In this letter we show that the Rényi entanglement entropy of a region of large size in a one-dimensional critical model whose ground state breaks conformal invariance (such as in those described by non-unitary conformal field theories), behaves as S n ∼ c eff (n+1) 6n log , where c eff = c − 24∆ > 0 is the effective central charge, c (which may be negative) is the central charge of the conformal field theory and ∆ = 0 is the lowest holomorphic conformal dimension in the theory. We also obtain results for models with boundaries, and with a large but finite correlation length, and we show that if the lowest conformal eigenspace is logarithmic (L 0 = ∆I +N with N nilpotent), then there is an additional term proportional to log(log ). These results generalize the well known expressions for unitary models. We provide a general proof, and report on numerical evidence for a non-unitary spin chain and an analytical computation using the corner transfer matrix method for a non-unitary lattice model. We use a new algebraic technique for studying the branching that arises within the replica approach, and find a new expression for the entanglement entropy in terms of correlation functions of twist fields for non-unitary models.