Characterization of volatile sulphur production by pathogenic and non-pathogenic strains of oral Bacteroides

Tonzetich, J.; McBride, B C

doi:10.1016/0003-9969(81)90104-7

Cited by 128 publications

(74 citation statements)

References 20 publications

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“…The fact that subjects with good oral hygiene harbour less H. pylori in their mouths can be explained by the inhibitory activity of early colonizers of dental biofilm, such as oral streptococci, against that species. Periodontopathic bacteria, including Porphyromonas gingivalis, Treponema denticola and Prevotella intermedia, produce H 2 S from L-cysteine and CH 3 SH from L-methionine (Fukamachi et al, 2005;Tonzetich & McBride, 1981). H. pylori is capable of producing H 2 S and CH 3 SH, although the production of these compounds is complicated and strain specific (Lee et al, 2006).…”

Section: Prevalence Of Oral H Pylori and Oral Malodourmentioning

confidence: 99%

“…Many of the compounds that contribute to oral malodour are volatile sulfur compounds (VSCs) such as hydrogen sulfide (H 2 S), methyl mercaptan (CH 3 SH) and dimethyl sulfide (CH 3 SCH 3 ) (Kleinberg & Westbay, 1990;Tonzetich, 1977). Periodontopathic bacteria, including Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia and Fusobacterium nucleatum, produce H 2 S and CH 3 SH (Fukamachi et al, 2005;Tonzetich & McBride, 1981). Additionally, methylamine, dimethylamine, propionic acid, butyric acid, indole, scatole and cadaverine have been reported to cause oral malodour (Goldberg et al, 1994;Kostelc et al, 1981 To diagnose halitosis, a simple classification with corresponding treatment needs has been developed (Miyazaki et al, 1999;Yaegaki & Coil, 2000), which includes the categories of genuine halitosis, pseudo-halitosis and halitophobia.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Helicobacter pylori DNA in the saliva of patients complaining of halitosis

Suzuki

Yoneda

Naito

et al. 2008

Journal of Medical Microbiology

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Helicobacter pylori infection, which causes peptic ulcers and gastric cancer, is considered a possible cause of halitosis. Recently, the oral cavity was identified as a possible H. pylori reservoir, particularly in the presence of periodontal disease, which is a cause of halitosis. The purpose of this study was to evaluate by PCR the prevalence of oral H. pylori in the saliva of subjects complaining of halitosis. Samples were obtained from 326 non-dyspeptic subjects, comprising 251 subjects with actual malodour and 75 subjects without halitosis. DNA was extracted from the samples, and the presence of H. pylori and periodontopathic bacteria including Porphyromonas gingivalis, Treponema denticola and Prevotella intermedia was examined by PCR. H. pylori was detected in 21 (6.4 %) of 326 samples. The methyl mercaptan concentration and periodontal parameters including tooth mobility, periodontal pocket depth (PPD) and occult blood in the saliva were significantly greater in the H. pylori-positive subjects. Each of the periodontopathic bacteria was also detected at a significantly higher frequency in the H. pylori-positive subjects. Among those patients with a PPD of ¢5 mm and a tongue coating score of ¡2, no difference was observed in oral malodour levels between the H. pylori-positive and -negative subjects. However, the presence of occult blood in the saliva and the prevalence of Prevotella intermedia were significantly greater in the H. pylori-positive subjects. H. pylori was detected in 16 (15.7 %) of 102 subjects with periodontitis, suggesting that progression of periodontal pocket and inflammation may favour colonization by this species and that H. pylori infection may be indirectly associated with oral pathological halitosis following periodontitis. INTRODUCTIONHelicobacter pylori is a spiral, microaerophilic, Gramnegative bacterium that colonizes the human gastrointestinal tract, primarily the stomach (Covacci et al., 1999). It is also believed to be responsible for gastritis and peptic ulcers, and is a risk factor for gastric cancer (Dunn et al., 1992; Parsonnet et al., 1991;Warren & Marshall, 1983); however, the mode of transmission of H. pylori is poorly understood. Several studies have detected H. pylori in the human oral cavity, particularly in patients with gingivitis or chronic periodontitis (Anand et al., 2006; Gebara et al., 2006;Souto & Colombo, 2008), and have thus suggested that the oral cavity is the primary extragastric reservoir for H. pylori. In contrast, other studies have failed to find evidence supporting the role of the oral cavity as a major reservoir of H. pylori (Czesnikiewicz-Guzik et al., 2004;Loster et al., 2006).Halitosis is a common problem in humans, and oral malodour is largely caused by periodontitis, tongue debris, poor oral hygiene, deep caries, inadequately fitted restorations and endodontic lesions (Rosenberg et al., 1991;Tonzetich, 1977;Yoneda et al., 2006); specifically, it is primarily the result of the microbial metabolism of amino acids in local debris (Scully et al.,...

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Section: Prevalence Of Oral H Pylori and Oral Malodourmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Helicobacter pylori DNA in the saliva of patients complaining of halitosis

Suzuki

Yoneda

Naito

et al. 2008

Journal of Medical Microbiology

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“…[1][2][3][4][5] In some studies, high levels of VSC in mouth air have been occasionally found in periodontal healthy individuals. 6,13,14 On the other hand, oral malodor has been shown to be significantly related to periodontal status in both clinical and epidemiological studies.…”

Section: Introductionmentioning

confidence: 99%

Prediction of periodontopathic bacteria in dental plaque of periodontal healthy subjects by measurement of volatile sulfur compounds in mouth air

Kishi

Ohara‐Nemoto

Takahashi

et al. 2013

Archives of Oral Biology

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Objectives:The aim of this study was to determine whether measurements of volatile sulfur compounds (VSCs) are useful to predict colonization of periodontopathic bacteria. For this purpose, we assessed the relationships among distributions of 4 species of periodontopathic bacteria in tongue coating and dental plaque, oral conditions including VSC concentration in mouth air, and smoking habit of periodontal healthy young subjects. Methods:The subjects were 108 young adults (mean age, 23.5±2.56 years) without clinical periodontal pockets. Information regarding smoking habit was obtained by interview. After VSC concentration in mouth air was measured with a portable sulfide monitor (Halimeter ® ), non-stimulated saliva flow and dental caries status were assessed, and tongue coating and dental plaque samples were collected from the subjects. The tongue coating samples were weighed to determine the amount. The colonization of Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola in both tongue coating and plaque samples was investigated using species-specific polymerase chain reaction assays.Results: Significant relationships were observed between the colonization of periodontopathic bacteria in tongue coating and plaque samples, especially that of P.gingivalis. VSC concentration showed the most significant association with colonization of P. gingivalis in both tongue coating and dental plaque. Logistic regression analysis demonstrated that the adjusted partial correlation coefficient [Exp(B)] values for VSC concentration with the colonization of P. gingivalis, P. intermedia, and T. denticola in dental plaque were 135, 35.4 and 10.4, respectively. In addition, smoking habit was also shown to 3 be a significant variable in regression models [Exp(B) = 6.19, 8.92, and 2.53, respectively].Therefore, receiver operating characteristic analysis was performed to predict the colonization of periodontal bacteria in dental plaque in the subjects divided by smoking habit. Based on our results, we found cut-off values that indicated likelihood ratios (LR) within the efficient range for positive findings in both groups. Conclusion:The present results demonstrated that measurement of VSC concentration in mouth air is a useful method to predict the presence of colonization of some periodontopathic bacteria in dental plaque.

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“…Prophyromonas gingivalis and Treponema denticola. 26 Most of the protein found in mouth is in the form of glycoprotein and sugar-feeding microbes can cleave residues from these glycoproteins, leaving naked peptides to be digested by other bacteria. 27 These bacteria proteolyse the sulphur-containing amino acids from the proteins in the saliva, shed epithelium, food debris, gingival crevicular fluid, interdental plaque, and postnasal drip thereby releasing the VSCs.…”

Section: Bacteria / Microorganismsmentioning

confidence: 99%

HALITOSIS – A Social Malady

Kukkamalla¹,

Cornelio²,

Bhat³

et al. 2014

IOSRJDMS

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Characterization of volatile sulphur production by pathogenic and non-pathogenic strains of oral Bacteroides

Cited by 128 publications

References 20 publications

Detection of Helicobacter pylori DNA in the saliva of patients complaining of halitosis

Detection of Helicobacter pylori DNA in the saliva of patients complaining of halitosis

Prediction of periodontopathic bacteria in dental plaque of periodontal healthy subjects by measurement of volatile sulfur compounds in mouth air

HALITOSIS – A Social Malady

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