Characterization of Xanthomonas oryzae pv. oryzae Isolates from Rice Growing Regions of Southern India

Shankara, K.

doi:10.18782/2320-7051.2436

Cited by 5 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…oryzae respectively. The similar results were observed by Mondal et al, (2012): Shivalingaiah et al, (2012. These pathogenic cultures were further used for in vitro experiments.…”

Section: Zincsupporting

confidence: 82%

See 1 more Smart Citation

Green Synthesis of Zinc Nanoparticles using Pseudomonas fluorescens Extract and Their Antibacterial Activity against Xanthomonas spp.

Vinay¹,

Nargund²,

Jahagirdhar³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…oryzae respectively. The similar results were observed by Mondal et al, (2012): Shivalingaiah et al, (2012. These pathogenic cultures were further used for in vitro experiments.…”

Section: Zincsupporting

confidence: 82%

“…The present results are in agreement with Prakash and Ching (2016), wherein they reported the phytotoxicity of ZnONPs on wheat seedlings at higher concentration (>400 mg/L). Shankara et al, (2017) and Giri et al, (2011).…”

Section: Zincmentioning

confidence: 95%

Green Synthesis of Zinc Nanoparticles using Pseudomonas fluorescens Extract and Their Antibacterial Activity against Xanthomonas spp.

Vinay¹,

Nargund²,

Jahagirdhar³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…Likewise with the Levan production test in which B. glumae bacteria did not form convex and slimy colonies on Levan media while the X. oryzae bacteria formed colonies that were convex and slimy. Shankara et al, (2017) None of the test isolates showed positive arginine dehydrolase test and levan production, so that the isolates showed the character of B. glumae bacteria.…”

Section: Physiological Characteristics Of Bacterial Isolatesmentioning

confidence: 94%

The Development of Rapid, Accurate and Simple Identification Techniques for Bacterial Grain Rot (Burkholderia Glumae) on Rice

Aflaha¹,

ruddin²,

Surapati³

2018

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

Article InfoGrain Rot Disease (Bacterial Grain Rot) or Bacterial Panicle Blight becomes a serious threat to rice farmers in Indonesia. Agricultural Quarantine Office in Makassar (2016) and Pare-Pare (2017) confirmed that this disease has spread throughout the rice development area in South Sulawesi, but unfortunately Food Crops and Horticulture Protection Service has not made this disease as the main disease that should be monitored regularly. This is probably due to the difficulty of control measures as the symptoms of BG disease which in general occur only when the phase is generative and often the symptoms are confused with other pest symptoms such as blast, bacterial blight, stink bugor others. To overcome this problem, a fast, accurate, and simple method must be developed to diagnose the causal agent. To evaluate this method 8 isolates of Bulkholderia glumae were obtained from several varieties and several locations. For comparison, several pathogenic bacterial isolates in rice plants. The KOH3% test results showed that the whole isolates were negative. In the media King's B shows positive in the production of toxoflavin, catalase and negative results in the arginine dehydrolase test, levan production, and gelatin liquefaction tests. The occurrence of maceration symptoms in onion slices provides more convincing evidence for the presence of B. glumae bacteria in rice. The hypersensitive reaction test in tobacco cannot be used as a benchmark because only 5 isolates react positively while the other 3 isolates react negatively. Through a series of results the tests showed differences in the characteristics of B. glumae bacteria with bacteria that cause rice diseases such as: Xanthomonas oryzae pv oryzae (leaf blight), X. oryzae pv oryzycola (leaf streak), so that the method can be used by pest observers and students to determine quickly, accurately, and simple for the presence of B. glumae bacteria in rice. The accuracy of the system will then be confirmed through molecular testing with specific primers.

show abstract

“…Karnataka state map showing the distribution of different pathotypes of Xoo in distinct districts of Karnataka Plant Protection Science, 59, 2023 (1): 31-47 https://doi.org/10.17221/76/2022-PPS and stored at 4 °C until the pathogen was isolated. The pathogen was isolated from the symptomatic leaves as described previously (Shankara et al 2017). Three single colonies were isolated from each infected leaf sample, and one strain per field was selected as a representative strain for this study.…”

Section: Pdi =mentioning

confidence: 99%

Genetic diversity and pathotype profiling of Xanthomonas oryzae pv. oryzae isolates from diverse rice growing ecosystems of Karnataka state of India

Raghunandana¹,

Pramesh²,

Sunkad³

et al. 2023

Plant Prot. Sci.

View full text Add to dashboard Cite

Bacterial leaf blight (BLB) disease of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the significant constraints for rice production. A study was carried out to analyze the virulence, genetic diversity, and population structure of Xoo isolates collected from different rice ecosystems in Karnataka, India. The taxonomic identity of all 54 isolates was confirmed using a Xoo-specific PCR assay. The virulence assay on a set of twenty-eight near-isogenic differentials identified nine pathotype groups (KPthX-1 to KPthX-9). Among them, KPthX-6 was highly virulent, whereas KPthx-2 was less virulent. Differentials with four and five resistance (R) genes (Xa4 + xa5 + xa13 + Xa21, Xa4 + Xa7 + xa13 + Xa21, and Xa4 + xa5 + Xa7 + xa13 + Xa21) were effective against all the pathotypes. Further, genetic diversity was deduced using a universal marker set, JEL-1 and JEL-2, complementary to a conserved repetitive sequence IS1112. The amplicon size and numbers varied from 100 bp to 3 Kbp and 1 to 25. Based on the amplicon counts, Jaccard's co-efficient and phylogenetic analyses were carried out and categorized the 54 isolates into three clusters (I to III). Cluster II contains the maximum number of isolates ( 27), followed by cluster I (23 isolates) and cluster III (one isolate). The structure analysis categorized the isolates into five subgroups, viz SG1, SG2, SG3, SG4, and SG5 indicate the existence of five subpopulations. The present study has identified the genetic and virulence diversity of the Xoo population and reported the effective R gene/s for different rice ecosystems, which can be used in the breeding program for the eco-specific management of BLB of rice.

show abstract

Characterization of Xanthomonas oryzae pv. oryzae Isolates from Rice Growing Regions of Southern India

Cited by 5 publications

References 5 publications

Green Synthesis of Zinc Nanoparticles using Pseudomonas fluorescens Extract and Their Antibacterial Activity against Xanthomonas spp.

Green Synthesis of Zinc Nanoparticles using Pseudomonas fluorescens Extract and Their Antibacterial Activity against Xanthomonas spp.

The Development of Rapid, Accurate and Simple Identification Techniques for Bacterial Grain Rot (Burkholderia Glumae) on Rice

Genetic diversity and pathotype profiling of Xanthomonas oryzae pv. oryzae isolates from diverse rice growing ecosystems of Karnataka state of India

Contact Info

Product

Resources

About