Characterizing dissolved organic matter fluorescence with parallel factor analysis: a tutorial

Stedmon, Colin; Bro, Rasmus

doi:10.4319/lom.2008.6.572b

Cited by 370 publications

(471 citation statements)

References 8 publications

Supporting

Mentioning

465

Contrasting

Unclassified

Order By: Relevance

“…The five modeled PARAFAC components were drawn from previously documented PARAFAC results that have been associated with different OM pools and compared with modeled compounds (table 2) (McKnight and others, 2001;Stedmon and others, 2003;Coble, 2007;Hudson and others, 2007;Stedmon and Bro, 2008;Cory and others, 2010). Component 1 (C1) is correlated strongly DOC concentration in this study.…”

Section: Parallel Factor Analysis Resultsmentioning

confidence: 99%

“…[Description: Likely sources, and correlation to DOC concentrations are from McKnight and others (2001), Stedmon and others (2003), Coble (2007), Hudson and others (2007), Stedmon and Bro (2008), and Cory and others (2010) …”

Section: Principal Component Analysis Resultsmentioning

confidence: 99%

“…Lastly, the EEM was normalized to the area under the water-Raman curve at an excitation wavelength of 350 nm converting the arbitrary units to Raman units (Stedmon and others, 2003;Murphy and others, 2010). The corrected EEMs then were imported into MATLAB ® (version R2015.b) for further analysis, including removing interference from Raleigh scattering, trimming and interpolating the region of the EEM not affected by scattering and/or particles, converting the data into vectors, selecting characteristic peak signals (for example, SUVA, fDOM) and ratios (HIX, FI) for further analysis based on documented literature (Coble, 2007;Hudson and others, 2007;Stedmon and Bro, 2008), and plotting the data into contour and surface maps. Four descriptors were derived from optical absorbance and fluorescence measurements:…”

Section: Optical Analysismentioning

confidence: 99%

“…Component percentages can be calculated by dividing each component loading by the sum of the component loadings to reveal qualitative differences between samples (Andersson and Bro, 2000). Procedures established by Stedmon and Bro (2008) were used to develop a PARAFAC model for the Lost River samples using the N-way toolbox, version 6.1 (Bro, 1997;Andersson and Bro, 2000) and the drEEM toolbox, version 0.1.0 (Murphy and others, 2013) with MATLAB ® (version R2015.b). The model included a non-negative constraint to help alleviate issues with higher relative instrument noise in samples having low fluorescence.…”

Section: Parallel Factor Analysismentioning

confidence: 99%

“…Additionally, the data were normalized to the unit variance to alleviate collinearity. The PARAFAC model was validated using a combination of outlier identification, residual analysis, component validation, and replication by split-half analysis (Stedmon and Bro, 2008;Murphy and others, 2013).…”

Section: Parallel Factor Analysismentioning

confidence: 99%

See 4 more Smart Citations

Characteristics of dissolved organic matter in the Upper Klamath River, Lost River, and Klamath Straits Drain, Oregon and California

Goldman¹,

Sullivan²

2017

Open-File Report

View full text Add to dashboard Cite

Section: Parallel Factor Analysis Resultsmentioning

confidence: 99%

Section: Principal Component Analysis Resultsmentioning

confidence: 99%

Section: Optical Analysismentioning

confidence: 99%

Section: Parallel Factor Analysismentioning

confidence: 99%

Section: Parallel Factor Analysismentioning

confidence: 99%

See 3 more Smart Citations

Characteristics of dissolved organic matter in the Upper Klamath River, Lost River, and Klamath Straits Drain, Oregon and California

Goldman¹,

Sullivan²

2017

Open-File Report

View full text Add to dashboard Cite

Absorption and fluorescence of dissolved organic matter in the waters of the Canadian Arctic Archipelago, Baffin Bay, and the Labrador Sea

Guéguen

Cuss

Cassels

et al. 2014

J. Geophys. Res. Oceans

View full text Add to dashboard Cite

The optical properties of dissolved organic matter (DOM) were investigated for the first time in the Canadian Arctic Archipelago (CAA), Baffin Bay (BB), and Labrador Sea (LS) as part of the International Polar Year Canada's Three Oceans project (C3O). The dynamics and composition of absorbing DOM (i.e., colored DOM, or CDOM) and fluorescent DOM (FDOM) were evaluated in several distinct water masses occupying the three regions: surface waters, Arctic outflow waters, West Greenland Intermediate waters (WGIW), upper Labrador Sea waters (uLSW), and Bottom Baffin Deep Water (BBDW). Four fluorescent components were identified by applying parallel factor analysis (PARAFAC) to 522 excitation emission matrix (EEM) spectra: three humic-like and one protein-like. The FDOM in surface waters of the CAA and BB differed considerably in character from those of the LS, with higher fluorescence intensity in the former. The fluorescence intensities of the two terrestrial humic-like components (C1 and C3) were linearly correlated with apparent oxygen utilization (AOU) in the CAA Arctic outflow and the WGIW whereas only humic-like C3 was significantly correlated with AOU in uLSW. These findings suggest that the humic-like components were produced in situ as organic matter was bio-oxidized. The slopes of the linear relationship between humic-like intensity and AOU were significantly greater in the WGIW relative to the Arctic outflow waters, which implies that FDOM in the Arctic-derived deeper layer was less prone to mineralization.

show abstract

Variations in high‐latitude riverine fluorescent dissolved organic matter: A comparison of large Arctic rivers

2013

Self Cite

View full text Add to dashboard Cite

[1] Given the pace of climate change, it is important to better understand dissolved organic matter (DOM) storage and cycling in high-latitude rivers and its subsequent export to the Arctic Ocean. To address this concern, excitation/emission matrix spectroscopy and parallel factor analysis (PARAFAC) coupled with optical indices were used to characterize the optical properties of fluorescent DOM (FDOM) and colored DOM (CDOM) in five large Arctic rivers over two seasonal cycles. Five PARAFAC components were identified and proved useful indicators for the quantitative and qualitative descriptions of DOM sources and modification processes within Arctic watersheds. In comparing Pan-Arctic relationships between the optical properties and chemical properties of DOM, three components traced terrigenous biomarkers and two components are introduced as potential indicators for microbial processing. Conversely, no individual PARAFAC component could be directly linked to a specific plant source or river and simpler absorbance indices (i.e., a 350 ) proved to be better suited to quantify dissolved organic carbon and lignin phenol concentrations. Differences in FDOM character between the rivers could be explained by general watershed characteristics, including vegetation, topography, and hydrology. Based on these differences, the influence of coniferous vegetation on DOM character is most apparent in the Lena and Yenisei Rivers and bog-derived FDOM sources are more important in the Ob River. This study illustrates how increased hydrological connectivity in the Ob catchment and the abundance of lakes within the Mackenzie watershed may influence FDOM concentrations and the microbial processing of DOM within these watersheds.

show abstract

Characterizing dissolved organic matter fluorescence with parallel factor analysis: a tutorial

Cited by 370 publications

References 8 publications

Characteristics of dissolved organic matter in the Upper Klamath River, Lost River, and Klamath Straits Drain, Oregon and California

Characteristics of dissolved organic matter in the Upper Klamath River, Lost River, and Klamath Straits Drain, Oregon and California

Absorption and fluorescence of dissolved organic matter in the waters of the Canadian Arctic Archipelago, Baffin Bay, and the Labrador Sea

Variations in high‐latitude riverine fluorescent dissolved organic matter: A comparison of large Arctic rivers

Contact Info

Product

Resources

About