In the present study, fatty acid composition in mature seeds of the Vicia L. taxa were determined by using gas chromatography. Present results indicate that the seed oils of studied Lathyrus taxa have palmitic acid (C16:0) and stearic acid (C18:0) as major saturated fatty acids. Moreover, the present data showe that oleic acid (C18:1), linoleic acid (C18:2 n 6) and α-linolenic acid (C18:3 n 3) were major unsaturated fatty acids in studied taxa. The highest oleic acid (C18:1 n 9) content was found in V. crocea (Desf.) B. Fedstch. (26.5%) but it was found at the lowest level in V. mollis (7.8%). In addition, present study determine that investigated Vicia species have linoleic acid (46.4-79.1%) and linolenic acid (3.8-21.3%) contents.
Abstract.-In the present study, levels of vitamin and fatty acid composition in mature seeds of some Lathyrus L. taxa [L. roseus Stev., L. tuberosus L.,var. affinis (Guss.) Arcang.] were determined by using HPLC (high-performance liquid chromatography) and VPC (vapour gas chromatography). Palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1 n-9), linoleic acid (C18:2 n-6) and α-linolenic acid (C18:3 n-3) were found as major fatty acid components in all studied eleven taxa. In addition, δ-tocopherol, α-tocopherol, α-tocopherol acetate and vitamin D3 were found highest level and retinol, retinol acetate, vitamin D2 and vitamin C content (apart from L. belinensis and L. hirsutus) were found lowest ratio in the examined Lathyrus taxa.
Key words : Lathyrus -fatty acids -vitamins -HPLC -VPC.
Résumé.-Dans la présente étude, les niveaux en vitamines et en acides gras des graines adultes de Lathyrusvar. affinis (Guss.) Arcang.] ont été déterminés par chromatographie (CLHP et CG). Les acides palmitique (C16:0), stéarique (C18:0), oléique (C18:1 n-9), linoléique (C18:2 n-6) et α-linolénique (C18:3 n-3) ont été observés comme constituants des onze taxons étudiés. En plus, les δ-tocopherol, α-tocophérol, acétate de l'α-tocophérol et la vitamine D3 ont été trouvés en plus grande quantité, les rétinol, acétate de rétinol, vitamine D2 et vitamine C le l'ont été en quantité faible (sauf L. belinensis et L. hirsutus) pour les Lathyrus examinés.
RESUMENCompuestos biológicos, capacidad atrapadora de radicales libres y actividad antimicrobiana de las semillas de Nepeta italica L. y Sideritis montana L. subsp. montana de Turquía.Este estudio ha determinado algunos compuestos bioló-gicos (ácidos grasos, vitaminas liposolubles, esteroles y flavonoides), capacidad atrapadora de radicales libres, y actividades antimicrobianas de las semillas de Nepeta italica L. y Sideritis montana L. subsp. montana. Se encontró que el áci-do palmítico (C16:0; 8.54±0.13-3.05±0.04%), ácido oleico (C18:1 n9, 22.41±0.8-18.83±0.1%) y α-linolénico (C18:3 n 3;39.56±0.67-77.04±2.07%) eran mayoritarios en ambas semillas de Nepeta italica L. y Sideritis montana L. subsp. montana. Además, se determinó que tanto Nepeta italica L. como Sideritis montana L. subsp. montana contenían estigmasterol (630.07±1.81μg/g; 80,74±0.71μg/g, respectivamente) y ergosterol (11.01±0.14 μg/g; 161.32±0.63 μg/g respectivamente) y esta última además beta-sitosterol (2.93±0.03μg/g). Además, los resultados muestran que Nepeta italica L. contiene morin (37.79±1.09μg/g), catequina (124.39±2.23μg/g) y naringina (475.96±3.57μg/g) y Sideritis montana L. subsp. montana contiene morin (188.41±2.53μg/g), catequina (64.14±1.86μg/g), naringenina (38.34±1.78 μg/g), como principales flavonoides. También, se determinó que los extractos metanólicos de Nepeta itálica L. y Sideritis montana L. subsp. montana fueron eficaces contra los radicales DPPH. Los resultados obtenidos en el presente estudio mostraron que las vitaminas, los flavonoides y los ácidos grasos de los extractos en las semillas de Nepeta itálica L. y Sideritis montana L. subsp. montana impidieron el crecimiento de los microorganismos utilizados en el ensayo, en diferentes proporciones.
PALABRAS CLAVE: Ácidos grasos -Actividad antimicrobiana -DPPH radical scavenging actividad -Esteroles
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