Charting the course of renal cryoinjury

Abdulmahdi, Wasan; Zullo, Joseph; Nesi, Lauren; Goligorksy, Michael S.; Ratliff, Brian B.

doi:10.14814/phy2.12357

Cited by 3 publications

(3 citation statements)

References 61 publications

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“…Rodriguez-Porcel et al demonstrated impaired kidney response to vasodilators (acetylcholine and sodium nitroprusside), but they used electron-beam computed tomography, which is difficult to implement routinely. 20 LDF has been extensively used to assess renal microcirculation in mice in ischemiareperfusion, 21,22 preconditioning, 23 renal cryoinjury 24 or other models. 25 LASCA and LDF both use laser speckle patterns, but the analysis techniques differ.…”

Section: Discussionmentioning

confidence: 99%

<p>Assessing Renal Microvascular Reactivity by Laser Speckle-Contrast Imaging in Angiotensin-II-Treated Mice</p>

Mottard

Berkowitz

Santhanam

2020

IJNRD

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Introduction: The kidney is one of the main organs affected by microvascular damage wrought by hypertension. We developed an approach to investigate renal microcirculatory disturbance in live mice by measuring post-occlusive reactive hyperemia (PORH), a reactivity test exploring endothelial and neuro-microvascular functioning. Laser specklecontrast analysis (LASCA) assesses microvascular blood flow; it provides real-time images of spatial and temporal blood flow dynamics. We compared basal blood flow and PORH test between control and angiotensin-II-treated mice (Ang-II) to validate the model. Objective: The study objective was to develop an approach to investigate renal microcirculation, and then to compare microvascular reactivity assessed on LASCA in control versus Ang-II mice. Methods: Thirty 7-week-old wild-type C57BL/6J mice were allocated into two groups. One received angiotensin-II via osmotic minipumps (Ang-II; n=15); the other served as control (n=15). Basal blood flow was measured on LASCA. The PORH test was then performed in the two groups. Results: Control mice had significantly lower basal renal microcirculatory flow, expressed in perfusion units (PU), than Ang-II-treated mice (1448 ± 96 vs 1703 ± 185 PU, respectively; P < 0.05). Peak flow was lower in controls than in Ang-II mice (1617±104 vs.1724 ± 205 PU, respectively; P=0.21). Control mice had significantly higher kidney PORH than Ang-II mice (8±3 vs 1±4%, respectively; P < 0.05). Conclusion: We developed an innovative technique to study renal microcirculation in mice. Ang-II-treated mice showed significantly higher basal blood flow than controls, while PORH was significantly higher in controls than in Ang-II mice.

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Section: Discussionmentioning

confidence: 99%

<p>Assessing Renal Microvascular Reactivity by Laser Speckle-Contrast Imaging in Angiotensin-II-Treated Mice</p>

Mottard

Berkowitz

Santhanam

2020

IJNRD

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“…Certainly, this boasted release of Ccl2 explains for the significant infiltration of macrophages during the early inflammation phase in Ifnar del mice. Under normal conditions, however, this process of activation and recruitment takes three days [ 39 ]. In the current study, we observed that WT mice showed a regular infiltration of regeneration supporting macrophages.…”

Section: Discussionmentioning

confidence: 99%

Loss of Ifnar1 in Pancreatic Acinar Cells Ameliorates the Disease Course of Acute Pancreatitis

et al. 2015

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Type I interferon constitutes an essential component of the combinational therapy against viral disease. Acute pancreatitis is one side effect of type I interferon-based therapy, implying that activation of type I interferon signaling affects the homeostasis and integrity of pancreatic acinar cells. Here, we investigated the role of type I interferon signaling in pancreatic acinar cells using a caerulein-induced murine model of acute pancreatitis. Pancreas-specific ablation of interferon (alpha and beta) receptor 1 (Ifnar1) partially protected animals from caerulein-induced pancreatitis, as demonstrated by reduced tissue damage. Profiling of infiltrating immune cells revealed that this dampened tissue damage response correlated with the number of macrophages in the pancreas. Pharmacologic depletion of macrophages reversed the protective effect of Ifnar1 deficiency. Furthermore, expression of chemokine (C-C motif) ligand 2 (Ccl2), a potent factor for macrophage recruitment, was significantly increased in the Ifnar1-deficient pancreas. Thus, type I interferon signaling in pancreatic acinar cells controls pancreatic homeostasis by affecting the macrophage-mediated inflammatory response in the pancreas.

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“…Mammalian models of acute kidney injury (AKI) have been performed in murine models (Shoskes et al, 1990;Wei and Dong, 2012) and have included surgical approaches to induce ischemia/reperfusion injuries (Duffield et al, 2006) and injection of nephrotoxins such as Cisplatin (Ramesh and Reeves, 2003). In addition, a more recent publication describes a renal cryo-injury model aimed at uncovering endogenous damage and repair processes (Abdulmahdi et al, 2015).…”

Section: The Regenerative Capacity Of the Mammalian Kidneymentioning

confidence: 99%

Kidney regeneration in fish

Bates¹,

Naumann²,

Hoppe³

et al. 2018

Int. J. Dev. Biol.

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Age-related diseases, such as kidney diseases, are becoming more prevalent in aging societies. Currently, patients with reduced kidney function require dialysis or organ transplants. Those who suffer from kidney disease would benefit from regenerative therapies. Thus, one of the ultimate goals of regeneration research is to enhance an individual's capacity of self-repairing damaged tissue; something that fish models can contribute towards. Kidney structures are conserved among vertebrates highlighting the opportunities for fish to act as human disease models. Here, different species can offer respective advantages. An understanding of the different modes of regeneration can help to visualize the differences in mammalian and fish regenerative capacity. The remarkable regenerative capacity of fish is well known, but kidney regeneration is an understudied area. The kinetics of kidney regeneration allows one to investigate early damage responses, as well as the initiation and completion of repair. Age-related reductions in regeneration are an additional societal problem; again an area where fish models can be of help. Age-matched experiments between varied vertebrate species will help us to learn from those that do or do not exhibit age-related phenotypes. The goal of such experiments is not only to outline important age-related factors and pathways, but, in addition, to see if age-related decreases in regenerative capacity can be reduced. Widening our knowledge of this very complex process will help to address many of the unanswered questions in the field.

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Charting the course of renal cryoinjury

Cited by 3 publications

References 61 publications

<p>Assessing Renal Microvascular Reactivity by Laser Speckle-Contrast Imaging in Angiotensin-II-Treated Mice</p>

<p>Assessing Renal Microvascular Reactivity by Laser Speckle-Contrast Imaging in Angiotensin-II-Treated Mice</p>

Loss of Ifnar1 in Pancreatic Acinar Cells Ameliorates the Disease Course of Acute Pancreatitis

Kidney regeneration in fish

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