2006
DOI: 10.1016/j.micron.2005.03.014
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Characterizing the topography of membrane receptors and signaling molecules from spatial patterns obtained using nanometer-scale electron-dense probes and electron microscopy

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Cited by 88 publications
(109 citation statements)
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“…The required high resolution to visualize the constituents of protein complexes is achieved by transmission electron microscopy (TEM). However, conventional TEM of whole cells is limited by the requirement of thin samples/sections slicing through the plasma membrane 27 , or plasma membrane ripping or breaking off 28,29 resulting in randomly formed small pieces of membrane. The plasma membrane is thus not imaged as a whole, leading to a lack of possibly crucial cellular context information.…”
Section: Representative Resultsmentioning
confidence: 99%
“…The required high resolution to visualize the constituents of protein complexes is achieved by transmission electron microscopy (TEM). However, conventional TEM of whole cells is limited by the requirement of thin samples/sections slicing through the plasma membrane 27 , or plasma membrane ripping or breaking off 28,29 resulting in randomly formed small pieces of membrane. The plasma membrane is thus not imaged as a whole, leading to a lack of possibly crucial cellular context information.…”
Section: Representative Resultsmentioning
confidence: 99%
“…Digital TEM images were analyzed with a FIJI (ImageJ) plugin (Particle Picker) customized to find and count coordinates of two sizes of gold particles (Zhang et al, 2006). Ripley's K bivariate function was utilized to evaluate co-clustering using the gold code for MATLAb (Wilson et al, 2004;Zhang et al, 2006).…”
Section: Discussionmentioning
confidence: 99%
“…Secondary electron and BSE images were collected simultaneously by corresponding detectors. To analyze the surface distribution of LAMP1, the x-y coordinates of the gold particles were determined using an ImageJ plug-in (Zhang et al, 2006). Ripley's k function was calculated using the coordinates and then standardized to the 99% confidence interval of a random pattern (Zhang et al, 2006).…”
Section: Methodsmentioning
confidence: 99%