Characterizing the cultivar-specific mechanisms underlying the accumulation of quality-related metabolites in specific Chinese tea (Camellia sinensis) germplasms to diversify tea products

Li, Jianlong; Xiao, Yao; Zhou, Xiaochen; Liao, Yinyin; Wu, Shuhua; Chen, Jiaming; Qian, Jiajia; Yan, Ying; Tang, Jinchi; Zeng, Lanting

doi:10.1016/j.foodres.2022.111824

Cited by 14 publications

(7 citation statements)

References 135 publications

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“…Tea (Camellia sinensis) is a perennial woody plant and an economically important crop in China (Li et al, 2022). The secondary metabolites of tea, such as tea polyphenols, theophylline, organic acids, can excite and relieve fatigue, detoxify and quench thirst, diuresis and improve eyesight, supplement nutrition, etc.…”

Section: Introductionmentioning

confidence: 99%

Distribution pattern of endophytic bacteria and fungi in tea plants

et al. 2022

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Endophytes are critical for plant growth and health. Tea is an economically important crop in China. However, little is known about the distribution pattern and potential functions of endophytic communities in tea trees. In this study, two genotypes (BXZ and MF) cultivated under the same conditions were selected, and endophytic bacteria and fungi were analyzed through 16S rRNA and ITS high-throughput sequencing technologies, respectively. For endophytic bacteria, root tissues harbored the most diverse endophytes, followed by stems and old leaves, and new leaves possessed the lowest diversity. In contrast, old leave tissues harbored more diverse endophytic fungi than did root and stem tissues. Most of the dominant endophytes showed obvious cultivar and tissue preferences. Tissue type played a more important role in shaping community structure than did cultivar. Nevertheless, some endophytic bacterial groups, which mainly affiliated to Chryseobacterium, Sphingomonas, Rhizobium, Morganella, Methylobacterium and Comamonadaceae, could parasitize different tissues, and the average relative abundance of endophytic bacteria was as high as 72.57%. Some endophytic fungal populations, such as Colletotrichum, Uwebraunia, Cladosporium, and Devriesia, could also parasitize tea, and the relative abundance accounted for approximately 25.70–97.26%. The cooperative relationship between endophytic bacteria and fungi in the new leaves was stronger than that in the old leaves, which can better participate in the metabolism of tea material.

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Section: Introductionmentioning

confidence: 99%

Distribution pattern of endophytic bacteria and fungi in tea plants

et al. 2022

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“…Notably, catechin and epicatechin were the most abundant compounds observed. As essential monomers for the synthesis of PAs, catechin and epicatechin are widely distributed in plants, especially in tea, where they account for 12% – 24% of the plant dry weight [38] .…”

Section: Resultsmentioning

confidence: 99%

Sustainable conversion of cottonseed hulls to valuable proanthocyanidins through ultrasound-assisted deep eutectic solvent extraction

Cui,

Fang,

Tian

et al. 2023

Ultrasonics Sonochemistry

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“…Taking the pre-treatment step together, the extraction procedures were fully investigated. The concentration of target compounds was significantly different in the two species, which probably because of the tea plant was a metabolism-abundant species [ 22 ]. This proposed analytical procedure will benefit not only the functional validation of magnesium chelatase subunits but the research of chloroplast-nucleus retrograde signaling, which is essential for discovering the chloroplast biogenesis and coordination of the expression of nuclear and chloroplast genes [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS

Zhang

Zhu

et al. 2023

Plant Methods

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Backgrounds Insertion of Mg2+ into protoporphyrin IX (PPIX) to produce magnesium-protoporphyrin IX (Mg-PPIX) was the first step toward chlorophyll biosynthesis, which not only imparts plants green pigmentation but underpins photosynthesis. Plants that blocked the conversion of PPIX to Mg-PPIX displayed yellowish or albino-lethal phenotypes. However, the lack of systematic study of the detection method and the metabolic difference between species have caused the research on chloroplast retrograde signaling controversial for a long time. Results An advanced and sensitive UPLC-MS/MS strategy for determining PPIX and Mg-PPIX was established in two metabolic different plants, Arabidopsis thaliana (Columbia-0) and Camellia sinensis var. sinensis. Two metabolites could be extracted by 80% acetone (v/v) and 20% 0.1 M NH4OH (v/v) without hexane washing. Since the Mg-PPIX could be substantially de-metalized into PPIX in acidic conditions, analysis was carried out by UPLC-MS/MS with 0.1% ammonia (v/v) and 0.1% ammonium acetonitrile (v/v) as mobile phases using negative ion multiple reaction monitoring modes. Interestingly, it could be easier to monitor these two compounds in dehydrated samples rather than in fresh samples. Validation was performed in spiked samples and mean recoveries ranged from 70.5 to 916%, and the intra-day and inter-day variations were less than 7.5 and 10.9%, respectively. The limit of detection was 0.01 mg·kg− 1 and the limit of quantification was 0.05 mg·kg− 1. The contents of PPIX (1.67 ± 0.12 mg·kg− 1) and Mg-PPIX (3.37 ± 0.10 mg·kg− 1) in tea were significantly higher than in Arabidopsis (PPIX: 0.05 ± 0.02 mg·kg− 1; Mg-PPIX: 0.08 ± 0.01 mg·kg− 1) and they were only detected in the leaf. Conclusions Our study establishes a universal and reliable method for determining PPIX and Mg-PPIX in two plants using UPLC-MS/MS. This procedure will facilitate studying chlorophyll metabolism and natural chlorophyll production.

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Characterizing the cultivar-specific mechanisms underlying the accumulation of quality-related metabolites in specific Chinese tea (Camellia sinensis) germplasms to diversify tea products

Cited by 14 publications

References 135 publications

Distribution pattern of endophytic bacteria and fungi in tea plants

Distribution pattern of endophytic bacteria and fungi in tea plants

Sustainable conversion of cottonseed hulls to valuable proanthocyanidins through ultrasound-assisted deep eutectic solvent extraction

Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS

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