Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells

Epps, Dennis E. Van; Wolfe, Mark L.; Groppi, Vincent E.

doi:10.1016/0009-3084(94)90035-3

Cited by 182 publications

(134 citation statements)

References 18 publications

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“…1b). Viable cells are able to exclude the anionic DiBAC 4 (3) dye, due to the presence of a transmembrane potential; on the contrary, the fluorescent probe can freely enter in the cells with depolarized membrane, where it binds to intracellular proteins or membranes and enhances significantly the green fluorescence (Epps et al 1994;Dinsdale et al 1995). Similar results were observed, by epifluorescence microscopy, when PI was used (Fig.…”

Section: Resultssupporting

confidence: 75%

Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae

Bussche

Soares

2010

Appl Microbiol Biotechnol

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In the present work, the mode of cell death induced by Pb in Saccharomyces cerevisiae was studied. Yeast cells Pb-exposed, up to 6 h, loss progressively the capacity to proliferate and maintained the membrane integrity evaluated by the fluorescent probes bis(1,3-dibutylbarbituric acid trimethine oxonol) and propidium iodide. Pb-induced death is an active process, requiring the participation of cellular metabolism, since the simultaneous addition of cycloheximide attenuated the loss of cell proliferation capacity. Cells exposed to Pb accumulated intracelullarly reactive oxygen species (ROS), evaluated by 2′,7′-dichlorodihydrofluorescein diacetate. The addition of ascorbic acid (a ROS scavenger) strongly reduced the oxidative stress and impaired the loss of proliferation capacity in Pb-treated cells. Pb-exposed cells displayed nuclear morphological alterations, like chromatin fragmentation, as revealed by diaminophenylindole staining. Together, the data obtained indicate that yeast cells exposition to 1 mmol/l Pb results in severe oxidative stress which can be the trigger of programmed cell death by apoptosis.

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Section: Resultssupporting

confidence: 75%

Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae

Bussche

Soares

2010

Appl Microbiol Biotechnol

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“…The b-TC3 cells were maintained in DMEM containing, in addition, glutamax-1 (l-alanyl-l-glutamine 0.862 mg/ml), sodium pyruvate (110 mg/ml), pyridoxine (4 mg/ml), glucose (25 mmol/l), non-essential amino acids, fetal calf serum (15 %), penicillin (100 U/ml) and streptomycin (100 mg/ml) (Life Technologies, Merelbeke, Belgium) [19]. After washing and pre-incubation at 37°C in a modified Krebs-Ringer-HEPES buffer (in mmol/l: NaCl 140, KCl 3.6, NaH 2 PO 4 0.5, MgSO 4 0.5, NaHCO 3 2.0, CaCl 2 1.5, dglucose 10 and HEPES 10; pH 7.4), the cells were incubated in the same buffer with the addition of 4 mmol/l of the membrane-potential sensitive dye bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC 4 (3)) [20] (Molecular Probes, Eugene, Ore., USA). The plates were then placed on a programmable stage fitted to a Nikon TMD microscope equipped with a 20´NA 0.5 Fluar objective.…”

Section: Methodsmentioning

confidence: 99%

A potent diazoxide analogue activating ATP-sensitive K + channels and inhibiting insulin release

Lebrun¹,

Arkhammar

Antoine³

et al. 2000

Diabetologia

127

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“…DiBAC 4 (3) is a slow-response potential-sensitive probe that can enter depolarized cells, binds to intracellular proteins or membrane thus exhibiting enhanced fluorescence (Epps et al 1994). An increase in depolarization generates an additional influx of the anionic dye, resulting in the increase of fluorescence.…”

Section: Transmembrane Potential Evaluationmentioning

confidence: 99%

Quercetin and epigallocatechin gallate effects on the cell membranes biophysical properties correlate with their antioxidant potential

Margină¹,

Ilie²,

Manda³

et al. 2012

gpb

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Abstract. Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short-time effect of these polyphenols on membrane anisotropy and transmembrane potential of U937 monocytes and Jurkat T lymphoblasts. Results showed that quercetin and epigallocatechin gallate induced, after 20 minutes cell exposure, a dose-dependent increase of membrane anisotropy and polarization. Anisotropy increase was correlated with the reduction of lipid peroxidation. Our results could indicate that the antioxidant capacity of the tested polyphenols is due to their stabilizing effect on the cell membranes, thus contributing to cell protection in various pathologies and as adjuvant therapy in highly toxic treatment regimens.

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Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells

Cited by 182 publications

References 18 publications

Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae

Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae

A potent diazoxide analogue activating ATP-sensitive K + channels and inhibiting insulin release

Quercetin and epigallocatechin gallate effects on the cell membranes biophysical properties correlate with their antioxidant potential

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