Chemical binding of horseradish peroxidase enzyme with poly beta‐cyclodextrin and its application as molecularly imprinted polymer for the monitoring of H₂O₂ in human plasma samples

Abstract

In this study, a selective and sensitive molecular imprinting-based electrochemical sensors, for horseradish peroxidase (HRP) entrapment was fabricated using electro polymerization of ß-Cyclodextrin (ß-CD) on the surface of glassy carbon electrode. Poly beta-cyclodextrin P(ß-CD) provide efficient surface area for self-immobilization of HRP as well as improve imprinting efficiency. The proposed imprinted biosensor successfully utilized for detection of HRP with excellent analytical results which linear range is 0.1 mg/mL to 10 ng/mL with LOD of 2.23 ng/mL. Furthermore, electrocatalytical activity of the prepared biosensor toward the reduction of hydrogen peroxide was investigated in the ranges of 1 to 15 μM with a detection limit of 0.4 μM by using chronoamperometry technique. The developed biosensor was used for the detection of hydrogen peroxide in unprocessed human plasma sample.

Solid Phase-Based Microextraction Techniques in Therapeutic Drug Monitoring

“…Another possible application of the MIP extraction procedure is the work of Sardaremelli et al [97] for monitoring hydrogen peroxide in plasma samples, fully validated and applied to unprocessed biological samples. Abdollahiyan et al [98] have used MIPS for the identification of carcinoembryonic antigens in plasma samples, concluding that the developed extraction technique appears to be suitable for point-of-care applications in biomedical and clinical analysis.…”

Green Aspects in Molecularly Imprinted Polymers by Biomass Waste Utilization

“…Recently, some works combining the advantages of metal nanomaterials with the selectivity of MIPs were published [146][147][148][149][150]. [136] with permission from Wiley.…”

“…A recent work describes a molecularly imprinted polymer sensor for horseradish peroxidase (HRP) prepared through an electropolymerization of ß-CDs on the surface of a glassy carbon electrode [ 136 ]. In this study, Poly beta-cyclodextrins P(ß-CDs) were used as supporting material and the reaction was carried out combining electropolymerization of ß-CDs and the molecular imprinted technology.…”

“… MIP/P(β-CD)/GCE preparation and detection of HRP. Reproduced from the work in [ 136 ] with permission from Wiley. …”

Chemical binding of molecular‐imprinted polymer to biotinilated antibody: Utilization of molecular imprinting polymer as intelligent synthetic biomaterials toward recognition of carcinoma embryonic antigen in human plasma sample

“… 10‐12 . In comparison with the other diagnostic techniques, MIPs, which comprise three main unique characters of application universality, recognition specificity, and structure predictability, have found broad interest and have become useful in several areas, such as separation and purification, 13 bio/chemosensing, 14 drug delivery, 15 artificial antibodies, 16 and catalysis, 17 respect to their straightforward preparation, high physical stability, affordability, and remarkable robustness 18 . Bulk polymerization is a conventional method for molecular imprinting.…”