Characterizing clinical isolates of Acanthamoeba castellanii with high resistance to polyhexamethylene biguanide in Taiwan

Huang, Fu Chin; Shih, Ming Chang; Chang, Kai Fei; Huang, Jian; Shin, Jyh Wei; Lin, Wei Chen

doi:10.1016/j.jmii.2015.10.011

Cited by 32 publications

(38 citation statements)

References 22 publications

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“…To characterize the potential PHMB resistance mechanisms of Acanthamoeba and to develop a more effective treatment for AK, we isolated the clinical strains NCKH_D with high PHMB tolerance and created the platforms for screening and elucidating the efficacy of treatment (Huang et al., 2015). To confirm that NCKH_D does not depend on transforming to a cyst to escape the drug, we used a differential interference contrast microscope to observe the morphology of NCKH_D.…”

Section: Resultsmentioning

confidence: 99%

“…Further, the treatment is not entirely effective against all strains/isolates. Our previous work presented a PHMB highly tolerant isolate, NCKH_D, which does not depend on transforming to a cyst to escape the drug (Huang et al., 2015). However, the action and resistance mechanisms of PHMB in Acanthamoeba cells are still not clear.…”

Section: Discussionmentioning

confidence: 99%

“…Trophozoites are harvested at the logarithmic growth phase after cultivation for 3–5 days. The clinical isolates were isolated from the corneal ulcers of patients who were diagnosed with AK in the Cheng Kung University Hospital (Li et al., 2012), including the high PHMB tolerance pathogenic Acanthamoeba castellanii isolates NCKU_D which is used in this study (Huang et al., 2015). …”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we have shown that A. castellanii clinical isolates NCKH_B and NCKH_D have higher tolerance toward PHMB than does the standard strain, ATCC 30010 (Huang et al., 2015). These two isolates differ in their response to PHMB stress: NCKH_B transforms into a pseudocyst to escape the drug, but NCKH_D does not.…”

Section: Introductionmentioning

confidence: 99%

“…To gain a deeper understanding of the mechanism underlying the first step of resistance to PHMB in Acanthamoeba , we established an RNA-seq database for Acanthamoeba , including the non-pathogenic ATCC strain and the highly PHMB-tolerant Acanthamoeba castellanii strain, NCKH_D (Huang et al., 2015). In our previous study, we have shown that A. castellanii clinical isolates NCKH_B and NCKH_D have higher tolerance toward PHMB than does the standard strain, ATCC 30010 (Huang et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage

Huang

Liu

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

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Acanthamoeba castellanii is a free-living protozoan pathogen capable of causing a blinding keratitis and fatal granulomatous encephalitis. Current treatment generally involves an hourly application of polyhexamethylene biguanide (PHMB) over a period of several days but this is not entirely effective against all strains/isolates. The tolerance mechanisms of PHMB in Acanthamoeba cells remain unclear. In this study, we found that the mRNA expression level of disulfideisomerase domain containing protein (PDI) increased rapidly in surviving cells of the highly PHMB-tolerant Acanthamoeba castellanii strain, NCKH_D, during PHMB treatment, but not in the ATCC standard strain. After PDI-specific silencing, NCKH_D was found to be more vulnerable to PHMB treatment. The results described above show that PDI is an important gene for PHMB tolerance ability in a highly PHMB-tolerant strain of Acanthamoeba and provide a new insight for more efficient medicine development for Acanthamoeba keratitis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage

Huang

Liu

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

Self Cite

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Isolation and genotyping of Acanthamoeba strains (T4, T9, and T11) from amoebic keratitis patients in Iran

et al. 2016

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The continuous increase in Acanthamoeba keratitis, a severe corneal infection, worldwide is mainly due to the increase in the number of soft contact lens users. In the present study, which involves a 5-year study, a total of 138 corneal scraps and contact lenses together with their paraphernalias were obtained from suspected amoebic keratitis patients. All samples were cultured using culture-enrichment method. Pathogenic assay, using thermotolerance and osmotolerance tests were also performed on the positive strains. Sequencing of the isolated strains was done by targeting the DF3 region of 18s rRNA gene. The results revealed that 18 (13 %) of patients were infected with Acanthamoeba spp. As expected, T4 genotype was the most common genotype among the clinical samples; however, in three cases, Acanthamoeba belonging to T11 and T9 were detected. Interestingly, T9 genotype, commonly classified as non-pathogenic amoebae, was identified as a causal agent of a patient with amoebic keratitis. From the pathogenic assay, four strains belonging to T4 genotypes were highly pathogenic. This is the first report of Acanthamoeba T9 genotypes isolated in Iran and the first report of T9 type occurring in amoebic keratitis patients worldwide. Due to the increasing trend of amoebic keratitis (AK) and the identification of new genotypes, such as T9 as the causative agent of AK, more researches in this field are necessary in the region and the world at large.

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Comparative proteomic analysis of extracellular secreted proteins expressed by two pathogenic Acanthamoeba castellanii clinical isolates and a non-pathogenic ATCC strain

Huang

Lin

et al. 2016

Experimental Parasitology

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Characterizing clinical isolates of Acanthamoeba castellanii with high resistance to polyhexamethylene biguanide in Taiwan

Abstract: Our results confirm the existence of clinical isolates of A. castellanii with high resistance to PHMB in Taiwan and present the alternative drug tolerance of A. castellanii in addition to the transformation of pseudocyst/cyst.

Cited by 32 publications

References 22 publications

The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage

The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage

Isolation and genotyping of Acanthamoeba strains (T4, T9, and T11) from amoebic keratitis patients in Iran

Comparative proteomic analysis of extracellular secreted proteins expressed by two pathogenic Acanthamoeba castellanii clinical isolates and a non-pathogenic ATCC strain

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