Characterizing skewed chromatographic band broadening

Yau, Wallace W.

doi:10.1021/ac50011a017

Cited by 123 publications

(41 citation statements)

References 12 publications

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“…To simulate tailing the Gaussian function in Eq. (15) can be modified by multiplication with an exponential function [34][35][36][37][38]. For practical reasons, the concentration profile c i is fragmented in arbitrary intervals, which are then replaced by distribution curves using the migration times t A R and t B R and the mean theoretical plate number N AB (N AB 5 N A 1N B )/2).…”

Section: The Smmentioning

confidence: 99%

Investigation of the stereodynamics of molecules and catalyzed reactions by CE

Trapp

2010

Electrophoresis

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The investigation of the molecular dynamics of stereoisomers and the study of the kinetics of reactions, in particular of catalyzed reactions, is of fundamental interest in chemistry, biochemistry, and medicine. Understanding how to control the transition state of a reaction allows for a directed design of new catalysts and benign processes. The integration of reactions and capillary or microchip-based electrophoretic separations is highly attractive to perform on-column derivatizations or enzymatic on-column digests of peptides and proteins for further characterization. The present review article focuses on the recent advances to study the stereodynamics of molecules and reaction kinetics of catalyzed processes by means of CE. Models and algorithms to evaluate interconversion profiles obtained by electrophoretic separation techniques are discussed with respect to the challenging demands of high separation efficiencies typical for electrophoretic techniques. Models used for evaluation are based on iterative computer simulation algorithms using the theoretical plate model or stochastic model of chromatography, empirical calculation methods, derived from equations used in chemical engineering, namely Damköhler analysis, and direct access with the approximation function and more recently with the unified equation, which can be applied to all kinds of first-order reactions taking place during a chromatographic or a electrophoretic separation. Furthermore, areas of applications are presented and discussed to give a guideline for using dynamic CE and on-column reaction electrophoresis to study kinetics of reactions and dynamic processes.

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Section: The Smmentioning

confidence: 99%

Investigation of the stereodynamics of molecules and catalyzed reactions by CE

Trapp

2010

Electrophoresis

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“…where A is the area of the peak (zeroth moment), t time and C the concentration at any time t [21]. In using Eq.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a cyano stationary phase for the determination of tacrolimus, sirolimus and cyclosporin A in whole blood by high-performance liquid chromatography–tandem mass spectrometry

Hatsis

Volmer

2004

Journal of Chromatography B

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“…This is particularly true if the chromatographic peak is close to symmetric [35,37,38]. On the other hand, the second statistical moment (M 2 ) represents the variance of the probability density distribution curve and also describes the peak width [37,38]. Finally, the third statistical moment (M 3 ) quantifies the peak asymmetry.…”

Section: Statistical Moment Descriptorsmentioning

confidence: 99%

Diffusion and Equilibrium Adsorption Coefficients of Aromatic Hydrocarbon Species in Capillary Columns

Navarro-Tovar

Moreira

Valadés-Pelayo

et al. 2014

International Journal of Chemical Reactor Engineering

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This study focuses on a mathematical description of elution of aromatic species in a 30 m Â 0.25 mm Â 0.25 μm BPX5 capillary column. Experimental studies involve the detection of chemical species at the capillary column outlet. Using the recorded peaks, statistical moments of different orders are calculated for both toluene and naphthalene. Modeling studies involve a laminar flow model with an equilibrium coefficient (K s ) and a solute diffusion coefficient in the stationary phase (D s ). Model validation is developed by performing experiments at different carrier gas velocities and GC oven temperatures. Under the conditions, neither the first statistical moment (M 1 ) nor the second statistical moment (M 2 ) is affected by the carrier gas velocity. Thus, the interface equilibrium coefficients (K s ) and the diffusion coefficients (D s ) solely depend on the solute and the capillary column polymer coating properties. Furthermore, the statistical moments of different orders show that the proposed interface equilibrium based model in the "narrow bore" 30 m Â 0.25 mm Â 0.25 μm BPX5 capillary column is adequate for aromatic species such as toluene and naphthalene.

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Characterizing skewed chromatographic band broadening

Cited by 123 publications

References 12 publications

Investigation of the stereodynamics of molecules and catalyzed reactions by CE

Investigation of the stereodynamics of molecules and catalyzed reactions by CE

Evaluation of a cyano stationary phase for the determination of tacrolimus, sirolimus and cyclosporin A in whole blood by high-performance liquid chromatography–tandem mass spectrometry

Diffusion and Equilibrium Adsorption Coefficients of Aromatic Hydrocarbon Species in Capillary Columns

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