Characterization of VvPAL-like promoter from grapevine using transgenic tobacco plants

Jiu, Songtao; Wang, Chen; Zheng, Ting; Liu, Zhongjie; Leng, Xiangpeng; Pervaiz, Tariq; Lotfi, Abolfazl; Fang, Jinggui; Wang, Xiaomin

doi:10.1007/s10142-016-0516-x

Cited by 13 publications

(9 citation statements)

References 67 publications

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“…Compared with untreated controls, the GUS activity of the a1 and a2 deletion promoter plants increased more significantly compared to other deletion promoter plants treated with MeJA (Fig 8). This result showed that the MeJA-responsive elements (-motif and -motif) played a crucial role in enhancing the GUS activities of a1 and a2 [19–22]. Otherwise, a1-mediated GUS activity declined significantly under low temperature treatment, while a2-mediated activity increased in response to the same stimulus.…”

Section: Discussionmentioning

confidence: 99%

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize

et al. 2019

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The anionic peroxidases play an important role in a variety of plant physiological processes. We characterized and isolated the Zmap promoter (PZmap) at the 5′ flanking region in order to better understand the regulatory mechanisms of Zmap gene expression. A series of PZmap deletion derivatives, termed a1 –a6, at positions −1694, −1394, −1138, −784, −527 and −221 from the translation start site were blended to the β-glucuronidase reporter gene. Agrobacterium-mediated transformation method was used to study each deletion construct in tobaccos. Sequence analysis showed that several cis-acting elements (MYB binding site, Box-II, a TGACG-element, a CGTCA-element and a low temperature responsive element) were located within the promoter. Deletion analysis suggested the sequence between −1,694 and −1394bp may contain cis-elements associated with GUS up regulation. The MYB binding site (-757) might act as a negative drought-responsive element. There might be repressor elements located in the region (−1,694 to −1394bp) to repress Zmap expression under 4°C. The characterized promoter would be an ideal candidate for genetic engineering for improving the resistance of maize to different stressors.

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Section: Discussionmentioning

confidence: 99%

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize

et al. 2019

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“…PavActin was used as a reference gene for RT-qPCR analyses. To determine the relative fold differences for each gene in each experiment, the Ct value of the genes was normalized to the Ct value for the reference gene, and the relative expression was calculated relative to a calibrator using the formula 2 −∆∆CT [49]. All the values shown are the mean ± SE.…”

Section: Real-time Quantitative Rt-pcr Analysismentioning

confidence: 99%

Dormancy-Associated MADS-Box (DAM) Genes Influence Chilling Requirement of Sweet Cherries and Co-Regulate Flower Development with SOC1 Gene

Wang

Gao

et al. 2020

IJMS

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Floral bud dormancy release of fruit tree species is greatly influenced by climate change. The lack of chilling accumulation often results in the occurrence of abnormal flower and low yields of sweet cherries (Prunus avium L.) in warm regions. To investigate the regulation of dormancy in sweet cherries, six DAM genes with homology to peach DAM, designated PavDAM1-6, have been identified and characterized. Phylogenetic analysis indicate that these genes are similar to DAMs in peach, apple and pear. The expression patterns of the PavDAMs in the low-chill cultivar ‘Royal Lee’ were different from that in the high-chill cultivar ‘Hongdeng’. ‘Royal Lee’ exhibits lower transcriptional level of PavDAM1 compared to ‘Hongdeng’, especially at the stage of chilling accumulation, and transcriptional levels of PavDAM4/5 were high in both cultivars during the endodormancy. Ectopic expression of PavDAM1 and PavDAM5 in Arabidopsis resulted in plants with abnormal flower and seed development, especially the PavDAM5. Higher transcriptional levels of SOC1 were observed in transgenic PavDAM1/5 lines, and ectopic expression of PavSOC1 had the similar floral phenotype. Further, protein interaction analysis demonstrated that PavDAM1/5 could interact with PavSOC1 in vivo and in vitro, which will help clarify the molecular mechanism of the flower development in sweet cherry or other fruit trees.

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“…The extraction of total RNA was carried out from grapevine samples by following the cetyltrimethylammonium bromide method (Jiu et al, 2015;Jiu et al, 2016). The concentration of total RNA was evaluated using NanoDrop (Thermo Fisher Scientific Inc., USA), and OD 260/280 ratios were close to 2.0, and OD 260/230 ratios were >2.0 for all the samples.…”

Section: Total Rna Extraction and Cdna Library Constructionmentioning

confidence: 99%

The Cytochrome P450 Monooxygenase Inventory of Grapevine (Vitis vinifera L.): Genome-Wide Identification, Evolutionary Characterization and Expression Analysis

Jiu

Wang

et al. 2020

Front. Genet.

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The cytochrome P450 (CYP) monooxygenase superfamily, belonging to heme-thiolate protein products, plays a vital role in metabolizing physiologically valuable compounds in plants. To date, CYP superfamily genes have not yet been characterized in grapevine (V. vinifera L.), and their functions remain unclear. In this study, a sum of 236 VvCYPs, divided into 46 families and clustered into nine clans, have been identified based on bioinformatics analyses in grapevine genome. The characteristics of both exon-intron organizations and motif structures further supported the close evolutionary relationships of VvCYP superfamily as well as the reliability of phylogenetic analysis. The gene number-based hierarchical cluster of CYP subfamilies of different plants demonstrated that the loss of CYP families seems to be limited to single species or single taxa. Promoter analysis elucidated various cis-regulatory elements related to phytohormone signaling, plant growth and development, as well as abiotic/biotic stress responses. The tandem duplication mainly contributed to the expansion of the VvCYP superfamily, followed by singleton duplication in grapevine. Global RNA-sequencing data of grapevine showed functional divergence of VvCYPs as diverse expression patterns of VvCYPs in various organs, tissues, and developmental phases, which were confirmed by quantitative realtime reverse transcription PCR (qRT-PCR). Taken together, our results provided valuable inventory for understanding the classification and biological functions of the VvCYPs and paved the way for further functional verification of these VvCYPs and are helpful to grapevine molecular breeding.

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Characterization of VvPAL-like promoter from grapevine using transgenic tobacco plants

Cited by 13 publications

References 67 publications

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize

Dormancy-Associated MADS-Box (DAM) Genes Influence Chilling Requirement of Sweet Cherries and Co-Regulate Flower Development with SOC1 Gene

The Cytochrome P450 Monooxygenase Inventory of Grapevine (Vitis vinifera L.): Genome-Wide Identification, Evolutionary Characterization and Expression Analysis

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