Characterization of tilapia (Oreochromis niloticus) skin gelatin extracted with alkaline and different acid pretreatments

Niu, Lihong; Zhou, Xin; Yuan, Chuqiao; Bai, Yun; Lai, Keqiang; Yang, Fuxin; Huang, Yiqun

doi:10.1016/j.foodhyd.2013.04.014

Cited by 76 publications

(61 citation statements)

References 31 publications

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“…Some readers may get the mistaken impression that the research article describes the collagen extraction at a higher temperature. Authors used the hot water extraction method that was commonly used for the extraction of the gelatin (a hydrolyzed form of collagen) (Niu et al 2013). 4.…”

Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 99%

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Technical note on the isolation and characterization of collagen from fish waste material

Kumar

Rani

2017

J Food Sci Technol

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The aim of this manuscript was to evaluate the major technical problems on the isolation and characterization of the collagen from fish waste materials that were usually faced by the growing researchers. Although the original research article published by authors contributed new information to the literature, some of them were failed to provide sufficient details in order to reproduce the study as well as could not adequately interpret/compared the results with other publications. Therefore, it is required to research the technical problems during the isolation and characterization of the collagen. This technical note provides the information which is crucial for the reader's and growing researchers for understanding as an essential part of the published research studies about the collagen extraction and characterization. Hence, this technical note may be helpful to those working on the collagen extraction and characterization from fish/marine waste materials.

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Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 99%

“…Authors described that minced skins were continuously stirred overnight in distilled water at high temperature. Gelatin was mainly extracted in the water due to its higher solubility (Niu et al 2013). However, fish skin collagen was extracted in the acidic conditions.…”

Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 99%

Technical note on the isolation and characterization of collagen from fish waste material

Kumar

Rani

2017

J Food Sci Technol

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“…Trilaksani et al (2006) telah meneliti pemanfaatan gelembung renang ikan patin Pangasius hypopthalmus sebagai bahan baku pembuatan Isinglass dengan kandungan protein 94,38 -94,63%. Isinglass umumnya masih digunakan sebagai fining agent pada industri bir di Eropa (Niu et al 2013). Kandungan protein yang tinggi diduga berasal dari kandungan protein jaringan ikat yang tidak larut dalam air.…”

Section: Pendahuluanunclassified

Characterization of Collagen from Swim Bladder Waste of Yellow-pike (Muraenesox talabon) by Acid and Hydrothermal Extraction

2017

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AbstrakProduksi perikanan tangkap Indonesia mencapai ±6,4 juta ton pada tahun 2014. Jumlah tersebut diperkirakan akan menghasilkan limbah perikanan yang besar, salah satunya gelembung renang yang berpotensi mengandung kolagen, sehingga perlu dikembangkan. Penelitian ini bertujuan menentukan karakteristik gelembung renang (proporsi dan kandungan kimia) dan karakteristik kolagen (rendemen dan sifat fisikokimia) yang diekstrak dari gelembung renang ikan cunang (Muraenesox talabon). Limbah gelembung renang memiliki proporsi 0,57-0,67% dari bobot tubuh ikan cunang dan 24,74% protein/100 gram basah (setara 93,39% protein/100 gram kering). Limbah tersebut memiliki potensi kolagen yang dicirikan dengan proporsi asam amino glisin, prolin, hidroksiprolin dan alain yang tinggi. Proses ekstraksi asam maupun hidrotermal menghasilkan ekstrak kolagen, teridentifikasi dari spektrum gugus fungsi amida I, amida II dan amida III. Kolagen yang diekstrak dengan proses asam mengandung glisin yang lebih tinggi dari proses hidrotermal, namun sebaliknya proses hidrotermal menghasilkan ekstrak dengan komposisi asam amino prolin, hidroksiprolin dan alanin yang lebih tinggi.Kata kunci: ikan cunang, isolasi, karakteristik fisikokimia, kolagen, gelembung renang Abstract Indonesian capture fisheries production reached ± 6.4 million tons in 2014. This number was predicted resulted a huge fishery waste, one of them was swim bladder that potentially contain collagen, so it needs to be developed. This aim of this study was to determine the characteristics of the swim bladder (proportion and chemical content) and collagen characteristics (yield and physicochemical properties) extracted from the swim bladder cunang (Muraenesox talabon). Swim bladder waste had proportion 0.57 to 0.67% of whole yellow-piked conger body weight and 24.74% protein/100 g wet weight (equivalent to 93.39% of protein/100 gram dry). The waste had a potency of collagen, which is characterized by the high proportion of the amino acids glycine, proline, hydroxyproline and alain. Acid extraction and hydrothermal process resulted collagen extracts, identified from a spectrum of amide I, amide II and amide III functional groups. Collagen extracted with acid process contained a higher glycine than hydrothermal one, but instead hydrothermal process produced extract with a higher proline, hydroxyproline and alanine composition.

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“…The pyrrolidine group attributes the collagen with a high thermal stability (Haugh and Draget, 2009), so heat extraction only could not optimize the extraction process. Therefore, acid pretreatment process is required to optimize the extraction process (Niu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Hydrochloric acid is a type of strong acid that could be dissociated perpectly, thus producing many hydrogen ions to break crosslinks in collagen molecules. Hydrochloric acid requires lower concentration to achieve an optimal extraction condition compared to other type of acid (Niu et al, 2013).More higher acid concentration may cause overhydrolysis of collagen molecules and losing protein during washing process (Binsi et al, 2009;Jamilah and Harvinder, 2002). The hydrolysis rate of the collagen molecules influences the yield and characteristics of gelatin.…”

Section: Introductionmentioning

confidence: 99%

The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Mulyani

Setyabudi

Pranoto

et al. 2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKTujuan penelitian adalah untuk mengidentifikasi variasi konsentrasi asam klorida terhadap yield dan sifat fisikokimia gelatin kulit kerbau. Design penelitian menggunakan Rancangan Acak Lengkap, dengan perlakuan variasi konsentrasi asam klorida 0.3M, 0.6M, 0.9 M, 1. ABSTRACTThe objective of the research was to identify the effect of the concentration of hydrochloric acid (HCl) on yield and the characteristics of gelatin from buffalo hide. A completely randomized design was used with various concentrations of HCl treatment 0.3M, 0.6M, 0.9 M, 1.2 M and 1.5M, and four times replication, followed by least significant difference test. The HCl concentration gave significantly effect (P<0.05) on yield, viscosity, pH, gel strength and redness of gelatin. The highest yield (29.17%) was obtained at a concentration of HCl 0.9M. The highest gel strength and viscosity at concentration of 1.2M, were 293.41g bloom and 22.17 cp, respectively. The pH of the gelatin were 5.08 to 5.52. The lightness level (L*) was 69.92-70.97, whereas the redness r a n g e d (a*) from 0.55 to 1.54 and the yellowness r a n g e d (b*) from 17.54 to 19.59. The moisture, protein and ash concentrations of gelatin were 7.05±0.07-8.92±0.06%, protein content of 83.38±0.06 -91.11±0.03%, and the ash content 0.53 ± 0.04 to 1.23 ± 0.09%, respectively. Based on the spectra absorption of infrared were detected four areas amides: amide A (3402.43-3448.72 cm-1), amide I (1635.65 cm-1), amide II (1527.62 cm-1) and amide III (1242.16 cm-1). The differences in the concentration of HCl had impact on yield, characteristics and secondary structure of buffalo hide gelatin. The HCl concentration at 0.9M was the optimum condition to obtain the highest yield. The characteristics of buffalo hide gelatin after pretreatment using hydrochloric acid had met the standards of GMIA (2012) .

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Characterization of tilapia (Oreochromis niloticus) skin gelatin extracted with alkaline and different acid pretreatments

Cited by 76 publications

References 31 publications

Technical note on the isolation and characterization of collagen from fish waste material

Technical note on the isolation and characterization of collagen from fish waste material

Characterization of Collagen from Swim Bladder Waste of Yellow-pike (Muraenesox talabon) by Acid and Hydrothermal Extraction

The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

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