Characterization of Two Thermostable β-agarases from a Newly Isolated Marine Agarolytic Bacterium, Vibrio sp. S1

Chi, Won-Jae; Seo, Ju Won; Hong, Soon-Kwang

doi:10.1007/s12257-019-0180-9

Cited by 4 publications

(1 citation statement)

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“…Macroalgae are commonly divided into three subclassifies, including green algae (Chlorophyta), brown algae (Phaeophyta), and red algae (Rhodophyta), and found in abundance along the coast in intertidal zones, sublittoral-to-littoral zones, and lighted rock or other hard substrata [ 1 ]. Agar, as a main heterogeneous hydrocolloid polysaccharide compound of red algae (such as Gelidium , Gracilaria , and Ceramium ), mainly consists of agarose and agaropectin and is commonly employed as a food additive and gelling agent for bacterial culture with a huge production of 10,600 tons per year [ 2 , 3 ]. However, the potential highly valuable bioactive of the agar of these macroalgae species has not given full play to its function related to the properties of high viscosity, low water solubility, and difficulty in being absorbed.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Antioxidant Composition of Low Molecular Weight Metabolites from the Agarolytic Bacterium Alteromonas macleodii QZ9-9: Possibilities for High-Added Value Utilization of Macroalgae

Wang

Feng

et al. 2022

Antioxidants

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Agar accounts for ~60% of the dry weight of some red macroalgae, and the breakdown of this kind of polysaccharide releases high-value compounds; therefore, the resource utilization of agar is of great significance to improve the added value of these macroalgae. Herein, Alteromonas macleodii QZ9-9 isolated from tropical Gracilaria hainanensis in Hainan Island was characterized as an agarolytic bacterium, which displayed a high agar-degrading activity. The highest diameters of the degradation zones of the A. macleodii QZ9-9 and its extracellular-agarase (12.16 U/mL) were 41.46 mm and 22.89 mm, respectively, and the first-order degradation rate constants of those were 0.02 h−1 and 0.77 U−1, respectively. Importantly, the fermentation products of A. macleodii QZ9-9 exhibited antioxidant activity, and the peak of DPPH scavenging activity of 50 h fermentation products of this strain was up to 50.79% in the reaction for 1 h; the DPPH scavenging activity of low molecule metabolites (≤3 kDa) in particular was up to ~85.85%. A total of 766 metabolites were detected in the low molecule metabolites by metabolomics. The peptide-like metabolites, such as prolyl–histidine, isoleucyl–histidine, isoleucyl–proline and arginyl–proline, and the antioxidant maculosin were found in the top 20 metabolites with relatively high abundance. Additionally, the antioxidant activity of maculosin was further verified in this work. We concluded that the low molecule metabolites of A. macleodii QZ9-9 with relatively high antioxidant activity are interesting candidates for preparing desirable non-toxic antioxidants, thereby facilitating the high value-added utilization of macroalgae in the fields of cosmetic, food preservation, and pharmaceutical industries.

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Section: Introductionmentioning

confidence: 99%