Characterization of the neuropeptide Y system in the frog Silurana tropicalis (Pipidae): Three peptides and six receptor subtypes

Sundström, Görel; Xu, Bo; Larsson, Tomas; Heldin, Johan; Bergqvist, Christina; Fredriksson, Robert; Conlon, J. Michael; Lundell, Ingrid; Denver, RJ; Larhammar, Dan

doi:10.1016/j.ygcen.2012.04.027

Cited by 17 publications

(9 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Y1 gene is the only member of the NPY receptor family that has an intron in the coding region. This intron is very small in mammals (97 bp in the human gene, 110 bp in opossum), chicken (121 bp), anole lizard (698 bp), and the frog Silurana tropicalis (92 bp; Sundstrom et al, 2012), but considerably larger in zebrafish with approximately 40 kb (in preparation), a sturgeon (Salaneck et al, 2008), and the two cartilaginous fishes spiny dogfish (Salaneck et al, 2003) and elephant shark (Larsson et al, 2009), the last-mentioned having an intron >3 kb. In the coelacanth, this intron is approximately 2.5 kb (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

“…All of the other gnathostome lineages seem to have suffered losses, although some of the genome databases may be incomplete. Among amphibians, the western clawed frog Silurana (Xenopus) tropicalis seems to have lost Y6 which appears to be a pseudogene in the frog Pelophylax esculentus (previously called Rana esculenta ; Sundstrom et al, 2012). Amniotes have lost Y8, or possibly this gene was lost independently in birds and mammals (Larsson et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ancient Grandeur of the Vertebrate Neuropeptide Y System Shown by the Coelacanth Latimeria chalumnae

Larhammar¹,

Bergqvist²

2013

Front. Neurosci.

View full text Add to dashboard Cite

The neuropeptide Y (NPY) family receptors and peptides have previously been characterized in several tetrapods, teleost fishes, and in a holocephalan cartilaginous fish. This has shown that the ancestral NPY system in the jawed vertebrates consisted of the peptides NPY and peptide YY (PYY) and seven G-protein-coupled receptors named Y1–Y8 (Y3 does not exist). The different vertebrate lineages have subsequently lost or gained a few receptor genes. For instance, the human genome has lost three of the seven receptors while the zebrafish has lost two and gained two receptor genes. Here we describe the NPY system of a representative of an early diverging lineage among the sarcopterygians, the West Indian Ocean coelacanth Latimeria chalumnae. The coelacanth was found to have retained all seven receptors from the ancestral jawed vertebrate. The receptors display the typical characteristics found in other vertebrates. Interestingly, the coelacanth was found to have the local duplicate of the PYY gene, called pancreatic polypeptide, previously only identified in tetrapods. Thus, this duplication took place very early in the sarcopterygian lineage, before the origin of tetrapods. These findings confirm the ancient complexity of the NPY system and show that mammals have lost more NPY receptors than any other vertebrate lineage. The coelacanth has all three peptides found in tetrapods and has retained the ancestral jawed vertebrate receptor repertoire with neither gains or losses.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ancient Grandeur of the Vertebrate Neuropeptide Y System Shown by the Coelacanth Latimeria chalumnae

Larhammar¹,

Bergqvist²

2013

Front. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Subsequently, gene losses and a few additional gene duplications have taken place in some of the lineages: mammals and birds have lost Y8 independently, mammals have lost Y7, and a functional Y6 receptor has been lost independently in some mammals, lizards, frogs and teleost fishes, whereas teleost fish have acquired a duplicate of Y2 named Y2-2 and a duplicate of Y8 named Y8b (Starbäck et al, 2000;Larsson et al, 2008Larsson et al, , 2009Fallmar et al, 2011;Sundstrom et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Another difference between the subtypes in zebrafish is that all three peptides have higher affinity for Y4 than for the other receptors . In the western clawed frog, only three of the six receptors were possible to express in cell culture (Sundstrom et al, 2012). Among these Y8 had higher affinity for all three native peptides (NPY, PYY and PP) than either Y5 or Y7.…”

Section: Introductionmentioning

confidence: 99%

Neuropeptide Y family receptors Y1 and Y2 from sea lamprey, Petromyzon marinus

Lagman

Sundström

et al. 2015

General and Comparative Endocrinology

View full text Add to dashboard Cite

“…In general, this receptor has a broad distribution, with the highest expression in the hypothalamus, thalamus, and several rhombencephalic nuclei (Nichol et al, ; Parker and Herzog, ; Durkin et al, ; Wolak et al, 2003; Holmberg et al, ; Fetissov et al, ; Morin and Gehlert, ). Furthermore, the Y5 receptor has been cloned and pharmacologically characterized in a number of nonmammalian species, such as Gallus gallus (Holmberg et al, ), Latimeria chalumnae (Larsson et al, ), Squalus acanthias , Polypterus senegalus , Acipenser baerii , Amia calva , Osteoglossum bicirrhosum (Salaneck et al, ), Callorhinchus milii (Larsson et al, ), and Silurana tropicalis (Sundström et al, ), but it was not found in the euteleosts pufferfish ( Takifugu rubripes ) and zebrafish ( Danio rerio ) (Larsson et al, ). However, studies on the distribution of the Y5 receptor in nonmammalian vertebrates are practically nonexistent.…”

mentioning

confidence: 99%

Cloning, phylogeny, and regional expression of a Y5 receptor mRNA in the brain of the sea lamprey (Petromyzon marinus)

Pérez‐Fernández

Megı́as

Pombal

2014

J of Comparative Neurology

View full text Add to dashboard Cite

The NPY receptors known as Y receptors are classified into three subfamilies, Y1, Y2, and Y5, and are involved in different physiological functions. The Y5 receptor is the only member of the Y5 subfamily, and it is present in all vertebrate groups, except for teleosts. Both molecular and pharmacological studies show that Y5 receptor is highly conserved during vertebrate evolution. Furthermore, this receptor is widely expressed in the mammalian brain, including the hypothalamus, where it is thought to take part in feeding and homeostasis regulation. Lampreys belong to the agnathan lineage, and they are thought to have branched out between the two whole-genome duplications that occurred in vertebrates. Therefore, they are in a key position for studies on the evolution of gene families in vertebrates. Here we report the cloning, phylogeny, and brain expression pattern of the sea lamprey Y5 receptor. In phylogenetic studies, the lamprey Y5 receptor clusters in a basal position, together with Y5 receptors of other vertebrates. The mRNA of this receptor is broadly expressed in the lamprey brain, being especially abundant in hypothalamic areas. Its expression pattern is roughly similar to that reported for other vertebrates and parallels the expression pattern of the Y1 receptor subtype previously described by our group, as it occurs in mammals. Altogether, these results confirm that a Y5 receptor is present in lampreys, thus being highly conserved during the evolution of vertebrates, and suggest that it is involved in many brain functions, the only known exception being teleosts.

show abstract

Characterization of the neuropeptide Y system in the frog Silurana tropicalis (Pipidae): Three peptides and six receptor subtypes

Cited by 17 publications

References 49 publications

Ancient Grandeur of the Vertebrate Neuropeptide Y System Shown by the Coelacanth Latimeria chalumnae

Ancient Grandeur of the Vertebrate Neuropeptide Y System Shown by the Coelacanth Latimeria chalumnae

Neuropeptide Y family receptors Y1 and Y2 from sea lamprey, Petromyzon marinus

Cloning, phylogeny, and regional expression of a Y5 receptor mRNA in the brain of the sea lamprey (Petromyzon marinus)

Contact Info

Product

Resources

About