Characterization of Three mnp Genes of Fomitiporia mediterranea and Report of Additional Class II Peroxidases in the Order Hymenochaetales

Abstract

We report the sequence-based characterization and expression patterns of three manganese peroxidase genes from the white rot fungus and grape vine pathogen Fomitiporia mediterranea (Agaricomycotina, Hymenochaetales), termed Fmmnp1, Fmmnp2, and Fmmnp3. The predicted open reading frames (ORFs) are 1,516-, 1,351-, and 1,345-bp long and are interrupted by seven, four, and four introns, respectively. The deduced amino acid sequences encode manganese peroxidases (EC 1.11.1.13) containing 371, 369, and 371 residues, respectively, and are similar to the manganese peroxidases of the model white rot organism Phanerochaete chrysosporium. The expression of the genes is most likely differentially regulated, as revealed by real-time PCR analysis. Phylogenetic analysis reveals that other members of the order Hymenochaetales harbor mnp genes encoding proteins that are related only distantly to those of F. mediterranea. Furthermore, multiple partial lipand mnp-like sequences obtained for Pycnoporus cinnabarinus (Agaricomycotina, Polyporales) suggest that lignin degradation by white rot taxa relies heavily on ligninolytic peroxidases and is not efficiently achieved by laccases only.

Patterns of lignin degradation and oxidative enzyme secretion by different wood- and litter-colonizing basidiomycetes and ascomycetes grown on beech-wood

“…The fungus had been supposed to decompose wood exclusively by the action of high‐redox potential laccases in cooperation with the fungal metabolite hydroxyanthranilic acid acting as low‐molecular mass redox mediator (Eggert et al , 1996, 1997; Herpoël et al , 2000). Our finding of MnOP activities in wood extracts of P. cinnabarinus and the identification of MnP genes in the fungus (Dölz et al , 2007; Morgenstern et al , 2010; Uzan et al , 2010) strongly suggest that it belongs to the abundant group of MnP‐laccase‐producing white‐rot fungi (Hatakka, 1994, 2001; Eggert et al , 1997; Hatakka & Hammel, 2010).…”

Lignin-degrading Peroxidases from Genome of Selective Ligninolytic Fungus Ceriporiopsis subvermispora

“…Interestingly, all the class II peroxidase genes in the C. subvermispora genome appear to be functional, because transcripts for all have been detected in fungal cultures (transcriptomic data recently available under GEO accession GSE34636). This class II expansion is particularly true for the MnP models, which include a total of 13 genes, a greater number than in any sequenced basidiomycete genome except that of Fomitiporia mediterranea (58).…”

8 Degradation and Modification of Plant Biomass by Fungi

“…The only ECM sequence that is placed within one of the lignin-modifying peroxidase groups with strong support is the partial sequence from Gomphus clavatus (Morgenstern et al 2010). …”