Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan

Kawahara, Ryuji; Seto, Kazuko; Taguchi, Masumi; Nakajima, Chie; Kumeda, Yuko; Suzuki, Yasuhiko

doi:10.1128/jcm.01263-15

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…E. coli O157:H7 with identical PFGE pattern (100% similarity) carry different virulence genes and different drug resistance phenotypes, suggesting that the virulence and drug resistance carried by E. coli O157:H7 may be acquired or lost during the evolution and transfer of the same cluster of strains. The β-lactam-resistant E. coli O157:H7 may give β-lactam resistance to other pathogenic enterobacteria via plasmidmediated conjugation, thereby posing potential challenges in the management of their associated infectious disease in cattle (60). E. coli O157:H7 was prevalent in 2-15% population of cattle and other animals in China (47).…”

Section: Discussionmentioning

confidence: 99%

First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

Tong

Zhang

et al. 2021

Front. Vet. Sci.

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The bovine Escherichia coli O157:H7 is a major foodborne pathogen causing severe bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Cattle are recognized major reservoir and source of E. coli O157:H7. We investigated the antibiotic resistance, molecular profiles, and intrinsic relationship between 21 isolates of E. coli O157:H7 from cattle farms and slaughtering houses in Xinjiang. Using pulsed-field gel electrophoresis (PFGE) molecular typing, two types of PFGE were revealed through cluster analysis, including clusters I and II, with 66 and 100% similarity of PFGE spectra between 21 isolates. We also detected that 18 isolates (86%) carried at least one virulence gene, 16 isolates (76%) carried the eae gene, and 7 (33%) carried the stx1 + stx2 + eae + hly + tccp genes. Eighteen isolates were susceptible to antibiotics. Three isolates were resistant to antibiotics, and two were multidrug resistant. One of the two multidrug-resistant isolates detectably carried the blaCTX−M−121 gene. This is the first finding of the blaCTX−M−121 gene detected in E. coli O157:H7 isolated from cattle in Xinjiang. The blaCTX−M−121 gene is transferable between the bacterial strains via plasmid transmission. The results indicated that E. coli O157:H7 may have undergone clonal propagation in cattle population and cross-regional transmission in Xinjiang, China.

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Section: Discussionmentioning

confidence: 99%

First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

Tong

Zhang

et al. 2021

Front. Vet. Sci.

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“…The presence of pAmpC genes responsible for the AmpC phenotype was detected by multiplex PCR, as described by [ 8 ]. bla TEM genes were identified by sequencing, as previously described [ 9 ], and bla SHV genes were also identified by sequencing with primers designed in this study (5′-CACTCAAGGATGTATTGTGGTTATGC-3′ and 5′-GCTACGAGCCGGATAACGC-3′).…”

Section: Methodsmentioning

confidence: 99%

Dissemination of Extended-Spectrumβ-Lactamase- and AmpCβ-Lactamase-ProducingEscherichia coliwithin the Food Distribution System of Ho Chi Minh City, Vietnam

Nguyen

et al. 2016

BioMed Research International

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To investigate the dissemination of ESBL/pAmpC-producing E. coli within the food distribution system of Ho Chi Minh City (HCMC), Vietnam, the prevalence of ESBL/pAmpC-producing E. coli strains in chicken meat, pork, beef, and fish/shrimp samples obtained from slaughterhouses, a wholesale market, and supermarkets was examined. Among the total of 330 collected food samples, ESBL/pAmpC-producing E. coli was detected in 150 samples (45.5%). The highest prevalence of these isolates was in chicken meat (76/82, 92.7%), followed by pork (32/92, 34.8%), beef (18/74, 34.3%), and fish/shrimp (24/82, 29.3%). A total of 342 strains of ESBL/pAmpC-producing E. coli were isolated from 150 positive food samples. The most prevalent genes responsible for ESBL or pAmpC activity belonged to the CTX-M-9 (110/342, 31.2%), CTX-M-1 (102/342, 29.8%), and CIT (118/342, 34.5%) groups. To our knowledge, this is the first report of the high occurrence of pAmpC (37.1%) in animal-based food in Vietnam. Among the 342 total ESBL/pAmpC-producing E. coli isolates, 276 (80.7%) were resistant to at least 6 antibiotic agents. Notably, high percentages of resistance to ciprofloxacin and fosfomycin were found in isolates from chicken (80.5% and 50.8%, resp.). These findings demonstrate that animal-based food products in HCMC represent a major reservoir of ESBL/pAmpC-producing E. coli.

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“…Recent studies reported several β‐lactamase genes, such as bla CTX‐M , bla AmpC , bla CMY‐1 , bla TEM and bla DHA‐1 in STEC serotypes O26:H11, O64, O91:H14, O106:H18, O111:H8 and O157, recovered from humans and animals; some of these β‐lactamase genes were encoded on plasmids (Arvand et al, ; Dutta et al, ; Ishii et al, ; Iweriebor et al, ; Jost et al, ; Valat et al, ). Seven O157 isolates harbouring bla CMY‐2 , obtained from clinical samples at the Osaka Prefecture, Japan, had antibiotic resistance genes encoded on a IncI1 plasmid that has high transmissibility across bacterial species (Kawahara et al, ). In Ireland, multidrug‐resistant (MDR) non‐O157 STEC isolated from cattle farms and abattoirs were found to carry Class 1 integrons with antibiotic resistance genes embedded on plasmids (Kennedy et al, ).…”

Section: Acquisition and Transmission Of Antibiotic Resistance In Ar‐mentioning

confidence: 99%

“…Seven O157 isolates harbouring bla CMY-2 , obtained from clinical samples at the Osaka Prefecture, Japan, had antibiotic resistance genes encoded on a IncI1 plasmid that has high transmissibility across bacterial species (Kawahara et al, 2015). In Ireland, multidrug-resistant were found to carry Class 1 integrons with antibiotic resistance genes embedded on plasmids (Kennedy et al, 2017).…”

Section: Acqu Is Iti On and Tr An S Miss I On Of Antib I Oti C Re Smentioning

confidence: 99%

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

Mir

Kudva

2018

Zoonoses and Public Health

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Shiga toxin‐producing Escherichia coli (STEC) are foodborne pathogens that can cause severe diseases, including bloody diarrhoea and kidney failure, in humans, while remaining harmless to its primary reservoir hosts, cattle. Antibiotics such as azithromycin, fosfomycin and meropenem are being used and recommended in the treatment of early‐stage STEC (mainly E. coli O157:H7) infections, as these are reportedly effective in preventing Shiga toxin release and kidney failure while eliminating the pathogen. However, antibiotic resistance among STEC isolates could negatively impact these and other similar treatment options while contributing towards the spread of antibiotic resistance genes especially if encoded on mobile genetic elements like plasmids. Antibiotic resistance among STEC isolates recovered from animals and patients is being reported globally. A comprehensive understanding of the prevalence of antibiotic‐resistant STEC (AR‐STEC) and the mechanisms promoting this resistance among these bacteria could help direct therapies and develop strategies to effectively reduce/eliminate these pathogens. Here, we have reviewed literature from the past three decades to gain insights on this prevalence and its impact on human infections. In addition, we have reviewed various strategies proposed by researchers to control STEC that in turn would be applicable to AR‐STEC as well.

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Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan

Cited by 9 publications

References 17 publications

First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

Dissemination of Extended-Spectrumβ-Lactamase- and AmpCβ-Lactamase-ProducingEscherichia coliwithin the Food Distribution System of Ho Chi Minh City, Vietnam

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

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