Characterization of three GLOBOSA -like MADS-box genes from maize: evidence for ancient paralogy in one class of floral homeotic B-function genes of grasses

Münster, Thomas; Wingen, Luzie U.; Faigl, Wolfram; Werth, S.; Saedler, Heinz; Theißen, Günter

doi:10.1016/s0378-1119(00)00556-4

Cited by 103 publications

(88 citation statements)

References 35 publications

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“…This is in keeping with the results of earlier in situ hybridization experiments that showed no STS1 expression in the developing gynoecium of tassel florets (Whipple et al, 2004). However, Münster et al (2001) report expression of sts1, as well as Zmm18/29, in the gynoecia of female florets, as assessed by RNA in situ hybridization. In addition, in maize expression browsers (Sekhon et al, 2011;qTELLER.com), sts1 expression is found in silks.…”

Section: Sts1 Protein Localization During Ear and Tassel Floret Develsupporting

confidence: 91%

“…ZMM18 and ZMM29 protein products are 97.2% similar to each other, and they are also very similar to STS1 (;84% similar) (Münster et al, 2001). In addition, sts1, Zmm18, and Zmm29 have very similar expression patterns (Münster et al, 2001;Whipple et al, 2004). Given these similarities, it is surprising that mutation of sts1 alone is sufficient to produce a strong loss of B function phenotype and suggests that Zmm18 and Zmm29 are not fully functionally redundant with Zmm16/sts1.…”

Section: Rnai Knockdown Of Sts1 Paralogs Has No Effect On Floret Phenmentioning

confidence: 83%

“…Zmm16/sts1 belongs to the PI-2 clade, while maize has two representatives in the PI-1 clade (Zmm18 and Zmm29) that resulted from a recent tandem duplication (Münster et al, 2001). ZMM18 and ZMM29 protein products are 97.2% similar to each other, and they are also very similar to STS1 (;84% similar) (Münster et al, 2001). In addition, sts1, Zmm18, and Zmm29 have very similar expression patterns (Münster et al, 2001;Whipple et al, 2004).…”

Section: Rnai Knockdown Of Sts1 Paralogs Has No Effect On Floret Phenmentioning

confidence: 99%

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The Maize PI/GLO Ortholog Zmm16/sterile tassel silky ear1 Interacts with the Zygomorphy and Sex Determination Pathways in Flower Development

et al. 2015

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In monocots and eudicots, B class function specifies second and third whorl floral organ identity as described in the classic ABCE model. Grass B class APETALA3/DEFICIENS orthologs have been functionally characterized; here, we describe the positional cloning and characterization of a maize (Zea mays) PISTILLATA/GLOBOSA ortholog Zea mays mads16 (Zmm16)/ sterile tassel silky ear1 (sts1). We show that, similar to many eudicots, all the maize B class proteins bind DNA as obligate heterodimers and positively regulate their own expression. However, sts1 mutants have novel phenotypes that provide insight into two derived aspects of maize flower development: carpel abortion and floral asymmetry. Specifically, we show that carpel abortion acts downstream of organ identity and requires the growth-promoting factor grassy tillers1 and that the maize B class genes are expressed asymmetrically, likely in response to zygomorphy of grass floral primordia. Further investigation reveals that floral phyllotactic patterning is also zygomorphic, suggesting significant mechanistic differences with the wellcharacterized models of floral polarity. These unexpected results show that despite extensive study of B class gene functions in diverse flowering plants, novel insights can be gained from careful investigation of homeotic mutants outside the core eudicot model species.

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Section: Sts1 Protein Localization During Ear and Tassel Floret Develsupporting

confidence: 91%

Section: Rnai Knockdown Of Sts1 Paralogs Has No Effect On Floret Phenmentioning

confidence: 83%

Section: Rnai Knockdown Of Sts1 Paralogs Has No Effect On Floret Phenmentioning

confidence: 99%

See 1 more Smart Citation

The Maize PI/GLO Ortholog Zmm16/sterile tassel silky ear1 Interacts with the Zygomorphy and Sex Determination Pathways in Flower Development

et al. 2015

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“…DNA and RNA Blot Analysis. Maize genomic DNA was extracted from young leaf material using a diethyldithiocarbamate sodium-based protocol (35). DNA blot analysis was performed following described methods (36).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was isolated, separated by electrophoresis, and transferred to positively charged nylon membranes (Pall) as described (35). The following tissues were used for the RNA preparation: leaf blades of 6-wk-old plants, husk leaves covering 0.3-to 2.5-cm immature ears, tassels of 0.5-2.5 cm, and immature ears 0.3-2.5 cm in length.…”

Section: Methodsmentioning

confidence: 99%

Molecular genetic basis of pod corn ( Tunicate maize)

Wingen

Münster

Faigl

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Pod corn is a classic morphological mutant of maize in which the mature kernels of the cob are covered by glumes, in contrast to generally grown maize varieties in which kernels are naked. Pod corn, known since pre-Columbian times, is the result of a dominant gain-of-function mutation at the Tunicate ( Tu ) locus. Some classic articles of 20th century maize genetics reported that the mutant Tu locus is complex, but molecular details remained elusive. Here, we show that pod corn is caused by a cis -regulatory mutation and duplication of the ZMM19 MADS-box gene. Although the WT locus contains a single-copy gene that is expressed in vegetative organs only, mutation and duplication of ZMM19 in Tu lead to ectopic expression of the gene in the inflorescences, thus conferring vegetative traits to reproductive organs.

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Floral Patterning and Control of Floral Organ Formation

Kramer¹

Flowering and Its Manipulation

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Characterization of three GLOBOSA -like MADS-box genes from maize: evidence for ancient paralogy in one class of floral homeotic B-function genes of grasses

Cited by 103 publications

References 35 publications

The Maize PI/GLO Ortholog Zmm16/sterile tassel silky ear1 Interacts with the Zygomorphy and Sex Determination Pathways in Flower Development

The Maize PI/GLO Ortholog Zmm16/sterile tassel silky ear1 Interacts with the Zygomorphy and Sex Determination Pathways in Flower Development

Molecular genetic basis of pod corn ( Tunicate maize)

Floral Patterning and Control of Floral Organ Formation

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