Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure

Kobayashi, N.; Noel, Eric A.; Barnes, Austin; Watson, Andrea K; Rosenberg, Julian N.; Erickson, Galen E.; Oyler, George A.

doi:10.1016/j.biortech.2013.10.032

Cited by 119 publications

(41 citation statements)

References 28 publications

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“…Statistical analysis has shown that biomass and lipid production of this alga in modified Bold's Basal Medium is significantly higher than that in the normal Bold's Basal Medium. Increase in biomass yield in accordance with change in medium compositions is already reported in another species of Chlorella [27]. However, in the current experiment using modified BBM, increase in yield of both biomass and lipid content observed for Chlorella lobophora in the modified medium.…”

Section: Biomass and Lipid Productivity Of Algasupporting

confidence: 84%

Biomass Productivity and Fatty Acid Composition of Chlorella lobophora V M Andreyeva, a Potential Feed Stock for Biodiesel Production

Kookal¹,

Santhakumaran²,

Ray³

2015

AJPS

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Green algae represent the fastest producible source of biomass that has diverse uses as biofuel, food, feed-stocks and the like. The biomass is rich in oils, which can be used as biofuel and the cultivation may be used to purify water as well. Productivity and quality of algal biomass depends on species and strains as well as environmental conditions of growth. Therefore, identification of new species and strains as well as standardization of media composition and environmental conditions for optimum yield of specific products, especially oil has a high relevance in algal technological research. In this connection, biomass productivity and oil yield of a local strain of Chlorella lobophora in BBM and modified BBM media are compared. Chemical characterization of the oil and feasibility of biodiesel production from the extracted oil is also assessed. Biomass productivity and the lipid productivity of the alga in both the media are found to be significantly different (P < 0.05). Fatty acid profiling of the oil extracted from the dried algal biomass using GC-MS analysis reveals that Palmitic acid (16:0) and Oleic acid (18:1) are the major fatty acids and nutraceutically important fatty acids are also present. The oil can be effectively transesterified to methyl esters. FTIR spectroscopy confirms the quality of the biodiesel produced. As the potentials of this local algal strain for biomass production and oil yield is confirmed, further standardization of environment conditions for better oil yield and quality biomass production is progressing to optimize its value as globally competent nutraceutical and biofuel resource.

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Section: Biomass and Lipid Productivity Of Algasupporting

confidence: 84%

Biomass Productivity and Fatty Acid Composition of Chlorella lobophora V M Andreyeva, a Potential Feed Stock for Biodiesel Production

Kookal¹,

Santhakumaran²,

Ray³

2015

AJPS

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“…This fact is due to the biological transformation of the manure organic matter into biogas which took place during the anaerobic digestion (AD) of sample Pre (Tambone et al, 2009). Furthermore, a higher amount of ash was observed for sample Dig R due to its lower volatile matter content (Kobayashi et al, 2013). This trend was also corroborated by ICP analysis; the content of individual inorganic components into the ash was higher for sample Dig R.…”

Section: Physico-chemical Characterization Of Manure Samplessupporting

confidence: 76%

Life cycle assessment of swine and dairy manure: Pyrolysis and combustion processes

Fernández-López

Puig-Gamero

López-González

et al. 2015

Bioresource Technology

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“…, chlorophylls, carotenoids, phenols, tocopherols, sterols). This may indicate that the cellular metabolism of UTEX 265 remains more active than lipid storage during heterotrophy, as observed previously with C. sorokiniana strains UTEX 2714 and CS-01 as well as Chlamydomonas reinhardtii [56], [103]. In a complementary case, C. protothecoides UTEX 411 produced approximately 10% membrane lipids during heterotrophy [+] ( e.g.…”

Section: Discussionmentioning

confidence: 53%

Comparative Analyses of Three Chlorella Species in Response to Light and Sugar Reveal Distinctive Lipid Accumulation Patterns in the Microalga C. sorokiniana

et al. 2014

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While photosynthetic microalgae, such as Chlorella, serve as feedstocks for nutritional oils and biofuels, heterotrophic cultivation can augment growth rates, support high cell densities, and increase triacylglycerol (TAG) lipid content. However, these species differ significantly in their photoautotrophic and heterotrophic characteristics. In this study, the phylogeny of thirty Chlorella strains was determined in order to inform bioprospecting efforts and detailed physiological assessment of three species. The growth kinetics and lipid biochemistry of C. protothecoides UTEX 411, C. vulgaris UTEX 265, and C. sorokiniana UTEX 1230 were quantified during photoautotrophy in Bold's basal medium (BBM) and heterotrophy in BBM supplemented with glucose (10 g L−1). Heterotrophic growth rates of UTEX 411, 265, and 1230 were found to be 1.5-, 3.7-, and 5-fold higher than their respective autotrophic rates. With a rapid nine-hour heterotrophic doubling time, Chlorella sorokiniana UTEX 1230 maximally accumulated 39% total lipids by dry weight during heterotrophy compared to 18% autotrophically. Furthermore, the discrete fatty acid composition of each strain was examined in order to elucidate lipid accumulation patterns under the two trophic conditions. In both modes of growth, UTEX 411 and 265 produced 18∶1 as the principal fatty acid while UTEX 1230 exhibited a 2.5-fold enrichment in 18∶2 relative to 18∶1. Although the total lipid content was highest in UTEX 411 during heterotrophy, UTEX 1230 demonstrated a two-fold increase in its heterotrophic TAG fraction at a rate of 28.9 mg L−1 d−1 to reach 22% of the biomass, corresponding to as much as 90% of its total lipids. Interestingly, UTEX 1230 growth was restricted during mixotrophy and its TAG production rate was suppressed to 18.2 mg L−1 d−1. This constraint on carbon flow raises intriguing questions about the impact of sugar and light on the metabolic regulation of microalgal lipid biosynthesis.

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Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure

Cited by 119 publications

References 28 publications

Biomass Productivity and Fatty Acid Composition of Chlorella lobophora V M Andreyeva, a Potential Feed Stock for Biodiesel Production

Biomass Productivity and Fatty Acid Composition of Chlorella lobophora V M Andreyeva, a Potential Feed Stock for Biodiesel Production

Life cycle assessment of swine and dairy manure: Pyrolysis and combustion processes

Comparative Analyses of Three Chlorella Species in Response to Light and Sugar Reveal Distinctive Lipid Accumulation Patterns in the Microalga C. sorokiniana

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