1993
DOI: 10.1128/jb.175.16.5043-5048.1993
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Characterization of the RNase P RNA of Sulfolobus acidocaldarius

Abstract: RNase P is the ribonucleoprotein enzyme that cleaves precursor sequences from the 5' ends of pre-tRNAs. In Bacteria, the RNA subunit is the catalytic moiety. Eucaryal and archaeal RNase P activities copurify with RNAs, which have not been shown to be catalytic. We report here the analysis of the RNase P RNA from the thermoacidophilic archaeon Sudfolobus ocanarius. The holoenzyme was highly purified, and extracted RNA was used to identify the RNase P RNA gene. The nucleotide sequence of the gene was determined,… Show more

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Cited by 39 publications
(23 citation statements)
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“…Full or partial genes for RNase P RNAs also have been isolated from many archaeal species, including representatives of the two main phylogenetic branches of Archaea: Crenarchaeota and Euryarchaeota (e.g. 17,33,34). In contrast, all known eucaryal RNase P RNA sequences are from either yeasts (e.g.…”
Section: Obtaining Sequencesmentioning
confidence: 99%
See 1 more Smart Citation
“…Full or partial genes for RNase P RNAs also have been isolated from many archaeal species, including representatives of the two main phylogenetic branches of Archaea: Crenarchaeota and Euryarchaeota (e.g. 17,33,34). In contrast, all known eucaryal RNase P RNA sequences are from either yeasts (e.g.…”
Section: Obtaining Sequencesmentioning
confidence: 99%
“…Genes encoding the RNA subunits of RNase P have been identified by two basic approaches: (a) genetic screens for factors required for pre-tRNA processing (8,15,16) and (b) hybridization of RNAs extracted from highly purified RNase P to genomic DNA libraries (17)(18)(19)(20)(21)(22)(23)(24). Once identified, RNase P RNA sequences can be used to isolate homologous RNAs from other organisms by either heterologous probing under conditions for low-stringency hybridization (25,26), inspection of genomic sequence databases (27)(28)(29), or polymerase chain reaction (PCR) amplification (30,31).…”
Section: Obtaining Sequencesmentioning
confidence: 99%
“…The S. acidocaldarius RNase P is resistant to micrococcal nuclease treatment and contains a 315-nt RNA that persists after nuclease treatment (9). The enzyme is large (Ϸ400 kDa apparent molecular mass) and has a low density in Cs 2 SO 4 (1.27 g͞cm 3 ), similar to the densities of the eukaryotic nuclear enzyme and implying a high protein:RNA content.…”
mentioning
confidence: 99%
“…The RNA component of eubacterial RNase P has catalytic activity in vitro in the absence of protein cofactors. In contrast, the RNA components of archaebacterial (Nieuwlandt et al 1991;LaGrandeur et al 1993) and eukaryotic RNase P have not been found to be catalytic in the absence of proteins, despite their considerable structural homology to the eubacterial RNAs. E. coli RNase P contains a single protein, designated C5, which is small (119 amino acids, 13.8 kD) and highly basic.…”
mentioning
confidence: 99%