Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films

Gnapareddy, Bramaramba; Dugasani, Sreekantha Reddy; Ha, Taewoo; Paulson, Bjorn; Hwang, Taehyun; Kim, Taesung; Kim, Jae-Hoon; Oh, Kyunghwan; Park, Sungha

doi:10.1038/srep12722

Cited by 54 publications

(37 citation statements)

References 28 publications

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“…5D) as a function of PEG concentration, the intensities of the peaks increased up to a certain concentration and then began to decrease with further increase in PEG concentration along with that of DOX. In this context, we can conclude that the presence of PEG in the DOX-PEG 500 -epoxy solutions manifests a quenching effect on DOX, depending on PEG concentration, which indicate on the one hand that DOX was bounded to PEG molecule 43 and on the other hand, being also suitable to involve hydrogen bonds with PEG moieties 44 . The emission intensities of DOX-PEG 500 -epoxy, AuPEG 2000 -DOX and iAuPEG 2000 -DOX solutions at higher DOX concentration (around 0.2 mg/mL) are in the same range (Fig.…”

Section: Resultsmentioning

confidence: 80%

Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells

Lupușoru

Pricop

Uritu

et al. 2020

Sci Rep

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The paper aims to investigate the cytotoxic effect on tumor cells of irradiated AuNPs in green light and subsequently functionalized with HS-PEG-NH 2. The toxicity level of gold conjugates after their functionalization with DOX and TAT peptide was also evaluated. The AuNPs were prepared using the modified Turkevich method and exposed to visible light at a wavelength of 520 nm prior their PEGylation. The optical properties were analyzed by UV-vis spectroscopy, the surface modification was investigated using FTIR and XPS spectroscopies and their sizes and morphologies were evaluated by TEM and DLS techniques. DOX and TAT peptide were linked to the surface of PEGylated AuNPs by reacting their amino groups with glycidyloxypropyl of PEGylated DOX or TAT conjugates under mild conditions at room temperature and in the presence of ethanol as catalyst. The conjugates containing DOX or DOX and TAT have been characterized by fluorescence and FTIR techniques. The changes of electrochemical features were observed using cyclic voltammetry, suggesting a better stability of irradiated nanoparticles. By mass spectrometry it was confirmed that the compounds of interest were obtained. The cell viability test showed that irradiated and non-irradiated nanoparticles coated with PEG are not toxic in normal cells. Tumor cell viability analysis showed that the PEGylated nanoparticles modified with DOX and TAT peptide were more effective than pristine DOX, indicating cytotoxicity up to 10% higher than non-irradiated ones. The presence of gold nanomaterials (AuNPs) in biomedicine and particularly in antitumor therapy still remains a topic of wide debate, as evidenced by the tremendous amount of scientific works on this issue in recent years 1-3. An impressive number of research studies have straightened their efforts toward the use of AuNPs in enhancing the efficiency of cancer treatment, due to their ease production and chemical functionalization of their surface 4,5. Gold nanoparticles are feasible to be developed as versatile nontoxic carriers for drug release as long as they are able to be conjugated with different molecules, including chemotherapeutics, antibodies, peptides, ligands, and other structures which are likely to promote a great capacity to penetrate the tumor site, resulting in a predominant accumulation of bioactive agent in the tumor region 6,7. On the other hand, the passive anticancer effect based on the accumulation strategy of AuNPs at the tumor site is limited by the inherent heterogeneities of tumor vasculature 8. It was shown that nanoparticle concentration in the target tissue is influenced by renal clearance rate, and also by activation of immune system mechanisms such as opsonization or nonspecific particle phagocytosis, fulfilled by the reticuloendothelial system (RES).

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Section: Resultsmentioning

confidence: 80%

Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells

Lupușoru

Pricop

Uritu

et al. 2020

Sci Rep

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“…Since the denaturation process breaks the hydrogen bonds between nucleobases, we focused on how one of the nucleobases, cytosine, would change in its characteristic vibrational peaks for various NaOH concentrations. It is well known that in pristine DNA the cytosine inplane vibration has an absorption peak at ∼1489 cm −1 [31,32], and for the cytosine-guanine bond vibration band it is at ∼1419 cm −1 [31]. It is observed that these two peaks showed significant spectral shifts as the NaOH concentration increased or, equivalently, the relative denaturation increased in the solution precursors.…”

Section: B Ftir Measurement To Confirm Denaturation In Thin Solid Filmmentioning

confidence: 97%

“…Figure 4(a) shows the FTIR absorption spectra of the freestanding solid DNA films with various NaOH concentrations in the solution precursors. The FTIR absorption spectra were divided into three spectral regions: 3600 − 3000 cm −1 for OH stretching, 1800 − 1300 cm −1 for nucleobase vibrations, and 1250 − 700 cm −1 for the sugar and phosphate backbone groups [31]. Since the denaturation process breaks the hydrogen bonds between nucleobases, we focused on how one of the nucleobases, cytosine, would change in its characteristic vibrational peaks for various NaOH concentrations.…”

Section: B Ftir Measurement To Confirm Denaturation In Thin Solid Filmmentioning

confidence: 99%

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Jeong¹,

Paulson²,

Hong³

et al. 2018

Photon. Res.

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The denaturation of double-stranded deoxyribonucleic acid (ds-DNA) has been well known to break nucleobase bonds, resulting in single-stranded deoxyribonucleic acid (ss-DNA) in solutions, which can recombine to form ds-DNA in a reversible manner. We developed an efficient process to irreversibly maintain various DNA denaturation levels in thin solid films in order to investigate the impacts of the denaturation on the optical properties of DNA films. By adding NaOH in an aqueous solution of salmon testis DNA, we flexibly controlled the level of denaturation in the solution, which was then spin-coated on Si and silica substrates to irreversibly bind ss-DNAs in a thin solid film. The denaturation of DNA in thin solid films was experimentally confirmed by ultravioletvisible and Fourier transform infrared spectroscopic investigations, whose level could be controlled by the NaOH content in the aqueous solution precursor. By this irreversible denaturation process, we developed a new method to flexibly vary the refractive index of DNA thin solid films in a wide range of Δn > 0.02 in the visible to nearinfrared range. Thermo-optic coefficients dn/dT of the films were also experimentally measured in the temperature range from 40°C to 90°C to confirm the significant impacts of denaturation. Detailed thin film processes and optical characterizations are discussed.

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“…The signicant Raman spectra of DNA nucleotides are categorized into two regions: 400-750 cm À1 for nucleobases and 750-1200 cm À1 for sugar phosphate backbone groups. 21,22 Due to a certain limitation when taking Raman spectra through the synthetic DX tiles-exceeding the Raman signal of the 1Â TAE/ Mg 2+ buffer (40 mM Tris base, 20 mM acetic acid, 1 mM EDTA (pH 8.0), and 12.5 mM magnesium acetate) rather than the signal of the DNA molecules-we alternatively obtained them from the natural DNA duplexes extracted from salmon, which are also valid because the frequency range compared with the simulation is higher than 300 cm À1 . In that relatively high frequency range, the DNA molecule generally shows the local vibrational characteristics of backbones and specic bases.…”

Section: Resultsmentioning

confidence: 99%

Vibrational characteristics of DNA nanostructures obtained through a mass-weighted chemical elastic network model

Son

Lee

et al. 2017

RSC Adv.

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Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films

Cited by 54 publications

References 28 publications

Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells

Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Vibrational characteristics of DNA nanostructures obtained through a mass-weighted chemical elastic network model

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