T1 Genes Which Affect Transduction

Abstract

Amber mutants of Ti were grown on each of three donor strains which were identical except that they carried different suppressors: respectively, supD, supE, and supB. The efficiency with which the mutants were able to transduce was tested after growth on each donor. In general, it was found that functions which control the synthesis of phage DNA usually caused significant increases in the efficiency of transduction (EOT). A few mutants located in genes essential for head production caused significant decreases in EOT. The presence of a particular suppressor in a donor can cause noteworthy changes in the EOT by certain of the mutant phages. Amber mutations in gene 3 of Ti were extremely sensitive to the particular suppressor present in the donor, showing a 17-fold decrease in EOT compared with other mutants after growth in donors with the supD suppressor and a 75-fold increase after growth in supE donors. Increases in EOT by early genes of Ti do not seem to be caused by a lack of competition of bacterial DNA with phage DNA during packaging since, in most instances, infective phage were produced in relatively normal amounts compared with wild-type Ti. Phage DNA synthesis and degradations of the host chromosome are closely coupled in Ti infections; we believe that increases in EOT by mutants of early functions are due to inefficient degradation of the host chromosome.

Virulent Drexlervirial Bacteriophage MSK, Morphological and Genome Resemblance With Rtp Bacteriophage Inhibits the Multidrug-Resistant Bacteria

“…In comparison, Drexlerviridae family phage phiC119 showed a latent period of 20 min and burst size (210) 58 min ( Amarillas et al, 2016 ). In contrast, T1 bacteriophage exhibit a latent period of 13 min and produces 100 virions per infected phage ( Borchert and Drexler, 1980 ). Further, several factors like mutation, host cell size, host physiology, and culture condition might reflect the short burst size and the latent period during the propagation in the host ( Sharaf et al, 2018 ).…”

Anomalous T1 transduction of the attP7 region of Escherichia coli

“…In an effort to ascertain the location of attP7 by transduction it was decided to use phage T1 as the transducing phage in place of P1. T1 transduces chromosomal markers in a manner apparently similar to that of P1 [7][8][9][10], the major difference being that, due to the smaller size of the T1 genome (31-32. 10 6 Mr) [11,12], the amount of chromosomal DNA transduced is considerably less than that for P1.…”