Chemical and anticonvulsant screening of crinum jagus

Edema, MO; Okieimen, F. E.

doi:10.4314/njcr.v7i1.35564

Cited by 3 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its local name is "Ogede-Odo" in Yoruba and "Gadali" in Hausa [14]. The plant has previously been reported to have properties such as antibacterial, antifungal [15], anticonvulsant [16], anti-asthmatic [12], and antioxidant activities [8]. It was reported by [17] that methanolic extract of the bulb of Crinum jagus significantly protected mice from death, myconecrosis and haemorrhage induced by the effect of snake venoms.…”

Section: Introductionmentioning

confidence: 99%

Anti-snake Venom Activity of Aqueous and Ethanolic Extracts of Crinum jagus Bulb

Zailani

Magaji

Sarkiyayi

et al. 2018

AJRB

View full text Add to dashboard Cite

Aim: To determine the anti-snake venom effect of crude aqueous and ethanolic extracts and separated fractions of Crinum jagus bulb on rats injected with Echis ocellatus venom. Study Design: Evaluation of LD50 of venom, anti-lethal effects of different extract preparations and their effect on neutralising haemorrhage; the hallmark of Echis ocellatus venom toxicity. Place and Duration of Study: Department of Biochemistry laboratory, Modibbo Adama University of Technology, Yola, Nigeria between March and October 2017. Methodology: venom was collected by the milking method. Fractionation of crude extracts was done by column chromatography separately with benzene/methanol, acetic acid/methanol and ethyl acetate/methanol. Thirty rats were used determine LD50 of venom while twenty-four albino rats were used in the anti-venom study; divided into 6 groups of 4 rats each. Group one served as the normal control, group two served as venom untreated control while groups 3, 4, 5 and 6 were injected with 0.2 mg/mL Echis ocellatus venom and treated with 200 mg/kg body weight crude extracts, fractions I, II and III respectively then observed for 24 hours. Results: LD50 of venom was found to be 5 mg/mL; column chromatography of crude extracts gave three fractions each designated I, II and III respectively. Phytochemical analysis of both crude aqueous and ethanolic extract of Crinum jagus bulb revealed the presence of alkaloids, flavonoids, terpenoids, steroids, tannins and phenolics. Only fraction III of both aqueous and ethanolic extract maintained all phytochemicals from the crude. Both extracts and their fractions were all able to neutralise the venom. For the aqueous extract, the crude extract and fraction III gave the best result while for the ethanolic extract, fractions II and III caused the best neutralisation. Conclusion: Crinum jagus bulb has demonstrated significant anti-venom activity, this can be exploited for the development of new anti-snake venom drugs.

show abstract

Section: Introductionmentioning

confidence: 99%

Anti-snake Venom Activity of Aqueous and Ethanolic Extracts of Crinum jagus Bulb

Zailani

Magaji

Sarkiyayi

et al. 2018

AJRB

View full text Add to dashboard Cite

show abstract

“…Dandy popularly called St. Christopher or Harmattan lily, Frest crinum or Poison bulb is widely used in form of decoction by traditional practitioners in Africa, including Southeastern Nigeria, for treatment of skin wounds and several other ailments [ 26 , 27 , 28 - 32 ] some of which have been scientifically validated [ 33 , 34 , 35 ]. Chemical investigations revealed that it contained high amount of phenolic compounds including crinamine, lycorine, psuedolycorine, 6-hydroxycrinamine, hamayne, tetrahydro-1, 4-oxazine (morpholine), bowdensine, and demethoxy-bowdensine [ 36 , 37 , 38 , 39 ]. It also contained saponins, tannins, calcium oxalate, and calcium tetrata [ 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…Chemical investigations revealed that it contained high amount of phenolic compounds including crinamine, lycorine, psuedolycorine, 6-hydroxycrinamine, hamayne, tetrahydro-1, 4-oxazine (morpholine), bowdensine, and demethoxy-bowdensine [ 36 , 37 , 38 , 39 ]. It also contained saponins, tannins, calcium oxalate, and calcium tetrata [ 37 , 38 ]. Phenolic compounds in plant extracts exhibited enzymatic and non-enzymatic antioxidant effect [ 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antioxidant effect of methanolic Crinum jagus bulb extract in wound healing

Udegbunam¹,

Udegbunam²,

Nnaji³

et al. 2015

J Intercult Ethnopharmacol

View full text Add to dashboard Cite

Aim:The aim of this study was to evaluate the antimicrobial and antioxidant effects of Crinum jagus (J. Thomps.) Dandy methanolic bulb extract in wound healing.Materials and Methods:Phytochemical screening revealed the presence of alkaloids, glycosides, tannins, and saponins in the extract. In vitro antimicrobial activity of the extract was determined by agar well diffusion method. In vivo antimicrobial activity of the extract was determined by microbial assay of excision wound in rats contaminated with Staphylococcus aureus, Bacillus subtilis, Pseudomonas areuginosa, and Candida albicans and treated with 300 mg/kg body weight (bw) of 10 and 5% methanolic C. jagus bulb extract ointment (MCJBEO), respectively. Enzymatic antioxidant effect of the extract was determined in vivo by assaying superoxide dismutase (SOD) and catalase (CAT) activity, and malondialdehyde (MDA) level in excision wound biopsies of rats treated with 10 and 5% MCJBEO, respectively, following standard methods. Non-enzymatic antioxidant effect of the extract was determined in vitro using diphenylpicrylhydrazyl (DPPH) method following standard procedure.Results:The extract exhibited in vitro antimicrobial effect in a concentration-dependent manner with one hundred (100) mg/ml concentration of the extract having the highest inhibitory zone diameter for B. subtilis (25 mm), S. aureus (21 mm), and C. albicans (14 mm) followed by the 50, 25 and 12.5 mg/ml concentrations, respectively. B. subtilis, S. aureus, and C. albicans were not isolated from wounds of animals treated with both extract concentrations 10% and 5% MCJBEO, and reference drug (framycetin sulfate/clotrimazole). Activities of the enzymatic antioxidants SOD and CAT in wound biopsies treated with 10% MCJBEO were significantly (P < 0.05) higher when compared with those treated with 5% MCJBEO. Significantly (P < 0.05) decreased MDA level of wound biopsies from extract-treated rats was observed. The extract exhibited non-enzymatic antioxidant (DPPH) effect in a concentration-dependent manner.Conclusion:This study has shown that an anti-microbial and antioxidant effects could possibly be part of mechanism by which C. jagus bulb extract promote wound healing process.

show abstract

“…jagus bulb showed that it contains alkaloids, polyphenols, and triterpenoids [ 16 ]. Specific compound contained in C. jagus bulb extracts as revealed by chemical investigation included crinamine, lycorine, psuedolycorine, 6-hydroxycrinamine, hamayne, tetrahydro-1, 4-oxazine (morpholine), calcium oxalate, calcium tetrata, bowdensine, and demethoxy-bowdensine [ 16 , 25 , 28 ]. Some of the bioactive compounds contained in C .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of wound healing potential of methanolic Crinum jagus bulb extract

Udegbunam¹,

Kene²,

Anika³

et al. 2015

J Intercult Ethnopharmacol

View full text Add to dashboard Cite

Objective:Crinum jagus (J. Thomps.) Dandy commonly called Harmattan or St. Christopher’s lily belonging to the family Liliaceae is widely used traditionally in Southeastern Nigeria for treatment of skin sores. This study investigated the wound healing potentials of methanolic C. jagus bulb extract (MCJBE) using incision, excision, and dead space wound healing models.Materials and Methods:Phytochemical screening showed the presence of alkaloids, glycosides, tannins, saponins in the extract, but absence of flavonoids. In the incision and dead space wound models, rats were dosed orally with 300 mg/kg body weight (bw) of 10 and 5% of MCJBE solution, respectively, while in the excision wound model, rats were treated topically with 10 and 5% MCJBE ointments (MCJBEO), respectively.Result:The 10% MCJBE gave significantly (P < 0.05) highest percentage rate of wound contraction, shortest re-epithelialization and complete healing time when compared with 5% MCJBE and reference drug, framycetin sulfate. The extract of C. jagus showed significant (P < 0.05) concentration-dependent wound healing activity in incision, dead space and excision wound models. No contaminating microbial organism was isolated from wound sites of the rats dosed and treated with MCJBE throughout the study period. At day 7, post infliction of excision wound, histomorphological, and histochemical studies revealed more fibroblasts and Type 1 collagen deposits in wound site sections of rats treated with both 10 and 5% MCJBEO while those of the control showed more inflammatory cells and fewer Type 1 collagen deposits. At day 14 post infliction of excision wound, more epithelial regeneration with overlying keratin were seen in the histological sections of wounds of rats treated with both 10 and 5% MCJBEO, while histochemical study showed more Type 1 collagen deposits in wound site sections of rats in 10% MCJBEO treated group.Conclusion:This study established that methanolic C. jagus bulb extract potentiates wound healing. The study thus validated the folkloric use of C. jagus bulb in the management of skin sores and boils.

show abstract

Chemical and anticonvulsant screening of crinum jagus

Cited by 3 publications

References 2 publications

Anti-snake Venom Activity of Aqueous and Ethanolic Extracts of Crinum jagus Bulb

Anti-snake Venom Activity of Aqueous and Ethanolic Extracts of Crinum jagus Bulb

Antimicrobial and antioxidant effect of methanolic Crinum jagus bulb extract in wound healing

Evaluation of wound healing potential of methanolic Crinum jagus bulb extract

Contact Info

Product

Resources

About