Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia

Malik, Amarila; Santoso, Iman; Yehuda, Andi; Freisleben, Seruni K.U.; Wanandi, Septelia Inawati; Huber, Harald; Luthfa, Zessinda; Saleh, Rosari; Freisleben, Hans-Joachim

doi:10.5454/mi.8.1.3

Cited by 3 publications

(5 citation statements)

References 25 publications

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“…2 Sulfolobales and Thermoplasmatales produce high amounts of unique tetraether lipids to build their cell membranes. 3 Whereas Sulfolobales have a cell wall, Thermoplasmatales do not; their lipid membrane is naked and easily accessible.…”

Section: Discussionmentioning

confidence: 99%

Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

et al. 2017

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“…Thermoplasma acidophilum ( T.a. ) DSM 1728 Göttingen, Germany, or isolated from the Indonesian volcano Tangkuban Perahu was cultured micro-aerobically in 1- or 2-L flasks on a shaker [11] or in 5-, 10- or 50-L fermenters according to [12] at 39–59 °C and pH 1–2 in Freundt’s medium containing sulfuric acid adjusted to the desired pH.…”

Section: Growth Of Thermoplasmamentioning

confidence: 99%

“…Thermoplasma acidophilum DSM 1728 grows up to OD (λ = 578 nm) of 0.6, at 39 °C within 200–210 h, at 49 °C within 70 h, at 59 °C within 42 h [12], wild type from Tangkuban Perahu, Indonesia, identified as mixtures of T. acidophilum and volcanium [11], grows up to OD of 0.35 within 160 h in culture flasks on a shaker without automated regulation of growth conditions at 55–56 °C in 1–5 sequential culture generations and up to OD 0.4 within 240 h [11]. In an optimized computer-regulated fermenter culture wild type grew up rapidly to an OD of almost 1.0 within 2-3 days (Figure 1); this culture has not yet been further investigated.…”

Section: Growth Of Thermoplasmamentioning

confidence: 99%

“…Culture growth of Thermoplasma . Growth of Thermoplasma acidophilum DSM 1728, Göttingen, Germany, at 39 °C, 49 °C, and 59 °C and of wild type Thermoplasma harvested from a Tangkuban Perahu crater, grown at 55–56 °C in 1-Liter culture flasks without automated control (TP 55); growth of the latter is similar to that of the former at 39 °C to a maximum OD of 0.35–0.4 and at 59 °C in a computer-regulated 2-L fermenter (TP 59); OD, optical density at λ = 578 nm; in all cultures, pH was below 2 (at the beginning of the cultures adjusted to 1.5 and re-adjusted, if necessary); values adapted from [11,12]. …”

Section: Figurementioning

confidence: 99%

“…This research was transferred to Indonesia [6,7,8], since Thermoplasma and other Archaeal species had been found in Indonesian volcanoes [9]. Meanwhile, Archaea have been isolated and cultured from there [10,11] and research will be continued from wild type species, until Indonesian laboratory stems will have been established.…”

Section: Introductionmentioning

confidence: 99%

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The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

Freisleben

2019

IJMS

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The main phospholipid (MPL) of Thermoplasma acidophilum DSM 1728 was isolated, purified and physico-chemically characterized by differential scanning calorimetry (DSC)/differential thermal analysis (DTA) for its thermotropic behavior, alone and in mixtures with other lipids, cholesterol, hydrophobic peptides and pore-forming ionophores. Model membranes from MPL were investigated; black lipid membrane, Langmuir-Blodgett monolayer, and liposomes. Laboratory results were compared to computer simulation. MPL forms stable and resistant liposomes with highly proton-impermeable membrane and mixes at certain degree with common bilayer-forming lipids. Monomeric bacteriorhodopsin and ATP synthase from Micrococcus luteus were co-reconstituted and light-driven ATP synthesis measured. This review reports about almost four decades of research on Thermoplasma membrane and its MPL as well as transfer of this research to Thermoplasma species recently isolated from Indonesian volcanoes.

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How Can Immuno suppression After Organ Transplantation be Made More Effective and Safer? – A Review on Liposomal Formulations with Consideration of Archaeal Tetraetherlipid

Purwaningsih

Oertl

Freisleben

et al. 2021

Biomed. Pharmacol. J.

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Immune-suppressive agents such as methylprednisolone and cyclosporine exert tremendous side effects, because of high dosage and long-term application required for immune suppression after organ transplantation. Major side effects of methylprednisolone include bleeding of the gastro-intestinal tract, hypertension, and osteoporosis, whereas cyclosporine is nephrotoxic. Liposomes are phospholipid particles that allow delivery of drugs preferentially to the reticuloendothelial system. They can be prepared from phospholipids such as lecithin from soybean or egg yolk, other specific or modified lipids or from membrane-spanning tetraether lipid (TEL), which can be extracted and purified from archaeal cell membranes. One advantage in the use of liposomal application is reduced toxicity of many drugs. We report on various liposomal preparations of cyclosporine, methylprednisolone (L-MPL) and its palmitate derivative (L-MPLP). It has been documented that liposomal cyclosporine A (L-CsA), 1.75 mg/kg/day for seven days has potential for use as an immune-suppressive agent in rats with increased efficacy and decreased nephrotoxicity compared to commercially available forms of intravenous CsA. Liposomal methylprednisolone (L-MPL) 2 mg/kg, intravenously (IV), twice a week shows significantly prolonged cardiac allograft survival in rats and tissue-selective sequestration of the drug in comparison with the same dosage regimen of methylprednisolone in solution, administered daily. We report on organ distribution of L-MPLP in rats after intraperitoneal (IP) administration. Conclusion: Liposomal preparations of immunosuppressants have significantly higher immune-suppressive potential and lower toxicity than non-liposomal preparations. Bipolar TEL can be extracted, fractionated and purified from archaea to form stable liposomes which are extremely resistant, even to gastric fluid. Hence, TEL liposomes allow (besides IV and IP) for oral administration of immuno suppressants after organ transplantation with pharmacological and toxicological advantages as common liposomal phospholipid bilayer preparations.

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Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia

Cited by 3 publications

References 25 publications

Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

How Can Immuno suppression After Organ Transplantation be Made More Effective and Safer? – A Review on Liposomal Formulations with Consideration of Archaeal Tetraetherlipid

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