Chemical and genetic probes for analysis of protein palmitoylation

Li, Lun; Dong, Linjie; Xia, Lisha; Li, Tingting; Zhong, Hongying

doi:10.1016/j.jchromb.2010.11.018

Cited by 9 publications

(5 citation statements)

References 113 publications

(132 reference statements)

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“…Palmitoylated oncogenic NRAS is a proposed target for developing therapies against NRAS-associated malignancies like acute myeloid leukemia (AML) as well as other types of NRAS-amplified leukemias [ 96 ]. With such a contradictory role for the palmitoylation, researchers have developed novel probes that can be utilized for the fluorescence microscopy and mass spectrometry analysis of protein palmitoylation [ 97 ].…”

Section: Palmitoylationmentioning

confidence: 99%

Small Changes Huge Impact: The Role of Protein Posttranslational Modifications in Cellular Homeostasis and Disease

Karve

Cheema

2011

Journal of Amino Acids

309

234

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Posttranslational modifications (PTMs) modulate protein function in most eukaryotes and have a ubiquitous role in diverse range of cellular functions. Identification, characterization, and mapping of these modifications to specific amino acid residues on proteins are critical towards understanding their functional significance in a biological context. The interpretation of proteome data obtained from the high-throughput methods cannot be deciphered unambiguously without a priori knowledge of protein modifications. An in-depth understanding of protein PTMs is important not only for gaining a perception of a wide array of cellular functions but also towards developing drug therapies for many life-threatening diseases like cancer and neurodegenerative disorders. Many of the protein modifications like ubiquitination play a decisive role in various drug response(s) and eventually in disease prognosis. Thus, many commonly observed PTMs are routinely tracked as disease markers while many others are used as molecular targets for developing target-specific therapies. In this paper, we summarize some of the major, well-studied protein alterations and highlight their importance in various chronic diseases and normal development. In addition, other promising minor modifications such as SUMOylation, observed to impact cellular dynamics as well as disease pathology, are mentioned briefly.

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Section: Palmitoylationmentioning

confidence: 99%

Small Changes Huge Impact: The Role of Protein Posttranslational Modifications in Cellular Homeostasis and Disease

Karve

Cheema

2011

Journal of Amino Acids

309

234

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“…Although radioactive labeling is effective for quantitation, the procedure is tedious, potentially toxic, and time-consuming. 7 Moreover, it is incapable of specifying the palmitoylation sites, unless mutations are made 8 or antibodies that target regions of interest are used in conjunction with enzymatic digestion. 9 A mass spectrometry (MS) compatible method for palmitoylation analysis has recently been developed, which utilizes the acylbiotinyl exchange (ABE) chemistry to irreversibly replace the thioester-linked palmitoyl modifications with stable biotin tags.…”

mentioning

confidence: 99%

“…In early days, during decades of research, radioactive labeling was used for detection of palmitoylation. Although radioactive labeling is effective for quantitation, the procedure is tedious, potentially toxic, and time-consuming . Moreover, it is incapable of specifying the palmitoylation sites, unless mutations are made or antibodies that target regions of interest are used in conjunction with enzymatic digestion .…”

mentioning

confidence: 99%

Direct Detection of S-Palmitoylation by Mass Spectrometry

Leymarie

Haeussler

et al. 2013

Anal. Chem.

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Direct detection and quantification of protein/peptide palmitoylation by mass spectrometry (MS) is a challenging task because of the tendency of palmitoyl loss during sample preparation and tandem MS analysis. In addition, the large difference in hydrophobicity between the palmitoyl peptides and their unmodified counterparts could prevent their simultaneous analysis in a single liquid chromatography-MS experiment. Here, the stability of palmitoylation in several model palmitoyl peptides under different incubation and fragmentation conditions was investigated. It was found that the usual trypsin digestion protocol using dithiothreitol as the reducing agent in ammonium bicarbonate buffer could result in significant palmitoyl losses. Instead, it is recommended that sample preparation be performed in neutral Tris buffer with tris(2-carboxyethyl)phosphine as the reducing agent, conditions under which palmitoylation was largely preserved. For tandem MS analysis, collision-induced dissociation often led to facile palmitoyl loss, and electron capture dissociation frequently produced secondary side-chain losses remote from the backbone cleavage site, thus discouraging their use for accurate palmitoylation site determination. In contrast, the palmitoyl group was mostly preserved during electron transfer dissociation, which produced extensive inter-residue cleavage coverage, making it the ideal fragmentation method for palmitoyl peptide analysis. Finally, derivatization of the unmodified peptides with a perfluoroalkyl tag, N-[(3-perfluorooctyl)propyl] iodoacetamide, significantly increased their hydrophobicity, allowing them to be simultaneously analyzed with palmitoyl peptides for relative quantification of palmitoylation.

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“…This bacterium enhances offspring production, especially females, by modulating reproductive development through cytoplasmic incompatibility (J. Li et al, 2023b). This trait benefits the bacterium as it is transmitted vertically (Bi & Wang, 2020).…”

Section: Resultsmentioning

confidence: 99%

Whitefly distribution and interaction with endosymbionts in the state of Paraná

Tomaz,

Cobianchi,

Lima

et al. 2023

Sem. Ci. Agr.

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Whiteflies comprise a complex of cryptic species that are genetically distinct but morphologically indistinguishable. Among them, Bemisia tabaci and Trialeurodes vaporariorum (Tvap) are the most prominent species causing agricultural damage. In Brazil, three B. tabaci species have been identified: New World (NW), Middle East-Asia Minor (MEAM1), and Mediterranean (MED). Whiteflies establish symbiotic associations with endosymbiotic bacteria, conferring adaptive advantages to the insects. The objective of this study was to identify whitefly species in municipalities of the state of Paraná and investigate their interactions with endosymbiotic bacteria. Insects were collected in 14 municipalities across different edaphoclimatic regions of Paraná, either in agricultural crops or on weeds. Molecular analyses, based on previously published PCR-based methods, were used to identify the insects and their respective endosymbionts. The results revealed that B. tabaci MEAM1, MED, NW, and Tvap accounted for 39.8%, 27.0%, 6.3%, and 23.7% of the collected specimens, respectively. These insects exhibited interactions with endosymbiont genera such as Portiera, Arsenophonus, Rickettsia, Hamiltonella, and Wolbachia, either in isolation or through co-infection. In some instances, interactions with up to five endosymbionts were observed in specific locations. The occurrence of Tvap in open fields and the prevalence of MED, particularly in the southeast of the state, where temperatures are milder, along with their interactions with endosymbionts providing adaptive advantages to the insects, raise significant phytosanitary concerns for the agricultural security of Paraná.

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Chemical and genetic probes for analysis of protein palmitoylation

Cited by 9 publications

References 113 publications

Small Changes Huge Impact: The Role of Protein Posttranslational Modifications in Cellular Homeostasis and Disease

Small Changes Huge Impact: The Role of Protein Posttranslational Modifications in Cellular Homeostasis and Disease

Direct Detection of S-Palmitoylation by Mass Spectrometry

Whitefly distribution and interaction with endosymbionts in the state of Paraná

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