Charge-balanced biphasic electrical stimulation inhibits neurite extension of spiral ganglion neurons

Shen, Na; Liang, Qiong; Liu, Yuehong; Lai, Bin; Li, Wen; Wang, Zhengmin; Li, Shufeng

doi:10.1016/j.neulet.2016.04.069

Cited by 14 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the evidence from organotypic cultures pointed out that the primary afferent nerve terminals and/or the ribbon synapses (each “type‐I” SGN is connected with a single IHC by a single ribbon synapse) were likely the sites of damage (Kopelovich et al, ). Consistent with above‐described findings, our previous study has also demonstrated that charge‐balanced biphasic electrical stimulation inhibited neural extension of SGNs in vitro (Shen et al, ). Thus, it is possible that, although electrical stimulation is safe and effective, it also induces structural changes in the synapses that affect the overall function of the system.…”

Section: Introductionsupporting

confidence: 88%

Electrical stimulation induces synaptic changes in the peripheral auditory system

Zhang

et al. 2019

J of Comparative Neurology

Self Cite

View full text Add to dashboard Cite

Since a rapidly increasing number of neurostimulation devices are used clinically to modulate specific neural functions, the impact of electrical stimulation on targeted neural structure and function has become a key issue. In particular, the specific effect of electrical stimulation via a cochlear implant (CI) on inner hair cell (IHC) synapses remains unclear. Importantly, CI candidacy has recently expanded to include patients with partial hearing loss. Unfortunately, some CI recipients experience progressive hearing loss after activation of electrical stimulation. The mechanism(s) accounting for loss of residual hearing following electrical stimulation is unknown. Here normalhearing guinea pigs were implanted with customized CIs. Intracochlear electrical stimulation with an intensity equal to or above electrically evoked compound action potential (ECAP) threshold decreased the excitability of auditory nerve. Furthermore, the number of synapses between IHCs and the afferent spiral ganglion neurons (SGNs) also decreased after electrical stimulation with higher intensities. However, no significant change was observed in the packing density and perikaryal area of SGNs as well as the quantity of hair cells. These results carry important implications for use of CIs in patients with residual hearing and for an increasing number of patients treated with other neurostimulation devices. Notably, the results were based on acute electrical stimulation. Considering the complex interaction between CIs and targeted tissues, it is urgent to conduct further research to clarify whether the similar changes could be induced by chronic electrical stimulation.

show abstract

Section: Introductionsupporting

confidence: 88%

Electrical stimulation induces synaptic changes in the peripheral auditory system

Zhang

et al. 2019

J of Comparative Neurology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The continuous electrical stimulation may cause excessive Ca 2+ influx and lead to toxic effect which induces SGN death [ 27 ]. This was verified by our previous study [ 26 ] that charge-balanced biphasic electrical stimulation inhibited the neurite growth of SGNs and decreased Schwann cell density in vitro. But the effect of cochlear implant-based electrical stimulation on the electrophysiological characteristics, the base of neuronal signal transmission, of spiral ganglion neurons is unclear.…”

Section: Introductionsupporting

confidence: 83%

“…Calcium overload and oxidative stress are the main causes for SGNs death [ 27 ] and the important mechanisms of delayed neuronal death [ 35 ]. Our previous study found that charge-balanced biphasic electrical stimulation inhibited the neurite growth of SGNs and decreased Schwann cell density in vitro, and calcium influx through multiple types of VDCCs was involved in the electrical stimulation-induced neurites growth inhibition in SGNs [ 26 ]. In this study, we found that I Ca was significantly reduced after 48 h electrical stimulation in both 50 μ A and 100 μ A group.…”

Section: Discussionmentioning

confidence: 99%

“…Like the noise-induced deafness, the main mechanisms of the noise-induced deafness are the glutamate excite-toxicity, calcium overload, and oxidative stress [ 23 – 25 ]. Electrical stimulation can both induce voltage-gating calcium channels (VDCCs) opening, and the calcium influx and multiple types of VDCCs are involved in the neurite growth inhibition of SGNs [ 26 ] induced by the electrical stimulation. The continuous electrical stimulation may cause excessive Ca 2+ influx and lead to toxic effect which induces SGN death [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background. Cochlear implant-based electrical stimulation may be an important reason to induce the residual hearing loss after cochlear implantation. In our previous study, we found that charge-balanced biphasic electrical stimulation inhibited the neurite growth of spiral ganglion neurons (SGNs) and decreased Schwann cell density in vitro. In this study, we want to know whether cochlear implant-based electrical stimulation can induce the change of electrical activity in cultured SGNs. Methods. Spiral ganglion neuron electrical stimulation in vitro model is established using the devices delivering cochlear implant-based electrical stimulation. After 48 h treatment by 50 μA or 100 μA electrical stimulation, the action potential (AP) and voltage depended calcium current (ICa) of SGNs are recorded using whole-cell electrophysiological method. Results. The results show that the ICa of SGNs is decreased significantly in 50 μA and 100 μA electrical stimulation groups. The reversal potential of ICa is nearly +80 mV in control SGN, but the reversal potential decreases to +50 mV in 50 μA and 100 μA electrical stimulation groups. Interestingly, the AP amplitude, the AP latency, and the AP duration of SGNs have no statistically significant differences in all three groups. Conclusion. Our study suggests cochlear implant-based electrical stimulation only significantly inhibit the ICa of cultured SGNs but has no effect on the firing of AP, and the relation of ICa inhibition and SGN damage induced by electrical stimulation and its mechanism needs to be further studied.

show abstract

“…Ca 2+ influx through Ca v 1 channels mediates the prosurvival effects of depolarization in many neurons (Collins et al, 1991) including rat SGNs (Hegarty et al, 1997; Roehm et al, 2008; Shen et al, 2016). To determine if activity-dependent increases in SGN survival differed in WT and C-KO cultures, we used a survival assay modified from previous studies of rat SGNs (Hegarty et al, 1997; Roehm et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

CaBP1 regulates Cav1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons

Yang

Choi

Soh

et al. 2018

Molecular and Cellular Neuroscience

View full text Add to dashboard Cite

Charge-balanced biphasic electrical stimulation inhibits neurite extension of spiral ganglion neurons

Cited by 14 publications

References 28 publications

Electrical stimulation induces synaptic changes in the peripheral auditory system

Electrical stimulation induces synaptic changes in the peripheral auditory system

The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons

CaBP1 regulates Cav1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons

Contact Info

Product

Resources

About