Chemical Assay of Iron in Ovarian Cysts: A New Diagnostic Method to Evaluate Endometriotic Cysts

Iizuka, Mari; Igarashi, Masao; Abe, Yumiko; Ibuki, Yoshito; Koyasu, Yasuki; Ikuma, K

doi:10.1159/000009999

Cited by 30 publications

(16 citation statements)

References 8 publications

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“…al. [40] have reported that endometriotic cyst iron content ranges from 100–1.7 mM. Patients with endometriosis who develop endometriomas may have heavy or prolonged bleeding into these cysts and peritoneal implants thus exposing more heme proteins to HOCl generated locally by MPO leading to the generation of free iron mediated by HOCl-hemeprotein heme destruction [8, 31, 33, 41].…”

Section: Discussionmentioning

confidence: 99%

Impact of hydrogen peroxide-driven Fenton reaction on mouse oocyte quality

Shaeib¹,

Banerjee²,

Maitra³

et al. 2013

Free Radical Biology and Medicine

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Here we show that hydroxyl radical (•OH) generated through the Fenton reaction alters metaphase-II mouse oocyte microtubules (MT) and chromosomal alignment (CH). Metaphase–II mouse oocytes, obtained commercially, were grouped as follows: control, hydrogen peroxide (H2O2), Fe (II), and combined (Fe (II) + H2O2) treatments. After 7–10 minutes of incubation, at 37 °C, MT and CH were evaluated on fixed and stained oocytes and scored by two blinded observers. Pearson Chi-square test, Fisher's Exact test were used to compare outcomes between controls and treated groups, and also amongst each group. Our results showed that poor scores for MT and CH increased significantly in oocytes treated with combination of H2O2 and Fe(II) (p <0.001); oocytes treated with H2O2 alone or Fe(II) alone showed no or little changes compared to control. Comparison of oocyte groups that received increasing concentrations of H2O2 and fixed amount of Fe (II) showed that 70 – 80 % demonstrated poor scores both in MT and CH when pretreated with 5 μM H2O2, and increased up to 90–100% when treated with 10–20 μM of H2O2. Hydroxyl radical generated by H2O2 driven Fenton reaction deteriorates the metaphase-II mouse oocyte spindle and CH alignment, which is thought to be a potential cause of poor oocyte quality. Thus, free iron and/or ROS scavengers could attenuate the •OH-mediated spindle and chromosomal damage, thereby serving as a possible approach for further examination as a therapeutic option in inflammatory states.

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Section: Discussionmentioning

confidence: 99%

Impact of hydrogen peroxide-driven Fenton reaction on mouse oocyte quality

Shaeib¹,

Banerjee²,

Maitra³

et al. 2013

Free Radical Biology and Medicine

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“…Other tumor markers, metabolites, or hormones such as CEA, Ca 125, Ca 19.9, alphafetoprotein, progesterone, or iron can also be assayed, 18,19 but they lack sensitivity and specificity. In our series, 81.2% of follicular cysts had an E2 content Ͼ4,000 pg/ml (Fig.…”

Section: Discussionmentioning

confidence: 99%

Value of ultrasound-guided fine-needle aspiration in the management of ovarian and paraovarian cysts

Allias¹,

Chanoz²,

Blache³

et al. 2000

Diagn. Cytopathol.

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This study was designed to assess if cytology was accurate for an appropriate diagnosis of ovarian and paraovarian cysts, and if the ultrasound‐cytology‐estradiol (UCE) triad was sufficient to discriminate functional vs. nonfunctional cysts, the latter requiring surgical resection. One hundred twenty‐two ultrasound‐diagnosed adnexal cysts were punctured and surgically removed, and then subjected to cytologic and histologic examinations; 90 of these fluids were assayed for estradiol. Histologically, 30 cysts were functional and 92 were nonfunctional. A correct discrimination between functional and nonfunctional origin was obtained in 54.9% of cases with cytology, in 94.4% with estradiol assay, in 50.8% with ultrasonography, and in 97.8% with these three examinations combined (UCE triad). Among the 34 patients with no criteria of neoplastic origin (age >40, ultrasonographic findings), the UCE triad diagnosed six functional cysts. Therefore, 17.6% (6/34) of these young women could have avoided unnecessary surgery. Diagn. Cytopathol. 2000;22:70–80. © 2000 Wiley‐Liss, Inc.

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“…Endometriotic cysts contain a huge amount of free iron. The concentration exceeds 100 nmol/L, which is up to 10 000‐fold higher than the concentration normally present in serum (0.013–0.027 mmol/L) . Accordingly, massive iron staining has also been observed in the stroma of the cyst, mostly located in macrophages .…”

Section: Introductionmentioning

confidence: 93%