Characterization of Vibrio cholerae from 1986 to 2012 in Yunnan Province, southwest China bordering Myanmar

Gu, Wenpeng; Yin, Jianwen; Yang, Jianbin; Li, Chaoqun; Chen, Yujuan; Yin, Jia; Xu, Wen; Zhao, Shiwen; Liang, Junrong; Jing, Huaiqi; Fu, Xiaoqing

doi:10.1016/j.meegid.2013.10.015

Cited by 19 publications

(12 citation statements)

References 18 publications

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“…As such, the resistance of Vibrio sp. to carbapenem has been reported by Bier et al (2015) in Germany coastal line, Gu et al (2014) in Southwest China, and Walsh et al (2011) in India. Thus, our results agree with other findings and demonstrates the misuse of carbapenem that may cause a negative impact on the clinical treatment of Vibrio infections in future.…”

Section: Discussionmentioning

confidence: 95%

“…Over the years, Carbapenem-resistant Vibrio sp. has been detected and isolated from environmental and seafood samples ( Walsh et al, 2011 ; Mandal et al, 2012 ; Gu et al, 2014 ; Bier et al, 2015 ). Recently, in Kolkata, NDM-1 producing Vibrio fluvialis strains has been isolated from diarrheal fecal samples from patients ( Chowdhury et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

et al. 2018

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Vibrio parahaemolyticus, a Gram-negative halophilic bacterium is often associated with fish and fishery products, thus causing gastroenteritis in humans upon ingestion of contaminated food. V. parahaemolyticus has become a globally well-known pathogen with yearly reported cases in many countries. This study aimed to discover the antibiotic resistance patterns of V. parahaemolyticus as well as detect Carbapenem resistant isolates from marine and freshwater fish in Selangor. A total of 240 freshwater and marine fish samples collected from wet market and supermarket in Selangor were tested for the presence of V. parahaemolyticus. All the fish samples were determined positive for V. parahaemolyticus using conventional microbiological culture-based method. The toxR gene were detected via polymerase chain reaction (PCR) in 165/240 (69%) isolates. The two-virulence factor of V. parahaemolyticus, thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh) was screened via PCR. As such, four isolates were trh+and none were tdh+. Majority of the isolates presented high resistance to ampicillin (88%), amikacin (64%), and kanamycin (50%). In addition, this study identified 19-imipenem resistant isolates isolated from freshwater and marine fish samples. Further analysis of these 19-imipenem resistant isolates revealed that the resistance toward imipenem was plasmid mediated after plasmid curing assay. The multiple antibiotics resistance index was >0.2 for 70% of the isolates. In summary, the results confirm the presence of V. parahaemolyticus in freshwater and marine fish samples in Selangor, Malaysia. To our best knowledge, this is the first report discovering the antibiotic resistant patterns and Carbapenem-resistant isolates of V. parahaemolyticus isolated from marine and freshwater fish samples in Selangor.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

et al. 2018

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“…NDM-1 carbapenemase was detected in a V. cholerae O1 El Tor Ogawa strain isolated from a 2-year old child (Mandal et al, 2012 ) as well as in V. cholerae isolated from seepage water in India (Walsh et al, 2011 ). Furthermore, increasing resistance to carbapenems was recently described among V. cholerae O1 or O139 strains isolated between 1986 and 2012 in southwest China (Gu et al, 2014 ).…”

Section: Discussionmentioning

confidence: 98%

Survey on antimicrobial resistance patterns in Vibrio vulnificus and Vibrio cholerae non-O1/non-O139 in Germany reveals carbapenemase-producing Vibrio cholerae in coastal waters

et al. 2015

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An increase in the occurrence of potentially pathogenic Vibrio species is expected for waters in Northern Europe as a consequence of global warming. In this context, a higher incidence of Vibrio infections is predicted for the future and forecasts suggest that people visiting and living at the Baltic Sea are at particular risk. This study aimed to investigate antimicrobial resistance patterns among Vibrio vulnificus and Vibrio cholerae non-O1/non-O139 isolates that could pose a public health risk. Antimicrobial susceptibility of 141 V. vulnificus and 184 V. cholerae non-O1/non-O139 strains isolated from German coastal waters (Baltic Sea and North Sea) as well as from patients and retail seafood was assessed by broth microdilution and disk diffusion. Both species were susceptible to most of the agents tested (12 subclasses) and no multidrug-resistance was observed. Among V. vulnificus isolates, non-susceptibility was exclusively found toward aminoglycosides. In case of V. cholerae, a noticeable proportion of strains was non-susceptible to aminopenicillins and aminoglycosides. In addition, resistance toward carbapenems, quinolones, and folate pathway inhibitors was sporadically observed. Biochemical testing indicated the production of carbapenemases with unusual substrate specificity in four environmental V. cholerae strains. Most antimicrobial agents recommended for treatment of V. vulnificus and V. cholerae non-O1/non-O139 infections were found to be effective in vitro. However, the occurrence of putative carbapenemase producing V. cholerae in German coastal waters is of concern and highlights the need for systematic monitoring of antimicrobial susceptibility in potentially pathogenic Vibrio spp. in Europe.

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“…Pulsed field gel electrophoresis was performed based on the PulseNet protocol for V. cholerae and procedures described previously ( Gu et al, 2014 ). The enzyme digestion for each plug was Not I 40U at 37°C for 4 h. The CHEF-Mapper (Bio-Rad) was used for electrophoresis, and the pulse time ranged from 1 to 20 s for 13 h, and 20 to 25 s for 6 h. The gel was stained using Gel Red (Biotium) and visualized using the gel imaging system (Bio-Rad, Gel Doc XR).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the prevention and control of toxic strains are more important for humans. In China, cholera was considered to be one of the most serious infectious diseases, although the incidence rate has been maintained at a relatively low level in recent years, the epidemic or outbreak still existed in few areas ( Gu et al, 2014 ). It is very important to perform the molecular typing research for toxigenic V. cholerae and clarify the variation and changes of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Comparison and Evaluation of the Molecular Typing Methods for Toxigenic Vibrio cholerae in Southwest China

Chen

et al. 2018

Front. Microbiol.

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Vibrio cholerae O1 strains taken from the repository of Yunnan province, southwest China, were abundant and special. We selected 70 typical toxigenic V. cholerae (69 O1 and one O139 serogroup strains) isolated from Yunnan province, performed the pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and MLST of virulence gene (V-MLST) methods, and evaluated the resolution abilities for typing methods. The ctxB subunit sequence analysis for all strains have shown that cholera between 1986 and 1995 was associated with mixed infections with El Tor and El Tor variants, while infections after 1996 were all caused by El Tor variant strains. Seventy V. cholerae obtained 50 PFGE patterns, with a high resolution. The strains could be divided into three groups with predominance of strains isolated during 1980s, 1990s, and 2000s, respectively, showing a good consistency with the epidemiological investigation. We also evaluated two MLST method for V. cholerae, one was used seven housekeeping genes (adk, gyrB, metE, pntA, mdh, purM, and pyrC), and all the isolates belonged to ST69; another was used nine housekeeping genes (cat, chi, dnaE, gyrB, lap, pgm, recA, rstA, and gmd). A total of seven sequence types (STs) were found by using this method for all the strains; among them, rstA gene had five alleles, recA and gmd have two alleles, and others had only one allele. The virulence gene sequence typing method (ctxAB, tcpA, and toxR) showed that 70 strains were divided into nine STs; among them, tcpA gene had six alleles, toxR had five alleles, while ctxAB was identical for all the strains. The latter two sequences based typing methods also had consistency with epidemiology of the strains. PFGE had a higher resolution ability compared with the sequence based typing method, and MLST used seven housekeeping genes showed the lower resolution power than nine housekeeping genes and virulence genes methods. These two sequence typing methods could distinguish some epidemiological special strains in local area.

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Characterization of Vibrio cholerae from 1986 to 2012 in Yunnan Province, southwest China bordering Myanmar

Cited by 19 publications

References 18 publications

Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

Survey on antimicrobial resistance patterns in Vibrio vulnificus and Vibrio cholerae non-O1/non-O139 in Germany reveals carbapenemase-producing Vibrio cholerae in coastal waters

Comparison and Evaluation of the Molecular Typing Methods for Toxigenic Vibrio cholerae in Southwest China

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