Characterizing diabetic wound metabolism and microstructure using multi-photon microscopy

Abstract

Through two-photon excited fluorescence and second harmonic generation imaging, quantitative optical biomarkers based on endogenous sources of contrast reveal differences in the structure and metabolic function of diabetic and non-diabetic wounds.

Evaluating Cell Metabolism Through Autofluorescence Imaging of NAD(P)H and FAD

“…Recent work has also demonstrated distinct temporal and spatial patterns of skin wound healing through TPEF and SHG imaging ( Fig. 6) (43,73). An overall lower redox ratio was found in the proliferative epidermis at the wound edge (0.559 -0.058) compared to noninjured regions (0.678 -0.024), and delayed healing in diabetic wounds was evident by a significantly lower redox ratio in the epidermis at later postwound time points (74).…”