Characterization of the Viral RNA Species of Prototype Dengue Viruses

Vezza, Anne C.; Rosen, Léon; Repik, Patricia M.; Dalrymple, Joel M.; Bishop, David H. L.

doi:10.4269/ajtmh.1980.29.643

Cited by 48 publications

(21 citation statements)

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“…Oligonucleotide fingerprints of each of the four dengue virus serotypes are unique (41). Analysis of multiple isolates of dengue 1 and 2 viruses during epidemic conditions, during interepidemic years, and from different geographic areas has indicated that strains of virus from each major geographic region are similar to each other and distinct from viruses isolated from other areas (42)(43).…”

Section: Molecular Epidemiologymentioning

confidence: 99%

“…Dengue virus strain variation has been investigated by comparing the oligonucleotide "fingerprints" produced following RNase Tl digestion of genomic RNA and separation of the labeled oligonucleotides by two-dimension gel electrophoresis (41,43). Oligonucleotide fingerprints of each of the four dengue virus serotypes are unique (41).…”

Section: Molecular Epidemiologymentioning

confidence: 99%

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Current Research on Dengue

Gubler

1987

Advances in Soil Science

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Section: Molecular Epidemiologymentioning

confidence: 99%

Section: Molecular Epidemiologymentioning

confidence: 99%

Current Research on Dengue

Gubler

1987

Advances in Soil Science

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“…Analysis of the genomes of prototype strains of the four serotypes produced unique fingerprints for each, with only 7~ of large T1 oligonucleotides conserved (Vezza et al, 1980). DNA-RNA hybridizations between serotypes revealed sequence homologies of 23 to 73 ~ with most homology between types 1 and 4 and least homology between type 2 and other dengue serotypes (Blok, 1985).…”

Section: Introductionmentioning

confidence: 99%

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Walker

Henchal

Blok

et al. 1988

Journal of General Virology

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SUMMARYDengue type-2 viruses isolated in metropolitan Bangkok during 1980 (Bangkok/80) were characterized by oligonucleotide fingerprinting, restriction enzyme (RE) mapping and antigenic analysis using monoclonal antibody probes. Of 10 isolates analysed by oligonucleotide fingerprinting, nine were very closely related, showing 72-5 ~o to 91.4 ~ oligonucleotide homology. One isolate (D80-141) produced a distinctly different fingerprint (55.7~ to 58"0~o homology) and was less related to other Bangkok/80 dengue-2 virus isolates than to a 1964 Bangkok isolate (16681). RE mapping conducted on complementary dsDNA prepared from three Bangkok/80 isolates, strain 16681 and the prototype New Guinea C strain confirmed that D80-141 was genetically distinct. On antigenic analysis, only one of 22 monoclonal antibody probes produced against representative 1980 Bangkok dengue-2 isolates, D80-100 and D80-141, was able to distinguish between these virus strains. Monoclonal antibody 47-10/10, prepared using D80-100 virus and directed at the NS1 non-structural glycoprotein, had a significantly lower (100-fold) solid phase radioimmune assay endpoint titre for D80-141 antigen than for D80-100 antigen. By the indirect immunofluorescence assay, 47-10/10 had lower antibody endpoint titres against D80-141, the NGC strain and 13 (12~o) of 110 Bangkok/80 isolates than to a control antibody preparation. These results suggest that strain D80-141 represents a second minor topotype of dengue-2 which was circulating concurrently with the major endemic topotype in Bangkok in early 1980.

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“…T1 oligonucleotide fingerprinting has, for many years, been the basic tool of molecular epidemiologists for detecting genetic variation in RNA viruses (Clewley et al, 1977;E1 Said et al, 1979;Robson et al, 1979;Trent & Grant, 1980;Vezza et al, 1980;Nottay et al, 1981 ; et al, Repik et al, 1983 ;Trent et al, 1983). This technique is sensitive and reproducible and provides answers to epidemiological questions.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Epidemiological Studies of Dengue Type 2 Viruses by Hybridization Using Synthetic Deoxyoligonucleotides as Probes

Kerschner¹,

Vorndam²,

Monath³

et al. 1986

Journal of General Virology

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SUMMARYA rapid nucleic acid hybridization procedure was developed for examining the genotypic variation of dengue type 2 viruses (DEN 2) having distinct RNase T1 fingerprints and isolated from different geographical areas. Synthetic DNA hybridization probes were constructed complementary in nucleotide sequence to coaimon and unique RNase TI oligonucleotides of topotype viruses from Puerto Rico/South Pacific, Jamaica, the Seychelles, Thailand/Burma and Africa. Hybridization probes with both type-and topotype-specific reactivities were observed, as were probes specific for two or more of the DEN 2 topotypes. These results confirm geographical movement of topotype virus strains and suggest possible origins. Detection of DEN 2 RNA by hybridization is a rapid and reproducible method that can be modified and applied as a viable alternative to the laborious T1 oligonucleotide fingerprinting.

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Characterization of the Viral RNA Species of Prototype Dengue Viruses

Cited by 48 publications

References 0 publications

Current Research on Dengue

Current Research on Dengue

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Genetic and Epidemiological Studies of Dengue Type 2 Viruses by Hybridization Using Synthetic Deoxyoligonucleotides as Probes

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