Chemical Aspects of Light.

Bowen, E. J.; Lind, S. C.

doi:10.1021/j150450a012

Cited by 55 publications

(23 citation statements)

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“…An alternative interpretation could be founded on the assumption, that the binding of the dye to the cell alters the structural symmetry of the dye molecule and produces changes in the orbital pathways of the oscillating electron. This view seems to be supported by the evidence when the absorption spectra of the symmetrical crystal violet and unsymmetrical malachite green are compared (10).…”

Section: Absorption Of Methylene Blue By Bacteria and The Rate Of Phomentioning

confidence: 77%

Studies on the Mechanism of the Photosensitized Inactivation of E. Coli and Reactivation Phenomenon

Heinmets¹,

Vinegar²,

Taylor³

1952

Journal of General Physiology

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Photosensitized inactivation of microorganisms has been the subject of numerous studies, and various hypothetical reaction mechanisms have been proposed for the interpretation of the phenomenon. In spite of such extensive experimentation and theorizing, there still exists uncertainty concerning the inactivation processes. No attempt will be made to review the subject since it has been analyzed and treated by Blum (1) and Arnow (2). It is evident that photosensitized inactivation is dependent on a variety of experimental factors. However, the literature reveals that the influence of such factors has been determined in many diverse and isolated experiments which makes the over-all evaluation of the data difficult, It was thought that, by studying a single system under various experimental conditions, a more satisfactory interpretation could be found. A methylene blue and E. coli system was selected, since the methylene blue oxidation-reduction cycle represents a reversible process, and the associated structural changes in the molecule are known. Experimental Metkods and Results Experimental Material and Irradiation Procedures.--E. coli B/r were grown inliquid synthetic M-9 medium (3) with aeration. The culture was centrifuged and resuspended in the desired solvent. Mter irradiation, assays were made by diluting the suspension in saline and plating on nutrient agar containing 4 × 10 -6 gin. per ml. of gentian violet.G. E. H-4 and DH-1 mercury lamps were used as irradiation sources. The test tubes containing the samples were placed in a water bath 5 cm. from the source. During the irradiation oxygen bubbled through the solution, providing a constant oxygen tension and continuous agitation of the suspension. Occasionally, different procedures were used. These will be described in other sections of the paper.The methylene blue used was u.s.1,, grade commercial product which contained not less than 98.5 per cent methylene blue chloride.

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Section: Absorption Of Methylene Blue By Bacteria and The Rate Of Phomentioning

confidence: 77%

Studies on the Mechanism of the Photosensitized Inactivation of E. Coli and Reactivation Phenomenon

Heinmets¹,

Vinegar²,

Taylor³

1952

Journal of General Physiology

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“…These processes are investigated in "photochemical research,, 255,256,381,485,492,687,761,762,1077,1295,1298,1335,1445,1623,1814,1928,2014,2316,2317,2396,2399).…”

Section: Interaction Of Low Energy Photons With Mattermentioning

confidence: 99%

ESR Spectroscopy in Polymer Research

Rånby¹,

Rabek²

1977

Polymers Properties and Applications

295

118

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“…This lamp was used only when extensive irradiation was needed that would have required a prohibitively long exposure to the low pressure lamp. Intensities or radiation were measured actinoinetrically by the method based on the use of uranyl oxalate as described by Bowen (1946). With the high pressure lamp, whose radiation is polychromatic, the intensity was computed as if all the radiation was of 254 mp.…”

Section: Materials a N D Methodsmentioning

confidence: 99%

Effects of Ultraviolet Radiation on Antigenicity of Horse Serum Albumin: Formation of New Determinants

Kleczkowski¹,

Gold²

1962

Photochem & Photobiology

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Summary Structural alterations in horse serum albumin caused by ultraviolet radiation resulted in disappearance of the original determinants and in formation of new determinants. There was, however, enough similarity between the original and the new determinants for antibodies produced by the new determinants to show a weak affinity to the non‐irradiated albumin. Only weak antisera were produced by injecting rabbits with extensively irradiated albumin.

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Chemical Aspects of Light.

Cited by 55 publications

References 0 publications

Studies on the Mechanism of the Photosensitized Inactivation of E. Coli and Reactivation Phenomenon

Studies on the Mechanism of the Photosensitized Inactivation of E. Coli and Reactivation Phenomenon

ESR Spectroscopy in Polymer Research

Effects of Ultraviolet Radiation on Antigenicity of Horse Serum Albumin: Formation of New Determinants

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