Chemical and biological functionalization of titanium for dental implants

Schliephake, Henning; Scharnweber, Dieter

doi:10.1039/b715355b

Cited by 122 publications

(92 citation statements)

References 145 publications

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“…1 To this end, surface modifications in general and immobilization of biomolecules in particular are extensively investigated strategies to enhance these biological responses. 2 Generally, techniques used for the immobilization of biomolecules at material surfaces can be categorized into either covalent or noncovalent strategies. 3 Noncovalent immobilization typically involves simple dip-coating treatments that make noncovalent immobilization strategies relatively fast and cheap.…”

mentioning

confidence: 99%

1-Step Versus 2-Step Immobilization of Alkaline Phosphatase and Bone Morphogenetic Protein-2 onto Implant Surfaces Using Polydopamine

Nijhuis

Beucken²,

Boerman³

et al. 2013

Tissue Engineering Part C: Methods

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Immobilization of biomolecules onto implant surfaces is highly relevant in many areas of biomaterial research. Recently, a 2-step immobilization procedure was developed for the facile conjugation of biomolecules onto various surfaces using self-polymerization of dopamine into polydopamine. In the current study, a 1-step polydopamine-based approach was applied for alkaline phosphatase (ALP) and bone morphogenetic protein-2 (BMP-2) immobilization, and compared to the conventional 2-step polydopamine-based immobilization and plain adsorption. To this end, ALP and BMP-2 were immobilized onto titanium and polytetrafluoroethylene (PTFE) substrates. The absolute quantity and biological activity of immobilized ALP were assessed quantitatively to compare the three types of immobilization. Plain adsorption of both ALP and BMP-2 was inferior to both polydopamine-based immobilization approaches. ALP was successfully immobilized onto titanium and PTFE surfaces via the 1-step approach, and the immobilized ALP retained its enzymatic activity. Using the 1-step approach, the amount of immobilized ALP was increased twofold to threefold compared to the conventional 2-step immobilization process. In contrast, more BMP-2 was immobilized using the conventional 2-step immobilization approach. Retention of ALP and BMP-2 was measured over a period of 4 weeks and was found to be similar for the 1-step and 2-step methods and far superior to the retention of adsorbed biomolecules due to the formation of covalent linkages between catechol moieties and immobilized proteins. The biological behavior of ALP and BMP-2 coatings immobilized using polydopamine (1- and 2-step) as well as adsorption was assessed by culturing rat bone marrow cells, which revealed that the cell responses to the various experimental groups were not statistically different. In conclusion, the 1-step polydopamine-based immobilization method was shown to be more efficient for immobilization of ALP, whereas the conventional 2-step method was shown to be more efficient for attachment of BMP-2 onto implant surfaces.

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mentioning

confidence: 99%

1-Step Versus 2-Step Immobilization of Alkaline Phosphatase and Bone Morphogenetic Protein-2 onto Implant Surfaces Using Polydopamine

Nijhuis

Beucken²,

Boerman³

et al. 2013

Tissue Engineering Part C: Methods

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show abstract

“…Для решения проблемы улучшения взаимодействия имплантата с костью на сегодня предложены многочисленные модификации, в частности изменение морфологии и состава поверхности материала для увеличения адгезии остеогенных клеток и их пролиферации [6]. Для этого используют покрытие имплантата гидроксиапатитом, формирование нано-и микроструктурованой поверхности [5,11].…”

Section: Introductionunclassified

Microhardness of Bone Tissue After Different Alloy Implantation

2015

European Journal of Medicine. Series B

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Integral indicator of bone quality is microhardness, which depends both on the condition of the inorganic component and the quality of the extracellular organic matrix. There are a lot of researches indicated microhardness changes around the metal implants, but it is not clear how it change distant from injured place.The aim of our research was to determine the microhardness of the femoral bone at different terms after the implantation of metal alloys with different composition.65 rabbits were random in 5 groups -intact, control (bone defect) and 3 experimental. We used TiVT6 alloy with high elasticity module and β-(Ti-Zr) alloy with low Young module for the experimental groups. All alloys were implanted in distal epiphysis of femur.Bone microhardness was detected in periimplanted zone as well as in middle of diaphysis and proximal epiphysis.Injury of bone leads to a decrease in the hardness in the zone of the defec as well as in distal zones, followed by recovery up to 6 month. Use the classic high-modulus alloy TiVT6 leads to a significant decrease in the microhardness of all sections of bone in the early and late postoperative period. Implantation of low modulus alloy β (Ti-Zr) leads to a reduction of hardness just after 1 and 3 months after the injury, and the use of hydroxyapatite coating significantly reduces the loss of bone quality at all times after implantation.

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“…Organic surface modifications that are currently investigated involve immobilization of among others structural proteins, signaling molecules, enzymes or peptides onto biomaterial surfaces to target cell response at the tissue-implant interface [74,75].…”

Section: Trends In Materials For Organic Coatings On Bone Implantsmentioning

confidence: 99%

Surface Engineering for Bone Implants: A Trend from Passive to Active Surfaces

et al. 2012

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Abstract:The mechanical and biological properties of bone implants need to be optimal to form a quick and firm connection with the surrounding environment in load bearing applications. Bone is a connective tissue composed of an organic collagenous matrix, a fine dispersion of reinforcing inorganic (calcium phosphate) nanocrystals, and bone-forming and -degrading cells. These different components have a synergistic and hierarchical structure that renders bone tissue properties unique in terms of hardness, flexibility and regenerative capacity. Metallic and polymeric materials offer mechanical strength and/or resilience that are required to simulate bone tissue in load-bearing applications in terms of maximum load, bending and fatigue strength. Nevertheless, the interaction between devices and the surrounding tissue at the implant interface is essential for success or failure of implants. In that respect, coatings can be applied to facilitate the process of bone healing and obtain a continuous transition from living tissue to the synthetic implant. Compounds that are inspired by inorganic (e.g., hydroxyapatite crystals) or organic (e.g., collagen, extracellular matrix components, enzymes) components of bone tissue, are the most obvious candidates for application as implant coating to improve the performance of bone implants. This review provides an overview of recent trends and strategies in surface engineering that are currently investigated to improve the biological performance of bone implants in terms of functionality and biological efficacy. OPEN ACCESSCoatings 2012, 2 96

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Chemical and biological functionalization of titanium for dental implants

Cited by 122 publications

References 145 publications

1-Step Versus 2-Step Immobilization of Alkaline Phosphatase and Bone Morphogenetic Protein-2 onto Implant Surfaces Using Polydopamine

1-Step Versus 2-Step Immobilization of Alkaline Phosphatase and Bone Morphogenetic Protein-2 onto Implant Surfaces Using Polydopamine

Microhardness of Bone Tissue After Different Alloy Implantation

Surface Engineering for Bone Implants: A Trend from Passive to Active Surfaces

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