Chemical and biological characterization of four new linear cationic α-helical peptides from the venoms of two solitary eumenine wasps

Rangel, Marisa; Cabrera, Marcia Perez dos Santos; Kazuma, Kohei; Ando, Kenji; Wang, Xiaoyu; Kato, Manabu; Nihei, Ken Ichi; Hirata, Izaura Yoshico; Cross, Tyra J.; Garcia, Angélica Nunes; Faquim-Mauro, Eliana L.; Franzolin, Márcia Regina; Fuchino, Hiroyuki; Mori‐Yasumoto, Kanami; Sekita, Setsuko; Kadowaki, Makoto; Satake, Motoyoshi; Konno, Katsuhiro

doi:10.1016/j.toxicon.2011.04.014

Cited by 40 publications

(58 citation statements)

References 42 publications

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“…In the presence of anionic asolectin vesicles, the spectrum shows some deviation of the helical features, which could indicate the contribution of aggregated peptides. Nevertheless, the spectra do not indicate a preferential interaction with anionic environments as already found with other antimicrobial peptides [34,35,36]. …”

Section: Resultssupporting

confidence: 50%

“…The giant unilamellar vesicles were prepared from asolectin (Sigma-Aldrich Co., USA) and formed by electroswelling, using the device Vesicle Prep Pro (Nanion Technologies GmbH, Germany) following the protocol described by Rangel et al, 2011 [34]. Lipid bilayers were formed from GUVs after added to the borosilicate glass chips NPC-1 aperture.…”

Section: Methodsmentioning

confidence: 99%

“…Lipid bilayers were formed from GUVs after added to the borosilicate glass chips NPC-1 aperture. Electrical measurements were performed with the automated Patch-Clamp device Port-a-Patch® (Nanion Technologies GmbH, Germany) as previously described [34]. …”

Section: Methodsmentioning

confidence: 99%

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Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

et al. 2017

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The rapid spread of multi-drug resistant pathogens represents a serious threat to public health, considering factors such as high mortality rates, treatment restrictions and high prevalence of multi-drug resistant bacteria in the hospital environment. Antimicrobial peptides (AMPs) may exhibit powerful antimicrobial activity against different and diverse microorganisms, also presenting the advantage of absence or low toxicity towards animal cells. In this study, the evaluation of the antimicrobial activity against multi-drug resistant bacteria of a recently described AMP from wasp, Polydim-I, was performed. Polydim-I presented activity against standard strains (non-carriers of multi-resistant genes) that are susceptible to commercial antimicrobials, and also against multi-drug resistant strains at concentrations bellow 1μg/ml (0.41 μM). This is a rather low concentration among those reported for AMPs. At this concentration we found out that Polydim-I inhibits almost 100% of the tested pathogens growth, while with the ATCC strains the minimum inhibitory concentration (MIC100) is 400 times higher. Also, in relation to in vitro activity of conventional drugs against multi-drug resistant bacteria strains, Polydim-I is almost 10 times more efficient and with broader spectrum. Cationic AMPs are known as multi-target compounds and specially for targeting the phospholipid matrix of bacterial membranes. Exploring the interactions of Polydim-I with lipid bilayers, we have confirmed that this interaction is involved in the mechanism of action. Circular dichroism experiments showed that Polydim-I undergoes a conformational transition from random coil to a mostly helical conformation in the presence of membrane mimetic environments. Zeta potential measurements confirmed the binding and partial charge neutralization of anionic asolectin vesicles, and also suggested a possible aggregation of peptide molecules. FTIR experiments confirmed that some peptide aggregation occurs, which is minimized in the presence of strongly anionic micelles of sodium dodecyl sulfate. Also, Polydim-I induced channel-like structures formation to asolectin lipid bilayers, as demonstrated in the electrophysiology experiments. We suggest that cationic Polydim-I targets the membrane lipids due to electrostatic attraction, partially accumulates, neutralizing the opposite charges and induces pore formation. Similar mechanism of action has already been suggested for other peptides from wasp venoms, especially mastoparans.

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Section: Resultssupporting

confidence: 50%

Section: Methodsmentioning

confidence: 99%

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Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

et al. 2017

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“…The balance between antimicrobial and hemolytic effects has been discussed in numerous works [116,[140][141][142][143][144][145][146][147][148][149][150][151]. The hemolytic effect is especially pronounced for Ltc 2a (Table 4).…”

Section: Hemolysismentioning

confidence: 99%

Latarcins: versatile spider venom peptides

Dubovskii

Vassilevski

Kozlov

et al. 2015

Cell. Mol. Life Sci.

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Arthropod venoms feature the presence of cytolytic peptides believed to act synergetically with neurotoxins to paralyze prey or deter aggressors. Many of them are linear, i.e., lack disulfide bonds. When isolated from the venom, or obtained by other means, these peptides exhibit common properties. They are cationic; being mostly disordered in aqueous solution, assume amphiphilic α-helical structure in contact with lipid membranes; and exhibit general cytotoxicity, including antifungal, antimicrobial, hemolytic, and anticancer activities. To suit the pharmacological needs, the activity spectrum of these peptides should be modified by rational engineering. As an example, we provide a detailed review on latarcins (Ltc), linear cytolytic peptides from Lachesana tarabaevi spider venom. Diverse experimental and computational techniques were used to investigate the spatial structure of Ltc in membrane-mimicking environments and their effects on model lipid bilayers. The antibacterial activity of Ltc was studied against a panel of Gram-negative and Gram-positive bacteria. In addition, the action of Ltc on erythrocytes and cancer cells was investigated in detail with confocal laser scanning microscopy. In the present review, we give a critical account of the progress in the research of Ltc. We explore the relationship between Ltc structure and their biological activity and derive molecular characteristics, which can be used for optimization of other linear peptides. Current applications of Ltc and prospective use of similar membrane-active peptides are outlined.

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“…The release of the granular b-hexosaminidase enzyme was measured based on previous work (Rangel et al, 2011) in the supernatants obtained from RBL-2H3 rat mast cell lineage. For this, 2 Â 10 5 cells were diluted in Tyrode's buffer, distributed (160 mL/well) in round-bottom plates (Costar) and maintained for 2 h at 37 C in a humidified 5% CO 2 incubator.…”

Section: In Vitro Mast Cell Degranulation Induced By Pmvmentioning

confidence: 99%

Mast cells and histamine play an important role in edema and leukocyte recruitment induced by Potamotrygon motoro stingray venom in mice

Kimura

Prezotto-Neto

Távora

et al. 2015

Toxicon

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Chemical and biological characterization of four new linear cationic α-helical peptides from the venoms of two solitary eumenine wasps

Cited by 40 publications

References 42 publications

Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

Latarcins: versatile spider venom peptides

Mast cells and histamine play an important role in edema and leukocyte recruitment induced by Potamotrygon motoro stingray venom in mice

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