Characterization of verocytotoxin-producing<i>Escherichia coli</i>O157 isolates from patients with haemolytic uraemic syndrome in Western Europe

Heuvelink, Annet; Kar, Nicole C. A. J. van de; Meis, Jacques F.; Monnens, L.A.H.; Melchers, Willem J. G.

doi:10.1017/s0950268800058064

Cited by 84 publications

(54 citation statements)

References 45 publications

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“…The distribution of PTs in Finland was similar to that found in several other countries, especially in Europe (2,3,7,8,15). While the range of PT and PFGE types isolated from human infections in Finland indicates several domestic sources for STEC O157 infections, only one sporadic infection could be linked to a known food item, unpasteurized milk (16).…”

supporting

confidence: 83%

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157 in Finland

et al. 2001

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supporting

confidence: 83%

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157 in Finland

et al. 2001

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“…PT2 and PT8 were predominant among human and bovine STEC O157:H7 strains in Spain as well as in many other European countries, including Belgium, Finland, Germany, Italy, England, and Scotland (20,22,23,30,33,34,46,58 . According to their phage types, stx genotypes, and phenotypes, the STEC O157:H7 strains isolated in Spain were very similar to those isolated in other parts of Europe.…”

Section: Discussionmentioning

confidence: 99%

“…STEC O157:H7 PT2 stx 2 -positive and stx 2c -positive strains were significantly more frequently associated with HUS and bloody diarrhea in Finland (26). A close association was found between the presence of the stx 2 (stx 2 -positive and stx 2c -negative) variant gene and severe disease in infected humans in Germany (10), Holland (33), and Japan (51). Most HUS patients from those German and Dutch studies were infected with STEC O157:H7 PT2 or PT4 strains.…”

Section: Discussionmentioning

confidence: 99%

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157:H7 Isolates from Humans and Animals in Spain: Identification and Characterization of Two Predominating Phage Types (PT2 and PT8)

Mora

Blanco

et al. 2004

J Clin Microbiol

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Phage typing and DNA macrorestriction fragment analysis by pulsed-field electrophoresis (PFGE) were used for the epidemiological subtyping of a collection of Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains isolated in Spain between 1980 and 1999. Phage typing distinguished a total of 18 phage types among 171 strains isolated from different sources (67 humans, 82 bovines, 12 ovines, and 10 beef products). However, five phage types, phage type 2 (PT2; 42 strains), PT8 (33 strains), PT14 (14 strains), PT21/28 (11 strains), and PT54 (16 strains), accounted for 68% of the study isolates. PT2 and PT8 were the most frequently found among strains from both humans (51%) and bovines (46%). Interestingly, we detected a significant association between PT2 and PT14 and the presence of acute pathologies. A group of 108 of the 171 strains were analyzed by PFGE, and 53 distinct XbaI macrorestriction patterns were identified, with 38 strains exhibiting unique PFGE patterns. In contrast, phage typing identified 15 different phage types. A total of 66 phage type-PFGE subtype combinations were identified among the 108 strains. PFGE subtyping differentiated between unrelated strains that exhibited the same phage type. The most common phage type-PFGE pattern combinations were PT2-PFGE type 1 (1 human and 11 bovine strains), PT8-PFGE type 8 (2 human, 6 bovine, and 1 beef product strains), PT2-PFGE subtype 4A (1 human, 3 bovine, and 1 beef product strains). Nine (29%) of 31 human strains showed phage type-PFGE pattern combinations that were detected among the bovine strains included in this study, and 26 (38%) of 68 bovine strains produced phage type-PFGE pattern combinations observed among human strains included in this study, confirming that cattle are a major reservoir of strains pathogenic for humans. PT2 and PT8 strains formed two groups which differed from each other in their motilities, stx genotypes, PFGE patterns, and the severity of the illnesses that they caused.

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“…Similarly, primers 5´-CAC-AAT-CAG-GCG-TCG-CCA-GCG-CAC-TTG-CT-3´ (Forward) and 5´-TGT-TGC-AGG-GAT-CAG-TCG-TAC-GGG-GAT-GC-3´(Reverse) were used for ECStx-1 at 606-bp (Heuvelink et al 1995) and primers 5´-CCA-CAT-CGG-TGT-CTG-TTA-TTA-ACC-ACA-CC-3´…”

Section: Primers Used For Pcrmentioning

confidence: 99%

“…(Forward) and 5´-GCA-GAA-CTG-CTC-TGG-ATG-CAT-CTC-TGG-TC-3´ (Reverse) were used for ECStx-2 at 374-bp (Heuvelink et al, 1995). Agarose gel electrophoresis of PCR products PCR products were analyzed by 1.5% agarose gel electrophoresis.…”

Section: Primers Used For Pcrmentioning

confidence: 99%

Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

Akter

Majumder

Nazir

et al. 2016

J Bangladesh Agric Univ

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Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.

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Characterization of verocytotoxin-producingEscherichia coliO157 isolates from patients with haemolytic uraemic syndrome in Western Europe

Abstract: Strains isolated from different persons within one family could not be distinguished by any of these methods. The data suggest that 0157 VTEC strains are members of one clone that has become widely distributed.

Cited by 84 publications

References 45 publications

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157 in Finland

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157 in Finland

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157:H7 Isolates from Humans and Animals in Spain: Identification and Characterization of Two Predominating Phage Types (PT2 and PT8)

Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

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