Chemical and Genetic Differentiation of <i>Ligularia pleurocaulis</i> in Northwestern Yunnan and Southwestern Sichuan Provinces of China

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The intraspecific diversity in L. tsangchanensis collected in the Chinese Provinces Yunnan and southwestern Sichuan was studied by chemical and genetic approaches. The samples collected in Yunnan were found to contain cacalol (1) as the sole major component, while samples from Sichuan contained 7alpha- and 7beta-eremophila-9,11-dien-8-one (5 and 6) as well as the 3alpha-angeloyloxy derivative 7 as major components. In addition, the sequences of the internal transcribed spacers (ITSs) of the ribosomal RNA gene indicated that the Yunnan and the Sichuan samples constitute separate clades. These results demonstrate that L. tsangchanensis in Yunnan and Sichuan are distinct.

supporting

confidence: 90%

“…Hand.-Mazz. of northwestern Yunnan is distinct, both chemically and genetically, from the same species found in southwestern Sichuan [3]; 2) the species L. virgaurea (Maxim.) Mattf.…”

mentioning

confidence: 96%

Chemical and Genetic Differentiation of Ligularia tsangchanensis in Yunnan and Sichuan Provinces of China

Torihata

Hanai

Gong

et al. 2007

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“…The San-shion sample collected and analyzed some 30 years ago, as mentioned above, contained guaiane derivatives and furanoligularinone, the latter of which is positive to Ehrlichs test [6] [11]. However, neither guaiane nor any Ehrlich-positive compounds were found in the samples of the present study.…”

Section: Chemical and Genetic Diversity Of Ligularia Latihastata And mentioning

confidence: 38%

Chemical and Genetic Diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China

Kuroda

Kiuchi

Torihata

et al. 2007

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The chemical constituents of the root extracts and the nucleotide sequences of the atpB-rbcL intergenic region of Ligularia latihastata and L. villosa, collected in northwestern Yunnan Province, were studied. In the twelve collected samples of L. latihastata, two major benzofurans, 5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran (1) and euparin (2) were detected as major components. The minor compound (2R*,3S*)-5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl (2Z)-2-[(acetoxy)methyl]but-2-enoate (4) was found to be susceptible to artifact formation upon extraction with EtOH. The intra-specific diversity in chemical composition of the samples was small, but the diversity in the atpB-rbcL sequence was fairly large. Compounds 1 and 2 were also found in the three collected samples of L. villosa, indicating that the two species are chemically close to each other, in agreement with morphological taxonomy.

“…In one type (A in Table 1), guanine (G), cytosine (C), and adenine (A) were at the 245th, the 301st, and the 469th positions, respectively, and the number of thymines (T) in a stretch around the 390th position was 9. This type was the same as in L. cymbulifera [2], L. tongolensis [2], L. pleurocaulis [3], and L. virgaurea var. virgaurea [4].…”

mentioning

confidence: 63%

High Diversity of Ligularia dictyoneura in Chemical Composition and DNA Sequence

Nagano

Iwazaki

Matsushima

et al. 2007

Self Cite

The chemical composition of root extracts of the title species collected at 20 different places in the Hengduan Mountains of China was examined. From these samples, a total of 17 eremophilane derivatives were isolated, three of which were new franoeremophilane derivatives: 3beta-acetoxy-6beta-(angeloyloxy)furanoeremophilan-10beta-ol, 1alpha-acetoxyfuranoeremophilan-15,6alpha-olide, and 6beta-[2-(hydroxymethyl)prop-2-enoyloxy]furanoeremophil-1(10)-ene. Based on the chemical composition, the samples could be classified into as many as seven types: one type containing non-furanoeremophilane derivatives and the other six containing furanoeremophilane derivatives with different oxidation levels. Results of DNA sequencing of the atpB-rbcL region and the internal transcribed spacers of the ribosomal RNA gene also indicated a high diversity in the species.