Characterization of transduction by bacteriophage T1: time of production and density of transducing particles

Kylberg, Karin; Bendig, Mary M.; Drexler, Henry

doi:10.1128/jvi.16.4.854-858.1975

Cited by 13 publications

(8 citation statements)

References 16 publications

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“…Therefore, less and less bacterial DNA is available for packaging into mature particles as maturation progresses. As a result of degradation of bacterial DNA, premature lysis has a relatively greater effect on decreasing the availability of phage DNA compared with bacterial DNA (13). (ii) Ti phage with mutations in gene 2.5 do not degrade the bacterial chromosome.…”

Section: Resultsmentioning

confidence: 99%

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T1 Genes Which Affect Transduction

Borchert

Drexler

1980

J Virol

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Amber mutants of Ti were grown on each of three donor strains which were identical except that they carried different suppressors: respectively, supD, supE, and supB. The efficiency with which the mutants were able to transduce was tested after growth on each donor. In general, it was found that functions which control the synthesis of phage DNA usually caused significant increases in the efficiency of transduction (EOT). A few mutants located in genes essential for head production caused significant decreases in EOT. The presence of a particular suppressor in a donor can cause noteworthy changes in the EOT by certain of the mutant phages. Amber mutations in gene 3 of Ti were extremely sensitive to the particular suppressor present in the donor, showing a 17-fold decrease in EOT compared with other mutants after growth in donors with the supD suppressor and a 75-fold increase after growth in supE donors. Increases in EOT by early genes of Ti do not seem to be caused by a lack of competition of bacterial DNA with phage DNA during packaging since, in most instances, infective phage were produced in relatively normal amounts compared with wild-type Ti. Phage DNA synthesis and degradations of the host chromosome are closely coupled in Ti infections; we believe that increases in EOT by mutants of early functions are due to inefficient degradation of the host chromosome.

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Section: Resultsmentioning

confidence: 99%

“…The standard media have been described in a previous report. Likewise, lysate preparation was by standard methods previously reported (6,13).…”

Section: Methodsmentioning

confidence: 99%

T1 Genes Which Affect Transduction

Borchert

Drexler

1980

J Virol

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“…(i) Integration of Mu can lead to deletions (14) that may remove these specific recognition sites. (ii) Mu DNA is about 90% the length of T1 DNA (15,18). Therefore, only recognition sites located near the prophage integration site could be used to initiate packaging of a piece of DNA that would contain the entire Mu prophage.…”

Section: Discussionmentioning

confidence: 99%

“…Equilibrium density gradient analysis. The density gradient technique has been described in detail in a previous publication (15). A brief description is given in the legend to Fig.…”

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confidence: 99%

Transduction of bacteriophage Mu by bacteriophage T1

Bendig¹,

Drexler²

1977

J Virol

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Phage T1 transduces phage Mu PFU from Mu-lysogenic donor cells to sensitive recipient cells. The efficiency of transduction depends on the chromosomal location of the Mu prophage. T1, therefore, appears to package different regions of the bacterial chromosome with different efficiencies. Although T1 transduces bacterial markers with different efficiencies, there is no direct correlation between the efficiency of transduction of a bacterial marker and the efficiency of transduction of Mu PFU from donor cells with the Mu prophage located in that marker.

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“…In an effort to ascertain the location of attP7 by transduction it was decided to use phage T1 as the transducing phage in place of P1. T1 transduces chromosomal markers in a manner apparently similar to that of P1 [7][8][9][10], the major difference being that, due to the smaller size of the T1 genome (31-32. 10 6 Mr) [11,12], the amount of chromosomal DNA transduced is considerably less than that for P1.…”

Section: Introductionmentioning

confidence: 99%

Anomalous T1 transduction of the attP7 region of Escherichia coli

Chesney

1983

FEMS Microbiology Letters

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Characterization of transduction by bacteriophage T1: time of production and density of transducing particles

Cited by 13 publications

References 16 publications

T1 Genes Which Affect Transduction

T1 Genes Which Affect Transduction

Transduction of bacteriophage Mu by bacteriophage T1

Anomalous T1 transduction of the attP7 region of Escherichia coli

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