Ultrasensitive
and accurate detection of biomarkers in serum is
of great importance for disease diagnosis and treatment. So far, the
commonly used single-mode signal suffers from certain instinct drawbacks
that restrict assay performances. Herein, we report the proof-of-concept
fabrication of a split-type photoelectrochemical (PEC) and electrochemical
(EC) dual-modal aptasensor for ultrasensitively tracing tumor necrosis
factor-α, a noteworthy biological biomarker with essential clinical
importance. By smart integrating molybdenum disulfide QDs/zeolitic
imidazolate framework-8@ZnO nanorod arrays with a methylene blue-liposome-mediated
signal amplification strategy, “dual signal-on” detection
is accomplished based on a sandwich reaction of the target with aptamer-anchored
carboxyl magnetic beads and an aptamer-confined MB liposome. Linear
ranges of 5 fg/mL–5 μg/mL (detection limit 1.46 fg/mL)
for PEC and 10 fg/mL–0.5 μg/mL (detection limit 6.14
fg/mL) for EC are obtained, respectively. An independent signal transduction
mechanism supports the accuracy improvement, and a separate biological
process from a translator enables convenient fabrication, short-time
consumption, wider linearity, as well as outstanding reproducibility
and stability in practical application. This work presents a universal
bioassay route with prospects in biomedical and related areas.