Chemical analyses on cell envelope polymers of the halophilic, phototrophic Rhodospirillum salexigens

Antibiotics, peptidoglycan synthesis and genomics: the chlamydial anomaly revisited

“…However, if only analytically undetectable amounts of peptidoglycan are present, the polymer cannot have a role in maintaining the structural integrity of EBs or RBs. In the case of the halophilic bacterium Rhodospirillum salexigens, which also has insufficient peptidoglycan to form a structural sacculus, it has been suggested that the polymer occurs in patches around flagellar bases (Evers et al ., 1986), which might imply a role in the stabilization or anchorage of surface appendages to the cell envelope. However, such a role in Chlamydia would appear unlikely in view of the effects of penicillin and cycloserine on cell division.…”

Peptidoglycan-polysaccharide complex in the cell wall of the filamentous prochlorophyte Prochlorothrix hollandica

“…Chemical analyses. Amino acids and amino sugars were determined on an automatic amino acid analyzer (model LC 6001; Biotronik, Munich, Federal Republic of Germany) as described elsewhere (7). Quantitative determination of fatty acids (as methyl ester derivatives) and of neutral sugars and amino sugars (as alditol acetate derivatives) was performed by gas-liquid chromatography as described previously (15).…”

?-ornithine containing peptidoglycan-polysaccharide complex from the cell wall of the gliding bacterium Herpetosiphon aurantiacus

“…Amino acids and amino sugars were determined as described elsewhere [9]. For determination of the amino acid configuration, hydrolysates were esterified with isopropanol/HCl, 1.5 M, and then trifluoroacetylated before separation on a 25 m Chirasil-Val fused-silicacapillary column, coated with XE-60q,-Valine-(S)-I,-phenylethylamide [ 10].…”