Characterization of Toxin-Antitoxin (TA) Systems in Pseudomonas aeruginosa Clinical Isolates in Iran

Savari, Mohammad; Rostami, Soodabeh; Ekrami, Alireza; Bahador, Abbas

doi:10.5812/jjm.26627

Cited by 14 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence and type of TA systems and whether they are encoded on a plasmid or on a chromosome varies between bacteria4. TA systems are thought to be encoded on chromosomes in the P. aeruginosa 15,16. Based on bioinformatic data, four TA systems were identified on P. aeruginosa, including PAQ1, relBE, higBA, and parDE17,18.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, they also emphasized that these genes are transcribed and that the activation of toxin genes would be an effective antibacterial strategy15. In a study conducted in 2016, 174 P. aeruginosa isolates isolated from clinical specimens were found to have relBE, higBA and parDE TA genes at a rate of 100%, 100% and 30%, respectively in Iran16. Another study conducted in Iran by Hemati et al showed that P. aeruginosa isolates had mazEF TA genes at a rate of 85.7% and that the isolates were resistant to gentamicin (65%), meropenem (60%), piperacillin (59.28%), and amikacin (52.14%).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of mazEF, higBA and relBE toxin-antitoxin systems on antibiotic resistance in Pseudomonas aeruginosa and Staphylococcus isolates

et al. 2018

View full text Add to dashboard Cite

BackgroundA toxin-antitoxin (TA) system is a set of two or more closely linked genes that are encoded as a poison and a corresponding antidote on a protein. In typical bacterial physiology, an antitoxin binds to a toxin and neutralizes it, which prevents the bacterium from killing itself. We aimed to determine whether P.aeruginosa and Staphylococcus isolates have TA genes and to investigate whether there is a relationship between the expression levels of TA genes and resistance to antibiotics.MethodsThis study included 92 P. aeruginosa and 148 Staphylococcus isolates. RelBE, higBA genes were investigated in P.aeruginosa by multiplex polymerase chain reaction (PCR). The mazEF gene and the all TA genes expression were detected by real time PCR.ResultsRelBE and higBA genes were detected in 100% of P. aeruginosa. It was found that the level of relBE TA gene expression is increased in isolates sensitive to aztreonam compared to resistant isolates (p<0.05). The mazEF gene was detected in 89.1% of Staphylococcus isolates. In terms of MazEF gene expression level there was no significant difference between methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolates (p>0.05) whereas there was a significant difference between MSSA and coagulase-negative Staphylococcus (CNS) isolates, MRSA and CNS isolates (p<0.05). The levels of mazEF gene expression were found to be higher in isolates sensitive to gentamicin, ciprofloxacin, levofloxacin, clindamycin, phosphomycine, nitrofurantoin, fusidic acid, cefoxitin compared to resistant isolates (p<0.05).ConclusionStudies on the prevalence and functionality of TA systems emphasize that it may be possible to have new sensitive regions in bacteria by activating TA systems. The results of this study lead to the idea that resistance to antibiotics can be reduced by increasing TA gene expression levels. But there is need for further studies to support and develop this issue.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of mazEF, higBA and relBE toxin-antitoxin systems on antibiotic resistance in Pseudomonas aeruginosa and Staphylococcus isolates

et al. 2018

View full text Add to dashboard Cite

show abstract

“…According to researches which have been done regarding TA systems of this pathogen, it possesses YefM-yoeB, Maz EF, Hig BA, Rel BE, Par DE, Par AB, Pas TI, Tox1-Tox2, T-AT1-2, Gra TA, Vap-type system, and Hha-TomB. [51][52][53][54][55] Acinetobacter baumannii, which is increasingly becoming a significant opportunistic pathogen in nosocomial infections, is a member of the Pseudomonadales order and Moraxellaceae family and has been observed to have the following systems: Maz EF; Hig BA; Rel BE; and also prevalent Che TA systems among their species Spl TA (or Abk AB), and Gra TA. 56,57…”

Section: Gammaproteobacteria As a Significant Pathogenic Classmentioning

confidence: 99%

Modulation of Toxin-Antitoxin System Rnl AB Type II in Phage-Resistant Gammaproteobacteria Surviving Photodynamic Treatment

et al. 2018

Self Cite

View full text Add to dashboard Cite

Type II toxin-antitoxin (TA) systems are the particular type of TA modules which take part in different kinds of cellular actions, such as biofilm formation, persistence, stress endurance, defense of the bacterial cell against multiple phage attacks, plasmid maintenance, and programmed cell death in favor of bacterial population. Although several bioinformatics and Pet lab studies have already been conducted to understand the functionality of already discovered TA systems, still, more work in this area is required. Rnl AB type II TA module, which is composed of RnlA toxin and RnlB antitoxin, is a newly discovered type II TA module which takes part in the defense mechanism against T4 bacteriophage attack in Escherichia coli K-12 strain MH1 that has not been widely studied in other bacteria. Because of the significant role of class Gammaproteobacteriacea in a diverse range of health problems, we chose here to focus on this class to survey the presence of the Rnl AB TA module. For better categorization and description of the distribution of this module in this class of bacteria, the corresponding phylogenetic trees are illustrated here. Neighbor-joining and the maximum parsimony methods were used in this study to take a look at the distribution of domains present in RnlA and RnlB proteins, among members of Gammaproteobacteria. Also, the possible roles of photodynamic therapy (PDT) in providing a substrate for better phage therapy are herein discussed.

show abstract

“…Repetitive element-based PCR (Rep-PCR) has been widely used to study the strain-specific patterns obtained from PCR amplification of repetitive DNA elements present within bacterial genomes [ 9 , 10 ]. The advantages of Rep-PCR over other molecular typing methods include the ability to differentiate between closely related strains of bacteria, as well as being a simple, quick, inexpensive, and reliable high-throughput genotyping method [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of BOX-PCR and ERIC-PCR as Molecular Typing Tools for PathogenicLeptospira

et al. 2018

View full text Add to dashboard Cite

In the last decades, leptospirosis had gained public health concern due to morbidity and mortality rates caused by pathogenic Leptospira. The need for rapid and robust molecular typing methods to differentiate this zoonotic pathogen is of utmost importance. Various studies had been conducted to determine the genetic relatedness of Leptospira isolates using molecular typing methods. In this study, 29 pathogenic Leptospira isolates from rat, soil, and water samples in Sarawak, Malaysia, were characterized using BOX-PCR and ERIC-PCR. The effectiveness of these two methods with regard to the ease of interpretation, reproducibility, typeability, and discriminatory power was also being evaluated. Using BOX-PCR, six clusters and 3 single isolates were defined at a genetic distance percentage of 11.2%. ERIC-PCR clustered the isolates into 6 clusters and 2 single isolates at a genetic distance percentage of 6.8%. Both BOX-PCR and ERIC-PCR produced comparable results though the discriminatory index for ERIC-PCR (0.826) was higher than that for BOX-PCR (0.809). From the constructed dendrogram, it could be summarized that the isolates in this study were highly heterogeneous and genetically diverse. The findings from this study indicated that there is no genetic relatedness among the pathogenic Leptospira isolates in relation to the locality, source, and identity, with some exceptions. Out of the 29 pathogenic Leptospira isolates studied, BOX-PCR and ERIC-PCR successfully discriminated 4 isolates (2 isolates each) into the same cluster in relation to sample sources, as well as 2 isolates into the same cluster in association with the sample locality. Future studies shall incorporate the use of other molecular typing methods to make a more thorough comparison on the genetic relatedness of pathogenic Leptospira.

show abstract

Characterization of Toxin-Antitoxin (TA) Systems in Pseudomonas aeruginosa Clinical Isolates in Iran

Cited by 14 publications

References 19 publications

Effect of mazEF, higBA and relBE toxin-antitoxin systems on antibiotic resistance in Pseudomonas aeruginosa and Staphylococcus isolates

Effect of mazEF, higBA and relBE toxin-antitoxin systems on antibiotic resistance in Pseudomonas aeruginosa and Staphylococcus isolates

Modulation of Toxin-Antitoxin System Rnl AB Type II in Phage-Resistant Gammaproteobacteria Surviving Photodynamic Treatment

Evaluation of BOX-PCR and ERIC-PCR as Molecular Typing Tools for PathogenicLeptospira

Contact Info

Product

Resources

About