Characterization of Three Varieties of <i>Lathyrus sativus</i> L. by Fluorescent Karyotype and RAPD Analysis

Abstract

Summary Three varieties of Lathyrus sativus L. released from Bangladesh Agricultural Research Institute (BARI), viz., BARI Khesari-1, BARI Khesari-2 and BARI Khesari-3, were studied cytogenetically and at the molecular level by using RAPD for genomic characterization. The three varieties were found to posses 2n=14 chromosomes. The karyotype formulae of BARI Khesari-1 and BARI Khesari-2 are 8m + 6sm, while it is 10 m + 4sm in BARI Khesari-3. After orcein and CMA staining, one to two very small chromosome-like bodies were found in some cells of BARI Khesari-1, whereas no such body was observed after DAPI staining. Due to their unique features these can be considered as GC-rich heterochromatic B-chromosomes. In BARI Khesari-2, two pairs of satellites were found, while only one pair was present in BARI Khesari-3. The inverted position of satellited regions indicated the probable occurrence of homozygous inversion. An indication of paracentric inversion regarding DAPI-banding pattern was found in pair V of BARI Khesari-2 and thus could be considered as marker chromosomes for this variety. The three varieties have distinct CMA-and DA-PI-banding patterns. In BARI Khesari-2, a total of 12 C-bands were found on 10 chromosomes (out of 14 chromosomes). On the basis of band position and length, a deep correlation between C-banding and DAPI-banding pattern was found suggesting the heterochromatic nature of DAPI bands. Each variety showed different RAPD fingerprinting with 59.09% polymorphisim. In addition, a number of variety-specific unique RAPD bands was observed. Therefore, each variety could be characterized on the basis of karyotype and RAPD analysis.

Genetic diversity and population structure analysis of Grass pea (Lathyrus sativus L.) accessions collected from North-Western Ethiopia using SSR markers

“…Crop characterization based on morphology is generally not reliable due to phenotypic plasticity. Hence, for signi cant progress in the breeding program of grass pea, the use of molecular marker and genomic knowledge is a pre-requisite (Akter et al, 2015). Molecular markers that have so far been used successfully for genomic studies in L.sativus includes random ampli cation of polymorphic DNA (RAPD) (Barik et al, 2007;Croft et al, 1999), restriction fragment length polymorphism (RFLP) (Chtourou-Ghorbel et al, 2001), ampli ed fragment length polymorphism (AFLP) (Tavoletti and Iommarini, 2007), intersimple sequence repeat (ISSR) (Belaid et al, 2006), and simple sequence repeats (SSR) (Wang et al, 2015;Shiferaw et al, 2012;Lioi et al, 2011).…”

The Electrophoretic Contribution to the Solutions of Taxonomical Problems of Some Lathyrus L. Taxa Growing in Turkey

“…Different techniques including isozyme analysis (Sammour et al, 2019), fatty acid analysis (Bagci et al, 2001;Arslan, 2017), electrophoresis (El-Shanshoury, 1997;Przybylska et al, 2000;Ben Brahim et al, 2002;Radwan et al, 2013), RFLP, AFLP (Badr et al, 2002;Belaid et al, 2006) and RAPD techniques (Chtourou-Ghorbel et al, 2001Akter et al, 2015) are used to classify the Lathyrus taxa as well as morphological characters. Seed storage proteins are significant biochemical markers because they are less affected by the environment and they are used in genetic variation studies and to characterize cultivars (Sofailan et al, 2015).…”

Comparative Karyotype and RAPD Analysis of Four <i>Oxalis</i> L. Species

“…It helps to provide information regarding the reshuffling of GC-and AT-rich repeats in the genome. In addition, chromosomal abnormality could also be detected by this method (Schweizer 1976, Alam and Kondo 1995, Kondo and Hizume 1982, Jessy et al 2005, Akter et al 2015, Hossain et al 2016.…”