) was significantly different based on lower airway disease status. Differential abundance between patients with lower airway disease and those without was observed for the genera Haemophilus, Moraxella, Staphylococcus, Alloiococcus, and Turicella. These findings support previous suggestions of a link between COME and respiratory illnesses and emphasize the need for future study of the middle ear and respiratory tract microbiomes in diseases such as asthma and bronchiolitis.

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Section: Discussionmentioning

confidence: 99%

Altered Middle Ear Microbiome in Children With Chronic Otitis Media With Effusion and Respiratory Illnesses

Kolbe

Castro-Nallar

Preciado

et al. 2019

Front. Cell. Infect. Microbiol.

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“…The role of the respiratory tract microbiome in pathogenesis and immune modulation of AOM remain unexplored. Middle ear (ME) microbiome has been reported in chronic otitis media (Santos-Cortez et al, 2016; Krueger et al, 2017; Boers et al, 2018; Johnston et al, 2019), and NP microbiome is associated with pathogenesis of upper respiratory traction infection and AOM (Lappan et al, 2018). None of these studies investigate changes in NP microbiota during onset of AOM.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Microbiome in Nasopharynx and Middle Ear in Young Children With Acute Otitis Media

Gill

et al. 2019

Front. Genet.

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Acute otitis media (AOM) is the most common pediatric infection for which antibiotics are prescribed in the United States. The role of the respiratory tract microbiome in pathogenesis and immune modulation of AOM remains unexplored. We sought to compare the nasopharyngeal (NP) microbiome of children 1 to 3 weeks prior to onset of AOM vs. at onset of AOM, and the NP microbiome with the microbiome in middle ear (ME). Six children age 6 to 24 months old were studied. Nasal washes (NW) were collected at healthy visits 1 to 3 weeks prior to AOM and at onset of AOM. The middle ear fluids (MEF) were collected by tympanocentesis at onset of AOM. Samples were stored in Trizol reagents or phosphate-buffered saline (PBS) at −80°C until use. The microbiome was characterized by 16S rRNA gene sequencing. Taxonomic designations and relative abundance of bacteria were determined using the RDP classifier tool through QIIME. Cumulative sum scaling normalization was applied before determining bacterial diversity and abundance. Shannon diversity index was calculated in Microsoft excel. The relative abundance of each bacteria species was compared via Mann-Whitney U test. We found that the NW microbiome of children during healthy state or at baseline was more diverse than microbiome during AOM. At AOM, no significant difference in microbiome diversity was found between NW and MEF, although some bacteria species appear to differ in MEF than in NW. The microbiome of samples stored in PBS had significant greater diversity than samples stored in Trizol reagent.

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“…as the most abundant taxa in MEF while absent in NP samples. As for GER, no apparent effects were found on the NP and ME microbiota in the two groups [75].…”

Section: Otitis Media With Effusionmentioning

confidence: 80%

Upper Respiratory Tract Microbiome and Otitis Media Intertalk: Lessons from the Literature

Folino

Ruggiero

Capaccio

et al. 2020

JCM

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Otitis media (OM) is one of the most common diseases occurring during childhood. Microbiological investigations concerning this topic have been primarily focused on the four classical otopathogens (Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pyogenes) mainly because most of the studies have been conducted with culture-dependent methods. In recent years, the introduction of culture-independent techniques has allowed high-throughput investigation of entire bacterial communities, leading to a better comprehension of the role of resident flora in health and disease. The upper respiratory tract (URT) is a region of major interest in otitis media pathogenesis, as it could serve as a source of pathogens for the middle ear (ME). Studies conducted with culture-independent methods in the URT and ME have provided novel insights on the pathogenesis of middle ear diseases through the identification of both possible new causative agents and of potential protective bacteria, showing that imbalances in bacterial communities could influence the natural history of otitis media in children. The aim of this review is to examine available evidence in microbiome research and otitis media in the pediatric age, with a focus on its different phenotypes: acute otitis media, otitis media with effusion and chronic suppurative otitis media.

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Characterization of the nasopharyngeal and middle ear microbiota in gastroesophageal reflux-prone versus gastroesophageal reflux non-prone children

Cited by 15 publications

References 38 publications

Altered Middle Ear Microbiome in Children With Chronic Otitis Media With Effusion and Respiratory Illnesses

Altered Middle Ear Microbiome in Children With Chronic Otitis Media With Effusion and Respiratory Illnesses

Comparative Analysis of Microbiome in Nasopharynx and Middle Ear in Young Children With Acute Otitis Media

Upper Respiratory Tract Microbiome and Otitis Media Intertalk: Lessons from the Literature

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