Characterization of the thermal-tolerant mutants of Chlorella sp. with high growth rate and application in outdoor photobioreactor cultivation

Ong, Seow Chin; Kao, Chien Ya; Chiu, Sheng Yi; Tsai, Ming Ta; Lin, Chih‐Sheng

doi:10.1016/j.biortech.2009.10.007

Cited by 94 publications

(38 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This culture has been found to be comparable with the mutant derivative of Chlorella sp. reported by Ong et al (2010) and other thermotolerant algae. Chemical composition, gross energy content, and amino acid profile of dry organism revealed its suitability as feed for aquaculture cultivation.…”

Section: Introductionmentioning

confidence: 71%

See 1 more Smart Citation

High-density growth and crude protein productivity of a thermotolerant Chlorella vulgaris: production kinetics and thermodynamics

et al. 2011

View full text Add to dashboard Cite

Current study investigated the fermentative production of cell mass and crude protein using an axenic culture of the thermotolerant strain of Chlorella vulgaris grown mixotrophically in an illuminated 10-l glass bioreactor. The process was then upscaled to 1,000-l bioreactor. The organism supported maximum specific growth rate, crude protein volumetric productivity, and specific productivity of 1.2 day -1 , 2.26 g l -1 day -1 , and 0.76 g g -1 day -1 , respectively, with urea as nitrogen source. Gibbs free energy, enthalpy, and entropy values for its formation were 74.3, 56. 2 kJ mol -1 , and -59.1 J mol -1 K -1 , respectively, in both reactors and corresponded to those of thermotolerant organisms. Algal biomass grown in 10-l bioreactor contained 0.52 ± 0.03, 12.6 ± 2.0, 60.0 ± 4.5, 0.4 ± 0.02, 4.5 ± 0.2, 12 ± 0.5, and 3.81 ± 0.5% carotenoids, carbohydrates, crude protein, DNA, RNA, lipids, and total chlorophyll, respectively. Dry biomass supported good growth of fish larvae comparable with that on commercial diet.Electronic supplementary material The online version of this article (

show abstract

Section: Introductionmentioning

confidence: 71%

“…Recently, Ong et al (2010) developed two thermotolerant mutant derivatives of Chlorella species through chemical mutagenesis. These mutants could grow fast up to 42°C and produce elevated levels of lipid and fix higher CO 2 .…”

Section: Introductionmentioning

confidence: 99%

High-density growth and crude protein productivity of a thermotolerant Chlorella vulgaris: production kinetics and thermodynamics

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Natural day light and 25-30°C temperature were favorable for overall growth of C. vulgaris. Maximum specific growth rate of C. pyrenoidosa, increased uniformly with enhanced temperature, in the range 22°C to 30°C [20], and more increase of temperature resulted in a drop of specific growth rate and cells are unable to grow at temperature above 33°C [21], so the optimum temperature for C. vulgaris is 25-30°C.It was observed in our study that in the 1 st week of experiment, the growth of Chlorella under continuous illumination, was greater than the alternate light and natural day light. This is for the reason that the incidence of adequate light energy under continuous light in the 1 st week of cultivation, during cell metabolism process, therefore, C. vulgaris is able to grow speedily.…”

mentioning

confidence: 99%

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Sharma¹,

Singh²,

Sharma³

2012

J Plant Pathol Microb

View full text Add to dashboard Cite

The effects of culture conditions at different temperature and light regimes on growth and the contents of chlorophyll-a, chlorophyll-b, total carotenoids, total protein and total free amino acids of Chlorella vulgaris were determined. The growth of C. vulgaris in terms of optical density (0.42 at 670 nm), cell count (440 x 104 cells/ml) and dry weight (30.2 mg/50 ml), and the amount of chlorophyll-a (2.16%), chlorophyll-b (0.59%) and total protein, was found higher at the temperature 25-30°C and natural day light receiving through the north facing window of the growth room. Although, the amount of total carotenoids (0.440%) and free amino acids (834 µg/gm fresh weight) were found maximum in continuous light at 30-35°C, not much differences in the amount of carotenoids (0.385%) and free amino acids (822 µg/gm fresh weight) were found at 25-30°C and natural day light. The natural day light at 25-30°C was also proved proficient, as distinct banding pattern with unique polypeptides such as 15KDa, 47KDa and 50KDa, on the other hand, 23KDa, 26KDa and 36KDa appeared in all samples, these bands were not affected by light and temperature. Our results indicate that among all five culture conditions tested, the cultures kept at north facing window receiving natural day light at temperature 25-30°C, show best growth and higher contents of biochemicals that will be beneficial to use Chlorella for high nutritive purpose. In order to find out the optimum culture condition, the cultures were subjected to five different conditions of temperature and light regimes. In the growth room, light was provided by fluorescent tube lights (40 W) having 2500 Lux intensity, and were fixed at a distance of 64 cm from the cultures in constant and alternate light conditions. One set of five different culture conditions were placed in the laboratory in window, which was facing north, for providing natural day and dark periods. The intensity of natural day light was as an average 2700 Lux. The following culture conditions set in the growth room were used in the present study: Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris Rekha Growth measurement Estimation of pigmentsChlorophyll content of the samples were extracted in 90% (v/v) acetone, and chlorophyll-a, b estimated by Parson and Strickland method [14]. Carotenoid content of the samples were extracted in 80% (v/v) acetone, and estimated by Jensen method [15]. Estimation of proteinsProtein content of the samples was estimated quantitatively by Lowry [16] by dry biomass, using bovine serum albumin (BSA) as standard. Qualitatively, the proteins were estimated by the SDS-PAGE analysis, which was carried out according to Laemmli [17]. For extraction of proteins, the samples were homogenized with lysis buffer containing 0.5M Tris-HCl, 8M Urea, 5% (w/v) SDS, 20% (v/v) Glycerol and 10% (v/v) β-Mercaptoethanol; final pH 6.8 and centrifuged at 4°C for 20 min at 10,000 rpm. Protein extract was used for SDS-PAGE analysis, using a 12% polyacrylamide gel co...

show abstract

“…In greenhouses temperatures can reach up to 55 °C, resulting in maximum culture temperatures exceeding 35 °C [13]. For outdoor cultivation similar temperatures surpassing 35 °C and even 40 °C were reported [14][15][16][17][18]. Whereas temperatures below the optimum lead to a retained biomass production, temperatures above the optimum results in a steep decrease in productivity and possibly the total loss of the culture.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of New Temperature Tolerant Microalgal Strains for Biomass Production

et al. 2014

View full text Add to dashboard Cite

Microalgae exhibit great potential for biomass production. Although microalgae display an enormous biodiversity, surprisingly only 15 species are used for large scale production processes worldwide. The implementation of new production strains with good process-oriented properties, especially fast growth rate and heat resistance, could improve production efficiency and reduce costs. In this study 130 environmental samples collected in Germany, Spain, Italy and Portugal were investigated for fast growing thermotolerant photosynthetic species. Isolates were characterized and identified on a molecular level. In total 21 of the isolated freshwater strains were able to grow at 40 °C. Additionally, 13 of those 21 strains are able to grow at 45 °C. The highest growth rate at room temperature was 1.16 per day (isolate T306A), compared to 0.053 per day at 45 °C (isolate Sp13). In three thermotolerant strains pigment production was induced. Molecular identification by 18S rDNA sequencing revealed that the isolates were all chlorophytes belonging to four different families.

show abstract

Characterization of the thermal-tolerant mutants of Chlorella sp. with high growth rate and application in outdoor photobioreactor cultivation

Cited by 94 publications

References 11 publications

High-density growth and crude protein productivity of a thermotolerant Chlorella vulgaris: production kinetics and thermodynamics

High-density growth and crude protein productivity of a thermotolerant Chlorella vulgaris: production kinetics and thermodynamics

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Isolation and Characterization of New Temperature Tolerant Microalgal Strains for Biomass Production

Contact Info

Product

Resources

About