Characterization of the phenolic compound, gallic acid from Sansevieria roxburghiana schult and schult. f. rhizomes and antioxidant and cytotoxic activities evaluation

Abstract

Background:Sansevieria roxburghiana Schult. and Schult. f. (Asparagaceae) grows in India, Indonesia, Sri Lanka, and tropical Africa. Even though the plant has been traditionally used for the treatment of many ailments, the antioxidant and antiproliferative activities of S. roxburghiana methanol extract and its fractions have not yet been explored.Materials and Methods:Quantitative estimation of phenols and different antioxidant assays were performed using standard methods. Anti-proliferative effect of the extract and fractions were evaluated in HCT-116, HeLa, MCF-7, HepG2, and A-549 cancer cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sulforhodamine B (SRB) assay methods. High-performance liquid chromatography (HPLC) and high-performance thin layer chromatography (HPTLC) fingerprint profiling were carried out for extract and different fractions.Results:Significant antioxidant and anti-proliferate activity were detected in ethyl acetate fraction. Ethyl acetate fraction showed prominent scavenging activity in 1,1-diphenyl-2-picrylhydrazyl, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and nitric oxide antioxidant assays with an concentration yielding 50% inhibition (IC50) 15.33 ± 1.45, 45.3 ± 1.93 and 48.43 ± 0.46 mg/ml, respectively. Cytotoxicity of ethyl acetate fraction was the highest among other fractions against HCT-116, HeLa, and MCF-7cancer cell lines with IC50 values 16.55 ± 1.28, 12.38 ± 1.36, and 8.03 ± 1.9 μg/ml, respectively, by MTT assay and 15.57 ± 0.70, 13.19 ± 0.49, and 10.34 ± 0.9 μg/ml, respectively, by SRB assay. The presence of gallic acid in the ethyl acetate fraction of S. roxburghiana rhizomes was confirmed by HPLC and HPTLC analysis.Conclusion:Results suggested that ethyl acetate fraction exhibited effective antioxidant and antiproliferative activities. The phenolic compounds identified in ethyl acetate fraction could be responsible for the activities.SUMMARY Sansevieria roxburghiana has been selected for in vitro antioxidant and cytotoxicity screeningEthyl acetate fraction of methanol extract of S. roxburghiana exhibited effective antioxidant and antiproliferative activitiesThe activity of ethyl acetate fraction may be due to the presence of phenolic compound which is identified by high-performance liquid chromatography and high-performance thin layer chromatography techniques. Abbreviations used: %: Percent, ºC: Celsius, mg: Microgram, ml-Microlitre, ANOVA: Analysis of variance, DMSO: Dimethyl sulfoxide, g: Grams, IC50: Concentration yielding 50% inhibition, Kg: Kilogram, mg: Milligram, min: Minutes, ml: Milliliter, HPLC: High-performance liquid chromatography, HPTLC: High-performance thin layer chromatography, DPPH: 1,1-diphenyl-2-picrylhydrazyl, ABTS: 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, GAE: Gallic acid equivalents, SRME: Methanol extract of S. roxburghiana, ROS: Reactive oxygen species, SRPE: Petroleum ether fraction o...

Polyphenols Extracts From Didymosphenia Geminata (Lyngbye) Schmidt Altered the Motility and Viability of Daphnia Magna

“…The toxicity biotests are carried out by checking organisms parameters (Walker et al 2012). We recorded whether this effect occurred when river water contaminated with D. geminata, a standard sample of polyphenols and acid gallic, was used (Maheshwari et al 2017), acutely and chronically. Figure 3 shows a reduction on the D. magna motility in all the conditions; likewise, the acid gallic had a stronger effect on the motility, supporting the idea that the polyphenols had an effect on this parameter.…”

Polyphenols extracts from Didymosphenia geminata (Lyngbye) Schmidt altered the motility and viability of Daphnia magna

“…The toxicity biotests are carried out by checking organisms parameters (Walker et al 2012). We recorded whether this effect occurred when river water contaminated with D. geminata, a standard sample of polyphenols and acid gallic, was used (Maheshwari et al 2017), acutely and chronically. Figure 3 shows a reduction on the D. magna motility in all the conditions; likewise, the acid gallic had a stronger effect on the motility, supporting the idea that the polyphenols had an effect on this parameter.…”